Single cell automatic control sorting device and method based on micro-fluidic chip

Abstract

The invention discloses a single cell automatic control sorting device and a single cell automatic control sorting method based on a micro-fluidic chip. The device comprises a cell sorting substrate, a pressure control element, a signal processing element, a channel sorting element and a signal collection control device, wherein the cell sorting substrate comprises the micro-fluidic chip engraved with micro-channels, the micro-channels comprise a main channel provided with a sample liquid storage hole and a waste liquid storage hole arranged at the head and the tail, respectively, and a plurality of cell sorting channels communicating with the main channel and provided with target cell liquid storage holes at tail ends. According to the device and the method provided by the invention, the signal collection control device is used for controlling the channel sorting element to control actions of the element by regulating pressure, so that selection of the cell sorting channel is achieved, and automatic sorting and control of the corresponding cell sorting channel to the target cells are achieved by a negative pressure effect. The device and the method provided by the invention are simple in principle and convenient to use, and the device has the characteristics of small volume and low cost, and can efficiently and conveniently complete sorting of the single cells.

Description

technical field [0001] The present invention relates to the technical field of cell manipulation, in particular to a microfluidic chip-based automatic single cell sorting device and method. Background technique [0002] In many fields such as life sciences, medicine, and biology, it is often necessary to sort out single target cells from more complex samples for subsequent scientific research. For example, in cancer research, sorting and obtaining single cancer cells is a key technology for subsequent cell sequencing. [0003] Currently, the most commonly used single cell sorting technique is to manually isolate single cells from samples with the help of a microscope. This method is time-consuming, difficult to manipulate and prone to damage to cells. Although the cell counting and screening instruments represented by flow cytometry have been integrated and automated, they cannot sort individual cells. [0004] The microfluidic chip is to build a network channel to miniat...

AI Technical Summary

Problems solved by technology

The advantage is that the applied force can control the size, and the cells can be precisely controlled. The disadvantage is that there are many external devices and integration is difficult.

[0012] In summary, using any of the above methods cannot achieve effective single-cell automatic and rapid manipulation, and most of the devices used are complex in structure and inconvenient to operate

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