Preparation method of measles virus lysate as coating antigen of ELISA kit

Abstract

The invention discloses a preparation method of a measles virus lysate used as an enzyme-linked reagent kit for coating antigens. The attenuated measles virus strain is used to cultivate and harvest the measles virus liquid, and the concentrated measles virus liquid is separated by ultrafiltration or other methods, and added to the measles virus lysate. The virus antigen protection solution is adjusted to a certain concentration of the measles virus stock solution and stored for later use. After lysis, the concentration is adjusted with the sample diluent to obtain the measles virus lysate, which is directly used as the coating antigen. After adjusting the pH value (pH value) of the coating and fixative solution and ionic strength, select the appropriate buffer pair, improve the sensitivity and specificity of detection, and meet the needs of clinical detection.

Description

technical field [0001] The invention relates to the field of clinical experiments, in particular to a method for preparing a measles virus lysate used as an enzyme-linked kit for coating antigens. Background technique [0002] Morbillivirus belongs to Family Paramyxoviridae (Family Paramyxoviridae) and Morbillivirus (Genus Morbillivirus). It is a negative-strand RNA virus. It is different from other paramucosal viruses in that it has no special neuraminidase and is spherical in shape with a diameter of only 100-250nm. , Helical symmetry, enveloped, containing hemagglutinin on the envelope. The total length of the genome is about 16kb. The genome has six genes, N, P, M, F, H, and L, which encode six structural and functional proteins: nucleoprotein NP, phosphorylated protein P, membrane protein M, fusion protein F (fusion protein, F), hemagglutinin protein H (haemagglutinin protein, H), RNA-dependent RNA polymerase (Large polymerase, L). Their components are all glycoprotein...

AI Technical Summary

Problems solved by technology

The cost of using recombinant antigens to replace natural whole virus antigens is higher, and the structural and functional protein fragments of recombinant measles virus expressed by genetic engineering techniques often have incomplete epitope components

In addition, the recombinant antigen obtained by prokaryotic expression, separation and purification of E. coli contains other miscellaneous proteins of E. coli. However, in people who have been infected by E. coli, the anti-E. coli antibodies in the serum can react with these miscellaneous proteins and cause false positives. These miscellaneous proteins are likely to affect the detection results