HPLC wavelength switching method for simultaneously determining gallic acid content and ziyuglycoside I content in sanguisorba officinalis l or sanguisorba officinalis l preparation

A technology of gallic acid and burnet saponin, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not being able to fully control burnet white tablets, and achieve the effect of quality control guarantee and comprehensive active ingredient content

Active Publication Date: 2017-08-18
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE +1
View PDF6 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tan Peng et al. [14] used HPLC-ELSD method to separately determine the content of Burnet saponin I in Diyu Shengbai Tablets. The single determination of Sanyu saponin I in this document cannot fully control the content of Diyu Shengbai Tablets.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HPLC wavelength switching method for simultaneously determining gallic acid content and ziyuglycoside I content in sanguisorba officinalis l or sanguisorba officinalis l preparation
  • HPLC wavelength switching method for simultaneously determining gallic acid content and ziyuglycoside I content in sanguisorba officinalis l or sanguisorba officinalis l preparation
  • HPLC wavelength switching method for simultaneously determining gallic acid content and ziyuglycoside I content in sanguisorba officinalis l or sanguisorba officinalis l preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1 HPLC wavelength switching method and methodology verification of the present invention

[0061] 1 Instruments, reagents and medicines

[0062] SSI series 1500 high performance liquid chromatograph (USA SSI company), ultraviolet detector, CSChrom Plus chromatographic workstation; chromatographic column Inertsil ODS-3 (250mm×4.6mm, 5μm, GL Sciences lnc.); KQ-600DE type numerical control ultrasonic cleaning Device (40KHz, 600W); 1 / 100,000 electronic balance (Ohaus, Switzerland DV-215-CD); Youpu UPT series ultrapure water device (Chengdu Youpu Electronic Products Co., Ltd.), adjustable pipette ( American Thermo, Finnpipette series, 20-200 μL, 100-1000 μL).

[0063] Burnet (see Table 1 for the batch number, purchased in Qingshui County, Tianshui City, Gansu Province); Burnet Burnet (see Table 2 for the batch number, provided by Chengdu Di’ao Pharmaceutical Group Co., Ltd.); gallic acid reference substance (lot number: M-017 -150129, purchased from Chengdu Ruif...

Embodiment 2

[0099] The screening of embodiment 2 chromatographic conditions of the present invention

[0100] 1. Selection of target compound wavelength

[0101] Precisely draw the mixed reference solution and inject it into the high performance liquid chromatograph, and use the secondary array tube detector to scan the full wavelength within 200-400nm. I has an absorption maximum at 210nm (see image 3 , Figure 4 ), while gallic acid has maximum absorption near 210nm and 280nm (see figure 2 , Figure 4), but it is not conducive to the separation of impurity peaks and the calculation of content at 210nm. Therefore, this study adopts the switching wavelength method to detect and analyze the test samples, firstly select 280nm to avoid impurity peaks, and then switch to 210nm to detect Burnet elm saponins. For the 3D spectrum of Burnet reference substance, see Figure 5 .

[0102] 2. Selection of mobile phase ratio

[0103] The selected mobile phase system is acetonitrile-0.05% pho...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
separationaaaaaaaaaa
Login to view more

Abstract

The invention discloses an HPLC wavelength switching method for simultaneously determining the gallic acid content and the ziyuglycoside I content in sanguisorba officinalis l or a sanguisorba officinalis l preparation. The HPLC wavelength switching method comprises: (1) establishing a standard curve of gallic acid and ziyuglycoside I: a, preparating a reference substance solution, and b, determining the reference substance solution; and (2) determining the gallic acid content and the ziyuglycoside I content in a sample to be detected: c, preparing a sample solution, and d, determining the sample solution. According to the present invention, the HPLC wavelength switching method for simultaneously determining the gallic acid content and the ziyuglycoside I content in the sanguisorba officinalis l or the sanguisorba officinalis l preparation is successfully established, the gallic acid content and the ziyuglycoside I content can be simply, rapidly, accurately and simultaneously determined, the active component content in the sanguisorba officinalis l or the sanguisorba officinalis l preparation can be completely monitored, the intrinsic quality of the drug can be accurately reflected, and the effective assurance is provided for the quality control of the sanguisorba officinalis l or the sanguisorba officinalis l preparation.

Description

technical field [0001] The invention relates to the detection of medicines, in particular to an HPLC wavelength switching method for simultaneously measuring the contents of gallic acid and burnet saponin I in burnet or burnet preparations. Background technique [0002] Burnet in Burnet and Burnet preparations is the dry root of Sanguisorba officinalis L. or Sanguisorba officinalis L.Var.longifolia (Bert.) Yüet Li, a plant in the Rosaceae family. Efficacy of sores [1]. Burnet mainly contains polyphenols, saponins and flavonoids, and the polyphenols mainly include gallic acid, (+)-catechin, ellagic acid, and gallocatechin [2-4]. Burnet has the effect of increasing the number of white blood cells. It is often used as an adjuvant drug during chemotherapy to treat leukopenia caused by chemotherapy [5-7], and it can also be used to treat leukopenia caused by psychotropic drugs [8], interference The leukopenia caused by hepatitis B can be treated with antibiotics[9,10]. [0003...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 邓赟胡佳李莎潘文慧宋川霞郭大乐
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap