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Preparation method and application of pancreas substrate hydrogel for repairing pancreatic tissue

A technology of hydrogel and pancreas, applied in the field of preparation of pancreatic matrix hydrogel, can solve the problems of large side effects and poor operability of drugs, and achieve the effects of mild implementation conditions, low immunogenicity, and promotion of endogenous repair.

Inactive Publication Date: 2017-09-15
汤礼军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, drugs have been used to regulate the function of macrophages. However, most of the treatments are administered systemically, which has many side effects and poor clinical operability.
However, there are no reports on the preparation of pancreatic matrix hydrogels and their use in pancreatic regeneration.

Method used

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  • Preparation method and application of pancreas substrate hydrogel for repairing pancreatic tissue
  • Preparation method and application of pancreas substrate hydrogel for repairing pancreatic tissue
  • Preparation method and application of pancreas substrate hydrogel for repairing pancreatic tissue

Examples

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Effect test

preparation example Construction

[0053] A method for preparing pancreatic matrix hydrogel for repairing pancreatic tissue, comprising the steps of:

[0054] B. Preparation of porcine pancreatic matrix hydrogel:

[0055] The decellularized pancreas tissue is freeze-dried and made into powder, then mixed with hydrochloric acid-pepsin solution in proportion, and the pH value is adjusted to a physiological state to obtain the porcine pancreas matrix hydrogel.

[0056] Further, the preparation method of the pancreatic matrix hydrogel for repairing pancreatic tissue also includes the steps of:

[0057] A. Decellularization of the pancreas:

[0058] (1) cutting the pancreatic tissue into thin slices, and rinsing with physiological saline to obtain thin slices of pancreatic tissue;

[0059] (2) immersing the thin slice of pancreatic tissue in a physiological saline solution containing sodium lauryl sulfate and stirring to obtain pancreatic tissue;

[0060] (3) The pancreatic tissue is soaked in Triton X-100 soluti...

Embodiment 1

[0063] The preparation of embodiment 1 porcine pancreas matrix hydrogel

[0064] Specific steps are as follows:

[0065] (1) Decellularization of porcine pancreas: first cut porcine pancreas tissue into thin slices about 2 mm thick and rinse with normal saline, then immerse it in 0.5% sodium lauryl sulfate / normal saline, stir at room temperature for 24 hours ;Finally transfer it to 1% Triton X-100 solution, stir at room temperature until the tissue is transparent, it takes about 1 hour, and rinse with normal saline 3-5 times, 15 minutes each time, to remove residual detergent.

[0066] (2) Preparation of porcine pancreatic matrix hydrogel: Grind the decellularized pancreatic tissue freeze-dried into powder with a pulverizer, then add it into hydrochloric acid-pepsin solution at a ratio of 10 mg / mL, stir slowly at room temperature until dissolved, Adjust to the physiological state (pH=7.4) by adding 0.1M sodium hydroxide and 10x PBS, so that the final concentration of the hydr...

Embodiment 2

[0068] Example 2 Use of porcine pancreatic matrix hydrogel in the preparation of drugs that promote endogenous repair of the pancreas

[0069] A. Preparation and identification of porcine pancreatic matrix hydrogel

[0070] (1) Preparation of porcine pancreatic matrix hydrogel: take fresh porcine pancreas, cut off the surface adipose tissue and adventitia, cut the porcine pancreas tissue into thin slices about 2 mm thick and rinse with normal saline several times to remove blood cells, and then Immerse in 0.5% sodium lauryl sulfate / normal saline, stir at room temperature for 24 hours; finally transfer it to 1% Triton X-100 solution, stir at room temperature until the tissue is transparent, about 1 hour, and rinse with normal saline for 3- 5 times, 15 minutes each time, to remove residual detergent. Decellularized pancreases were stored at -80°C.

[0071] (2) Identification of decellularized porcine pancreas: the decellularized porcine pancreas tissue was directly embedded wi...

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Abstract

The invention belongs to the technical field of tissue engineering and regenerative medicines, aims to solve the technical problems that in the prior art pancreas functions are hard to recover and pancreas tissue injury caused by acute necrotizing pancreatitis is hard to recover, and provides a preparation method and application of pancreas substrate hydrogel for repairing pancreatic tissue. The preparation method comprises the following steps: B, preparing pancreas substrate hydrogel, namely performing freeze-drying on decellularized pancreatic tissue, powdering, mixing with a hydrochloric acid-stomach protease solution according to a ratio, and adjusting the pH value to a physiological status, thereby obtaining the pancreas substrate hydrogel. The pancreas substrate hydrogel provided by the invention not only has the advantages of temperature sensitivity, low immunogenicity and capability of providing extracellular microenvironments and the like, but also is simple in operation process, gentle in implementation condition and capable of providing a hydrogel material to pancreas tissue engineering. The material is applicable to growth of pancreas cells, anti-inflammatory M2 type polarization of macrophage can be regulated and controlled, and endogenous repairing of severe pancreatitis pancreatic tissue is promoted.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering and regenerative medicine, and in particular relates to a preparation method and application of a pancreatic matrix hydrogel derived from pig tissue with a function of promoting regeneration and used for repairing pancreatic tissue. Background technique [0002] Severe acute pancreatitis (SAP) is a dangerous disease with rapid progression and many complications, and the fatality rate is as high as 30%. The disease is the pancreatic acinar injury caused by various pathogenic factors, which triggers the release of active pancreatic enzymes into the blood, activates macrophages and neutrophils, and releases a large number of inflammatory factors, leading to systemic inflammatory response and compensatory resistance. The imbalance of inflammatory response, in which macrophage infiltration and activation play a crucial role in the occurrence, development and evolution of SAP. It has been de...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/22A61L27/52
CPCA61L27/227A61L27/3633A61L27/3687A61L27/52A61L2400/06A61L2430/00C08L89/00
Inventor 汤礼军孙红玉陈涛戴睿武黄竹闫洪涛崔建峰梁成霄周菁林宁
Owner 汤礼军
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