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Method for simultaneously measuring contents of arctiin, glycyrrhizic acid and pulegone

A technology of pulegone and arctiin, applied in the field of pulegone content, simultaneous determination of arctiin and glycyrrhizin in traditional Chinese medicine extracts and their preparations, can solve the problems of no simultaneous determination

Inactive Publication Date: 2017-08-22
SHENZHEN NEPTUNUS PHARMA RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In "Chinese Pharmacopoeia" (2015 edition), there are methods for determining the content of arctiin in arctium, glycyrrhizic acid in licorice, and pulegone in Nepeta (ear), but there is no simultaneous determination of arctiin, glycyrrhizic acid, and pedicel. The method for the content of menthone, in order to better develop and utilize the classic ancient prescription "Duwei San", the present invention has carried out in-depth research on its quality control, and established a method for simultaneously determining the content of arctiin, glycyrrhizic acid and pulegone

Method used

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  • Method for simultaneously measuring contents of arctiin, glycyrrhizic acid and pulegone
  • Method for simultaneously measuring contents of arctiin, glycyrrhizic acid and pulegone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Determination of Arctiin, Glycyrrhizic Acid, and Pulegone in Xiaoer Yanning Oral Liquid (prepared by Shenzhen Neptune Pharmaceutical Technology Research Institute Co., Ltd., batch number: 20160401)

[0028] 1) Chromatographic conditions and system adaptability test:

[0029] Octadecylsilane-bonded silica gel was used as filler (250×4.6mm, 5μm); the mobile phase was methanol-acetonitrile-phosphoric acid aqueous solution as the mobile phase, and the gradient elution was shown in the table below.

[0030]

[0031] The detection wavelengths are: 280nm (arctiin), 237nm (glycyrrhizin), 252nm (pulegone), and the number of theoretical plates is not less than 3000 based on the peak of arctiin.

[0032] 2) Preparation of the test solution: Accurately measure 3ml of Xiaoeryanning Oral Liquid, put it in a 25ml volumetric flask, add 70% aqueous methanol to the mark, shake well, filter, and get final product.

[0033] 3) Preparation of reference substance solution: take...

Embodiment 2

[0035] Example 2 Methodology verification for the content determination of arctiin, glycyrrhizic acid, and pulegone in Xiaoeryanning Oral Liquid (prepared by Shenzhen Neptune Pharmaceutical Technology Research Institute Co., Ltd., batch number: 20160401)

[0036] 1) Specificity Under the optimal chromatographic conditions, the chromatographic peaks of arctiin, glycyrrhizic acid, and pulegone in Xiaoeryanning Oral Liquid were well separated without interference, indicating that the method has good specificity. See figure 1 .

[0037] 2) Linearity Accurately prepare different concentrations of arctiin, ammonium glycyrrhizinate, and pulegone reference substance solutions. Accurately draw 20 μL of the above-mentioned reference substance solution, measure according to the chromatographic conditions determined above, take the injection volume (X) of each reference substance as the abscissa, and the peak area integral value (Y) as the ordinate, draw a standard curve, and obtain 3 T...

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Abstract

The invention discloses a method for simultaneously measuring contents of arctiin, glycyrrhizic acid and pulegone in traditional Chinese medicine. The method comprises the steps that firstly, arctigenin, glycyrrhizinate and pulegone standard substances and a sample containing fructus arctii, licorice root and schizonepeta spikes (or schizonepeta) are taken respectively to be dissolved in a methanol aqueous solution, and a standard solution and a sample solution are prepared respectively; secondly, the standard solution and the sample solution which are identical in volume are taken to be injected into an efficient liquid chromatograph, then the peak area of the standard solution and the test solution is measured, and the contents of arctiin, glycyrrhizic acid and pulegone in the sample are obtained. The quick and accurate method is provided for measuring the contents of arctiin, glycyrrhizic acid and pulegone in traditional Chinese medicine containing fructus arctii, licorice root and schizonepeta spikes (or schizonepeta) and in extracts or preparations of the traditional Chinese medicine.

Description

technical field [0001] The invention relates to a method for simultaneously measuring the contents of arctiin, glycyrrhizic acid and pulegone in Chinese medicine extracts and preparations thereof, and belongs to the technical field of Chinese medicines. Background technique [0002] Pharyngitis belongs to the category of "throat numbness" in traditional Chinese medicine. Modern medicine thinks that pharyngitis is the acute inflammation of the pharyngeal mucosa caused by pathogenic infections such as hemolytic streptococcus, staphylococcus, pneumococcus. Traditional Chinese medicine believes that it is mostly caused by heat and evil poison that invades the throat, internally injures the lungs, and persists, resulting in sore throat; The main clinical manifestations are pharyngeal redness, pain, dry itching, discomfort and foreign body sensation, often accompanied by fever. The disease is acute inflammation of the pharynx with the main clinical manifestations of sore throat,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/042
Inventor 谭道鹏王晶江东龙卢禁曾伟珍
Owner SHENZHEN NEPTUNUS PHARMA RES INST CO LTD
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