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Method for determining vitamin b12 uptake

A vitamin and B12 technology, used in biological testing, pharmaceutical formulations, biological material analysis, etc.

Inactive Publication Date: 2017-08-18
SOC DES PROD NESTLE SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These measurements are often used in combination because none of the methods alone has sufficient sensitivity and specificity

Method used

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  • Method for determining vitamin b12 uptake
  • Method for determining vitamin b12 uptake
  • Method for determining vitamin b12 uptake

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0202] Example 1 - Increase in Serum Amnion Levels Correlates with Age

[0203] Using the Somalogic technique to identify age-related changes in rat serum proteins, the inventors found that the serum levels of rat amniotic proteins increased significantly with age ( figure 1 ).

[0204] The level of cobalamin in rat serum was measured using a competitive binding immunoluminescence enzyme assay, and the results showed that the level of total vitamin B12 decreased with increasing age ( figure 2 ). This is consistent with increasing levels of amnion-free assayed in serum. Because the hepatic pool of vitamin B12 is known to supply vitamin B12 to the rest of the body for several weeks when vitamin B12 intake or absorption is low, at 18 months of age, before the reduction in circulating vitamin B12 levels that occurs at 20 months of age The fact that amnion levels increased suggests that changes in amnion can lead to age-related vitamin B12 insufficiency.

Embodiment 2

[0205] Example 2 - Changes in amnion-free levels are associated with decreased muscle mass

[0206] It was further observed that amnion-free and vitamin B12 changes in rat serum occurred above 18 months of age, corresponding to the onset of muscle mass decline observed in hindlimb muscles of aged rats. For the gastrocnemius, image 3 is illustrated in . Preliminary in vitro experiments demonstrated that the tissue VitB12 receptor CD320 is expressed on differentiated muscle cells but not in their proliferating progenitor cells ( Figure 4 ), suggesting that vitamin B12 can enter skeletal muscle and affect muscle physiology.

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Abstract

The present invention relates to a method for determining a level of intestinal uptake of vitamin B12 in a subject comprising: - determining a level of the cubam complex or a component thereof in an isolated sample from the subject; and - comparing the level of the cubam complex or a component thereof to reference levels; wherein the level of the cubam complex or a component thereof compared to the reference levels is indicative of the level intestinal uptake of vitamin B12.

Description

technical field [0001] The present invention relates to the field of undernutrition. In particular, the present invention relates to methods for determining intestinal absorption of vitamin B12 in a subject. Background technique [0002] Vitamin B12 or cobalamin is a cobalt-containing water-soluble vitamin that cannot be synthesized by the human body, so it must be ingested from food or synthesized by intestinal flora (Hughes et al.; Ann Clin Biochem, 2013.50(Pt 4) : p.315-29 (Hughes et al., Annals of Clinical Biochemistry, 2013, Vol. 50, Part 4, pp. 315-329)). The vitamin B12 pool in the human body consists of several forms: cyanocobalamin, which is inactive and requires conversion for activity; and methylcobalamin and adenosylcobalamin, which are metabolically active derivatives of vitamin B12. [0003] After detachment from the food matrix in the stomach, vitamin B12 binds to intrinsic factor (IF) in the small intestine where it passes through a specific heterodimer cal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/714G01N33/68G01N33/82
CPCA61K31/714G01N33/6893G01N33/82G01N2800/06A61P1/04A61P3/02A61P3/10A61P7/06A61P9/00A61P19/10A61P25/28A61P43/00A23V2002/00A61K9/0053A61K35/744A61K35/745A61K35/747A61K36/064A61K2035/11A23L33/15A23L33/30
Inventor J·费热A·帕内雷克
Owner SOC DES PROD NESTLE SA