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A three-dimensional printing system and method for heterogeneous cells

A cell printing and three-dimensional printing technology, which is applied in biochemical equipment and methods, biological material sampling methods, biomass post-processing, etc., can solve the problems of inability to complete three-dimensional printing of heterogeneous cells, and achieve the effect of efficient construction

Active Publication Date: 2018-12-28
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the defect that there is no printing device that can combine two different driving printing methods in the prior art, and cannot complete the three-dimensional printing of heterogeneous cells, the present invention provides a printing device that combines two different driving printing methods Heterogeneous Cell 3D Printing System

Method used

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  • A three-dimensional printing system and method for heterogeneous cells
  • A three-dimensional printing system and method for heterogeneous cells
  • A three-dimensional printing system and method for heterogeneous cells

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Embodiment 1

[0039] This embodiment relates to a three-dimensional cell printing system for heterogeneous cells, including a moving platform, an extrusion-type cell printing mechanism and a drop-type cell printing mechanism fixed on the moving platform, an observation unit for observing the cell printing position, and control unit;

[0040] The control unit first controls the extrusion-type cell printing mechanism to complete the printing of the cell hydrogel structure, and then controls the drop-type cell printing structure to complete the printing of a single cell on the cell hydrogel structure; during the printing process, The control unit controls the motion platform to move in the three directions of X, Y, and Z, so as to realize printing at different positions of the extruding cell printing mechanism and the dropping cell printing mechanism.

Embodiment 2

[0042] This embodiment relates to a 3D printing system for heterogeneous cells, the structure of which is as follows figure 1 and figure 2 Compared with Embodiment 1, the difference is that the printing system is placed in the molding chamber 1, and an ultraviolet sterilization lamp 14 is arranged in the molding chamber;

[0043] The extrusion-type cell printing mechanism is fixed on the Z-axis motion platform 2 of the motion platform, and consists of a syringe drive motor 10, a heat preservation sleeve 11, a 1mL syringe and its nozzle 12; the drop-type cell printing mechanism is fine-tuned by position A motor 20, an indenter 21, a piezoelectric ceramic 4, and a drop-type cell printing micro-spray head 7 are all fixed on the Z-axis motion platform 2 through the print head clamping mechanism 3;

[0044] The observation unit includes a horizontal observation microscope 5 arranged on the nozzle clamping mechanism 3 and a vertical observation microscope 15 arranged at the bottom...

Embodiment 3

[0048] This embodiment relates to a method for printing a tumor cell model using the heterogeneous cell three-dimensional printing system of the present invention, which is characterized in that it includes the following steps:

[0049] 1) Prepare cell solution for extrusion cell printing. The culture solution containing A549 cells was centrifuged and suspended in the culture medium to reach 4×10 6 / ml cell density. Afterwards, the A549 cell culture solution, 4% sodium alginate solution and 20% gelatin solution were mixed according to the volume ratio of 1:1:1, and the final cell density was 1×10 6 / ml or so.

[0050] 2) Prepare the cell solution for drop cell printing. The A549 cell culture solution was aspirated and diluted so that the final cell density was 5×10 4 / ml. Among them, it is worth noting that the cell density in the cell solution can be adjusted according to the needs of different models, and is not limited to the currently used cell density.

[0051] 3) T...

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Abstract

The present invention relates to a heterogeneous cell three-dimensional printing system, which comprises a moving platform, an extruded cell printing mechanism fixed on the moving platform, a dropping-off type cell printing mechanism fixed on the moving platform, an observation unit for observing and feeding back the cell printing condition, and a control unit, wherein the control unit controls the extruded cell printing mechanism to complete the three-dimensional cell hydrogel structure printing, and then controls the dropping-off type cell printing mechanism to complete the printing of single or multiple cells on the three-dimensional cell hydrogel structure. According to the present invention, the micro-extrusion type printing technology and the dropping-off type cell printing technology based on the alternating hysteresis force driving are integrated, such that the efficient construction of the macroscopical cell clusters is ensured, the construction of the microenvironment of the microcosmic single cell near the cluster cells is ensured, the in vitro heterogeneous cell model construction method integrating the efficiency and the precision is created, and the system and the method have important pathology and pharmacology application potential.

Description

technical field [0001] The invention belongs to the fields of biomanufacturing and cell printing, and in particular relates to a precise and controlled assembly device and process for heterogeneous cells. Background technique [0002] In recent years, cell printing technology has played an increasingly important role in the fields of tissue engineering, pathological model construction, drug screening and detection, and cell behavior research. In the field of tissue engineering, cell printing technology realizes the three-dimensional controlled assembly of heterogeneous cells, and can construct complex tissue structures in vitro, which promotes the development of tissue engineering in the direction of scale, automation, and computer-aided processing; in pathological models In the field of construction, cell printing can assemble diseased cells (such as tumor cells) and normal cells in a specific order, which provides an effective research method for medical workers to study t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36B33Y30/00
CPCC12M33/04C12M41/00
Inventor 孙伟姚睿刘天坤赵龙
Owner TSINGHUA UNIV
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