In-vitro brain-like tissue and establishment method thereof

A technology of brain tissue and tissue structure, used in tissue culture, artificial cell constructs, biochemical equipment and methods, etc.

Inactive Publication Date: 2017-09-15
XI AN JIAOTONG UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The complex structure puts high demands on the construction of brain-like tis

Method used

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  • In-vitro brain-like tissue and establishment method thereof

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Embodiment Construction

[0040] The present invention will be described in further detail below in conjunction with accompanying drawing

[0041] Such as figure 1 As shown, the present invention is an in vitro brain-like tissue. The brain-like tissue structure is constructed by 3D printing. The constructed tissue structure includes cortical layered brain-like tissue 4, functional nuclei brain-like tissue 13 and cortical white matter brain-like tissue 6 Among them, cortical white matter brain-like tissue 6 is close to the structure of cortex 1 and white matter 2 of natural brain tissue in terms of form and function, and cortical layered brain-like tissue 4 is close to the six layers of cortex 1 of natural brain tissue in form and function Organizational structure 3, functional nuclei The brain-like tissue 13 is close to the nuclei 14 structure of natural brain tissue in terms of morphology and function.

[0042] As a preferred embodiment of the present invention, the 3D printed cortical layered brain ...

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Abstract

The invention provides an in-vitro brain-like tissue and an establishment method thereof, and solves the problem of a source of an in-vitro pathological and pharmacological human brain model. According to the establishment method, printing of various nerve cells and cytoskeletons is achieved in a 3D printing way, and single-layer or multi-layer brain-like tissue structures consisting of single cells or multi-cells of human brain cortex tissues, nucleus tissues and cortex and white matter tissues are established; by virtue of own advantages of 3D printing, precise control of the type, the density, the relative position and the like of printed cells can be achieved; through mutual matching of different printing heads, printing of functional nucleus brain-like tissues, cortical stratified brain-like tissues, cortex and white matter brain-like tissues and other multi-layer and multi-gradient structural tissues of various single cells can be achieved; the establishment method is easy to operate and high in efficiency, and is applicable to batch and personalized production; an established brain-like tissue model is closer to a natural brain tissue and provides a basic model for pathological and pharmacological experiments.

Description

Technical field: [0001] The invention belongs to the field of 3D printing and tissue engineering, and in particular relates to an in vitro brain-like tissue and a construction method thereof. Background technique: [0002] Brain science research has been listed as an important strategic direction for the development of science and technology in the world, and it is also one of the most difficult researches to overcome. Especially in the study of brain diseases, the lack of brain tissue donors as the research object limits the development of research, and it is impossible for animal brain tissue to fully characterize the characteristics of human brain tissue. Therefore, the serious shortage of brain pathological and pharmacological models limits the pathological and pharmacological studies of the brain. In addition, the structure of the brain is complex, divided into cortex and white matter, and composed of different nuclei. Macroscopically, the upper cortex covers most of ...

Claims

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Application Information

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IPC IPC(8): C12N5/071B29C64/106B33Y10/00B33Y80/00
CPCC12N5/0697B33Y10/00B33Y80/00C12N2533/50C12N2533/54C12N2533/70C12N2533/72C12N2533/76C12N2533/74C12N2533/10C12N2502/08C12N2502/081C12N2502/088C12N2502/086
Inventor 王玲方奥李涤尘郝志岩
Owner XI AN JIAOTONG UNIV
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