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Preparation method of hemostatic material based on fibrin gel

A technology of fibrin gel and hemostatic materials, which is applied in the field of preparation of hemostatic materials, can solve the problems of inability to achieve rapid hemostasis, side effects, complex formulas, etc., and achieve rich cell growth information, promoted cell formation, and no immune response Effect

Inactive Publication Date: 2017-08-22
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These hemostatic materials cannot achieve rapid hemostasis in people with low platelets and procoagulant factors
There are also some products whose hemostasis principle is to simulate the blood coagulation reaction of the human body to stop bleeding, but these products have many raw materials, complex formulas, and the introduction of high molecular polymers, which may cause immune rejection by the human body and have side effects

Method used

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  • Preparation method of hemostatic material based on fibrin gel
  • Preparation method of hemostatic material based on fibrin gel
  • Preparation method of hemostatic material based on fibrin gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of a hemostatic material based on fibrin gel, the steps are as follows:

[0026] A dissolves bovine fibrinogen

[0027] Dissolve bovine fibrinogen with 0.9% NaCl solution at a temperature of 37°C to obtain a bovine fibrinogen solution with a concentration of 5 mg / mL;

[0028] B lysing human thrombin

[0029] At 37°C, with 330mol / L CaCl 2 The solution dissolves human thrombin to obtain a concentration of 3*10 4 Unit / L of human thrombin solution;

[0030] Synthesis of C gel

[0031] At a temperature of 37°C, mix the fibrinogen solution in step A and the thrombin solution in step B at a volume ratio of 1:1 to obtain a mixture, then apply the mixture dropwise on gauze, and then place it on a shaker at 37°C Reaction 4h, that is.

Embodiment 2

[0033] A preparation method of a hemostatic material based on fibrin gel, the steps are as follows:

[0034] A dissolves bovine fibrinogen

[0035] Dissolve bovine fibrinogen with 0.9% NaCl solution at a temperature of 30°C to obtain a bovine fibrinogen solution with a concentration of 10 mg / mL;

[0036] B lysing human thrombin

[0037] At 30°C, with 440mol / L of CaCl 2 The solution dissolves human thrombin to obtain a concentration of 5*10 4 Unit / L of human thrombin solution;

[0038] Synthesis of C gel

[0039] At a temperature of 30°C, mix the fibrinogen solution in step A and the thrombin solution in step B at a volume ratio of 1:3 to obtain a mixture, then apply the mixture dropwise on gauze, and then place it on a shaker at 30°C Reaction 8h, that is.

Embodiment 3

[0041] A preparation method of a hemostatic material based on fibrin gel, the steps are as follows:

[0042] A dissolves bovine fibrinogen

[0043] Dissolve bovine fibrinogen with 0.9% NaCl solution at a temperature of 50°C to obtain a bovine fibrinogen solution with a concentration of 30 mg / mL;

[0044] B lysing human thrombin

[0045] At a temperature of 50°C, with 660mol / L of CaCl 2 The solution dissolves human thrombin to obtain a concentration of 7*10 4 Unit / L of human thrombin solution;

[0046] Synthesis of C gel

[0047] At a temperature of 50°C, mix the fibrinogen solution in step A and the thrombin solution in step B according to a volume ratio of 1:5 to obtain a mixture, then apply the mixture dropwise on gauze, and then place it on a shaker at 50°C Reaction 12h, that is.

[0048] Figure 1a It is the 45min platelet adhesion fluorescence picture of the blank slide. Figure 1b , Figure 1c , Figure 1d 45min platelet adhesion fluorescence images of the hemos...

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Abstract

The invention discloses a preparation method of a hemostatic material based on fibrin gel. The preparation method comprises the following steps: A. dissolution of bovine fibrinogen: at the temperature of 30-50 DEG C, bovine fibrinogen is dissolved by the use of a 0.9% NaCl solution so as to obtain a bovine fibrinogen solution with the concentration of 5-30 mg / Ml; B. dissolution of human thrombin: at the temperature of 30-50 DEG C, human thrombin is dissolved by the use of 330-660 mol / L of a CaCl2 solution so as to obtain a human thrombin solution with the concentration of 3-7 * 104 unit / L; and C. synthesis of gel: at the temperature of 30-50 DEG C, the fibrinogen solution obtained in the Step A and the thrombin solution obtained in the Step B are mixed according to the volume ratio of 1:1-5 so as to obtain mixed liquor; and the mixed liquor is dropped on gauze and then reacts on a shaker of 30-50 DEG C for 4-12 h. The hemostatic material prepared by the method has a good hemostatic effect, has good biocompatibility, can promote formation of granulation tissues and epithelial tissues and speed up healing and repairing of skin tissues, is beneficial to observation of wounds and healing situation, and is convenient for diagnosis and treatment of wounds.

Description

technical field [0001] The invention relates to a preparation method of a hemostatic material based on fibrin gel. Background technique [0002] There are many kinds of hemostatic materials currently available, and the hemostatic principle of most products is to use compression to stop bleeding by relying on platelets and coagulation-promoting factors in the own blood. These hemostatic materials cannot achieve rapid hemostasis in people with low platelets and procoagulant factors. There are also some products whose hemostasis principle is to simulate the blood coagulation reaction of the human body to stop bleeding, but these products have many raw materials, complex formulas, and the introduction of high molecular polymers, which may cause immune rejection by the human body and have side effects. Contents of the invention [0003] The purpose of this invention is to provide a kind of preparation method of hemostatic material based on fibrin gel, the hemostatic effect of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/106A61L24/0015A61L24/0031A61L24/0042A61L24/108A61L2300/254A61L2300/412A61L2300/418A61L2300/604A61L2400/04
Inventor 陈俊英高霞丽谭建英黄思悦魏来黄楠
Owner SOUTHWEST JIAOTONG UNIV
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