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Pulsatilla saponin b 4 Application in the preparation of medicines for treating pain

A technology of Pulsatilla saponin and medicine, which is applied in the field of pharmaceutical use, and can solve the problems that the pain effect of Pulsatilla saponin B4 has not been reported in literature, etc.

Active Publication Date: 2020-07-14
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pulsatilla saponin B has also been reported in recent years 4 Anti-liver cancer research, but for Pulsatilla saponin B 4 The pain effect has not been reported in the literature

Method used

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  • Pulsatilla saponin b <sub>4</sub> Application in the preparation of medicines for treating pain
  • Pulsatilla saponin b <sub>4</sub> Application in the preparation of medicines for treating pain
  • Pulsatilla saponin b <sub>4</sub> Application in the preparation of medicines for treating pain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 50 ICR mice of 18-22 g, certificate number: SCYK ​​(苏) 2013-0003, were randomly divided into 4 groups, namely the acetic acid writhing model group, B 4 Low-dose group (12.5mg / kg), middle-dose group (25mg / kg) and high-dose group (50mg / kg), positive control group.

[0051] The dosages of the administration groups were 12.5 mg / kg, 25 mg / kg, and 50 mg / kg, respectively. The positive control drug was aspirin. The mice were adapted to the laboratory environment 1 day in advance, and the mice in the treatment group were injected intraperitoneally with Pulsatilla saponin B 1 hour in advance 4 , the model group was given the same amount of normal saline, and 0.6%-1% acetic acid solution was injected intraperitoneally, and the number of writhing times of each mouse within 20 minutes was observed and recorded. Writhing in mice is manifested by extended hindlimbs, concave abdomen, and raised hips.

[0052] figure 1 Pulsatilla saponin B 4 Effects on the writhing response of mi...

Embodiment 2

[0054] 32 ICR male mice of 19-21g clean grade, certificate number: SCYK ​​(Su) 2013-0003, were randomly divided into formaldehyde model group and B 4 Administration group (50mg / kg). All experimental animals were provided by Zhaoyan (Suzhou) New Drug Research Center Co., Ltd.

[0055] ICR male mice of 19-21 g were acclimated to the laboratory environment for 3 days. Divided into modeling group and B 4 In the administration group (50mg / kg), the mice were put into the observation cage (10×20×15cm) to adapt to the environment for 1 hour before the experiment, and then taken out from the observation cage. Inject 20 μl of 2% formaldehyde solution subcutaneously in the right hind toe of the mouse with a 25 μl micro-syringe, and immediately put it into the observation cage to observe the time and frequency of licking the foot. Produce an obvious spontaneous pain behavior response, that is, the right hind foot shows reactions such as shrinking the leg, licking the paw, and trembling...

Embodiment 3

[0058] 20 clean female SD rats aged 4-6 weeks, certificate number: SCYK ​​(苏) 2013-0003, body weight 180-210g, were randomly divided into 3 groups, 8 blank control groups, complete Freund's adjuvant Induced model group, Pulsatilla saponin B 4 Administration group (6.25mg / kg), 6 rats in each group.

[0059] The treatment group was intraperitoneally injected with Pulsatilla saponin B 2 days in advance 4 , once a day. Modeling was started 1 h after administration on day 0. Except for the subcutaneous injection of 0.1 ml of normal saline in the toes of the control group, the other groups were subcutaneously injected with 0.1 mL of CFA (which contained 10 mg / mL hot water in liquid paraffin) in the right hind foot. Inactivated Mycobacterium tuberculosis); paw swelling, manifested as swelling of the contralateral hindlimb and both forelimb paws, progressive aggravation, nodules and erythema appeared in the ear and tail, suggesting that the Freund's adjuvant induction model was succ...

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Abstract

The invention discloses Pulsatilla saponin B 4 Application in the preparation of medicines for the treatment of pain; the present invention has found that Pulsatilla saponin B 4 It has a good pain-relieving effect on the mouse writhing model induced by acetic acid, the thermal radiation pain model, the formalin-induced mouse pain model and the rat pain model, which can explain that Pulsatilla saponin B 4 It has application value in the treatment of pain.

Description

technical field [0001] The invention belongs to the technology of medicinal use, and in particular relates to Pulsatilla saponin B 4 Application in the preparation of medicines for treating pain. Background technique [0002] Pain is an unpleasant feeling, an emotional experience accompanied by actual or potential tissue damage, and is the most common clinical symptom. It will cause various damages to patients and seriously affect people's quality of life. At present, pain still plagues patients. A frequently asked question. There are many types of pain. According to the location of the pain, it can be divided into superficial pain, deep pain and referred pain, such as pain in the skin and mucous membranes caused by mechanical, chemical, and physical adverse stimuli. Deep pain refers to the pain caused by the stimulation of internal organs, pleura, joints, peritoneum and other parts. Often presents as burning pain without limitation. Referred pain refers to deep pain ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61P29/00A61P19/02A61P19/04
CPCA61K31/704
Inventor 刘艳丽王彦儿康乃馨沈文华李笑然杨世林
Owner SUZHOU UNIV
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