HPLC method for simultaneously determining gallic acid content and (+)-catechin content in radix sanguisorbae preparation

A technology of gallic acid and catechin, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problem that there is no report on the content determination method of gallic acid and (+)-catechin in the Burnet preparations, and there is no Burnet liter content. Reports on the content determination method of gallic acid and (+)-catechin in white tablets, etc., to achieve the effect of moderate requirements, low cost of purchase and use, and excellent linear relationship

Inactive Publication Date: 2016-07-06
CHENGDU DIAO PHARMA GROUP
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  • Abstract
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Problems solved by technology

[0006] However, there is no report on the determination method of gallic acid and (+)-catechin content in Burnet eucalyptus preparations, let alone the determination method of gallic acid and (+)-catechin content in Diyu Shengbai Tablets to report

Method used

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  • HPLC method for simultaneously determining gallic acid content and (+)-catechin content in radix sanguisorbae preparation
  • HPLC method for simultaneously determining gallic acid content and (+)-catechin content in radix sanguisorbae preparation
  • HPLC method for simultaneously determining gallic acid content and (+)-catechin content in radix sanguisorbae preparation

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Embodiment 1

[0039] Embodiment 1 HPLC method of the present invention and methodology verification

[0040] 1. Simultaneous determination of gallic acid and (+)-catechin in Diyu Shengbai tablets

[0041] 1 Instruments, reagents and medicines

[0042] SSIseries1500 high performance liquid chromatograph (USA SSI company), ultraviolet detector, CSChromPlus chromatographic workstation; chromatographic column InertsilODS-3 (250mm×4.6mm, 5μm, GLScienceslnc.); KQ-600DE type numerical control ultrasonic cleaner (40KHz, 600W) ; 1 / 100,000 electronic balance (Switzerland Ohaus DV-215-CD); Youpu UPT series ultra-pure water device (Chengdu Youpu Electronic Products Co., Ltd.).

[0043] Diyu Shengbai Tablets (batch numbers 141215, 141111, 140811, 141208, provided by Chengdu Di'ao Pharmaceutical Group Co., Ltd.); gallic acid reference substance (batch number: 11083-201204, purchased from China National Institute of Pharmaceutical and Biological Products), (+) - Catechin reference substance (batch numbe...

Embodiment 2

[0079] The screening of embodiment 2 chromatographic conditions of the present invention

[0080] (1) Selection of wavelength

[0081] DAD full-wavelength scanning, the DAD spectrum of gallic acid is as follows Figure 5 As shown, the DAD spectrum of (+)-catechin is shown in Figure 6 shown.

[0082] The results show that (+)-catechin has a strong absorption band in addition to the terminal absorption, and the corresponding lambda value is 280nm. Detection wavelengths for both components.

[0083] (2) Screening of gradient elution program, column temperature, flow rate and chromatographic column

[0084] The inventors investigated the gradient elution procedure, column temperature, flow rate and chromatographic column.

[0085] During the investigation, the main factors were first investigated, namely the gradient elution program. When investigating the gradient elution program, general parameters were adopted for the column temperature, flow rate, and chromatographic col...

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Abstract

The invention discloses a HPLC method for simultaneously determining the gallic acid content and the (+)-catechin content in a radix sanguisorbae preparation.The gallic acid content and the (+)-catechin content can be determined simply, conveniently, accurately and simultaneously, wherein the detection limit of gallic acid reaches 0.62 ng, the average recovery rate of the gallic acid reaches 98.99%, the detection limit of (+)-catechin reaches 12.20 ng, and the average recovery rate of the (+)-catechin reaches 101.09%.Therefore, the method has the advantages that the active ingredient content in the radix sanguisorbae preparation can be more comprehensively monitored, the inherent quality of medicine is more truly reflected, and an effective guarantee is supplied to quality control over the radix sanguisorbae preparation.

Description

technical field [0001] The invention relates to the detection of medicines, in particular to an HPLC method for simultaneously determining the contents of gallic acid and (+)-catechin in burnet preparations. Background technique [0002] Burnet (Latin scientific name: Sanguisorba officinalis L.) is a perennial herbaceous plant of the genus Burnet in the family Rosaceae. It has a spindle-shaped stout heel, short-stalked leaflets, purple-red petals, and the fruit is contained in the calyx tube. The aliases are "Huangzhuxiang", "Yuzha", "Yuqi" or "Sour Zhe". Distributed in the northern temperate zone of Asia, widely distributed in Europe and China, it grows in areas with an altitude of 30 meters to 3000 meters, often grows in shrubs, hillside grasslands, grasslands, meadows and sparse forests, and has been artificially introduced and cultivated. [0003] Burnet elm is also a Chinese herbal medicine, cold in nature, bitter and sour in taste, non-toxic; it returns to the liver, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 邓赟胡佳王泽宇广兵康敏李鸿翔
Owner CHENGDU DIAO PHARMA GROUP
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