Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A TLC identification method for Danlv Bushen Capsules

A technology of kidney-tonifying capsules and thin-layer chromatography, applied in the field of drug analysis, can solve the problems of complicated identification process, complicated preparation process, reagent toxicity, etc., and achieve the effect of good repeatability, simple preparation process and clear spots

Active Publication Date: 2019-02-05
SHINEWAY PHARMA GRP LTD +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a TLC identification method for Danlv Bushen Capsules, aiming to solve the problems of complex preparation process of the test solution, high toxicity of reagents used, and complicated and cumbersome identification process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A TLC identification method for Danlv Bushen Capsules
  • A TLC identification method for Danlv Bushen Capsules
  • A TLC identification method for Danlv Bushen Capsules

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0075] Preparation of the test solution: take 1 g of the content of this product, add 5 mL of methanol, ultrasonically extract for 30 min, filter, and the filtrate is the test solution of plumbago.

[0076] Preparation of the reference solution: Take 1 g of plumbago as reference medicinal material, add 5 mL of methanol, ultrasonically extract for 30 min, filter, and the filtrate is the plumbago reference solution.

[0077] Negative sample solution preparation: according to the prescription ratio, take other medicinal flavors except plumbago to make capsules according to the process, and then prepare plumbago negative sample solution according to the above-mentioned preparation method of the test solution.

[0078] TLC conditions: According to the TLC (Chinese Pharmacopoeia 2015 Edition Sibu General Rules 0502) test, draw 5 μL of each of the above solutions, and spot them on the same silica gel G TLC plate, and use petroleum ether with a volume ratio of 8:4:1 (30-60°C)-chlorofo...

Embodiment 1

[0137] Preparation of Danlv Bushen Capsules

[0138] The Danlv Bushen Capsules are composed of the following raw materials in parts by weight: Plumbago dan: 2-4 parts, Lvbaoteng: 8-12 parts, Shegan: 8-12 parts, pepper: 1-3 parts, dried ginger : 2-4 servings.

[0139] The Danlv Bushen Capsules are prepared according to the following method: in parts by weight, weigh the five herbs of Plumbago, Lvbaoteng, Shegan, Pepper, and Dried Ginger; Use ethanol as a solvent, immerse for 48 hours and percolate, collect the filtrate, recycle the ethanol and concentrate it into a clear paste; slice the dried ginger, and extract the volatile oil by steam distillation; crush plumbago and pepper into coarse powder, mix well, add ethanol to extract three times, Filtrate, combine the filtrates, recover ethanol and concentrate into a clear paste, combine the above two clear pastes, concentrate on a water bath to form a thick paste, dry, mix with ginger oil adsorbed by starch, crush, sieve, mix wel...

Embodiment 2

[0142] The TLC identification method for the Danlv Bushen Capsules prepared in Example 1 includes the TLC identification of Plumbania, Lvbaoteng, Shegan, and Pepper.

[0143] Take 1g of the contents of Danlv Bushen Capsules, add 5mL of methanol, ultrasonically extract for 15min, filter, and the filtrate is the test solution containing Plumbago, Lvbaoteng, Shegan, and pepper.

[0144]The TLC identification of plumbago includes at least the following steps:

[0145] Step 1. Take 1 g of plumbago as reference medicinal material, add 5 mL of methanol, ultrasonically extract for 15 minutes, filter, and the filtrate is the plumbago reference solution;

[0146] Step 2, test according to thin-layer chromatography (Chinese Pharmacopoeia 2015 edition four general rules 0502), respectively draw plumbago for the test and plumbago for reference substance solution 10 μ L, point respectively on the same silica gel G thin-layer plate, with the volume ratio of 6:3:0.5 petroleum ether (30-60°C)...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a thin-layer chromatographic identification method for a Radix Plumbaginis Zeylanicae-Tinospora crispa capsule capable of tonifying kidney, belonging to the technical field of pharmaceutical analysis. The method comprises the following steps: separately preparing a test solution containing Radix Plumbaginis Zeylanicae, Tinospora crispa, blackberry lily rhizomes and pepper and control solutions of Radix Plumbaginis Zeylanicae, Tinospora crispa, blackberry lily rhizomes and pepper through ultrasonic extraction with methanol; then carrying out thin-layer chromatographic identification on Radix Plumbaginis Zeylanicae, Tinospora crispa, blackberry lily rhizomes and pepper by using different developing agents; and determining whether fluorescence spots with same colors occur in both the color spectra of test substances and the color spectra of control substances at corresponding positions. According to the thin-layer chromatographic identification method provided by the invention, the test solution and the control solutions are easy to prepare; reagents used in the method are low in toxicity and usage amount; the method is simple and fast; the spots are clear; good repeatability is obtained; and negative control poses no interference.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and more specifically relates to a TLC identification method for Danlv Bushen Capsules. Background technique [0002] In terms of disease prevention and treatment, traditional Chinese medicine compound extraction preparations occupy an important position. After the traditional Chinese medicine is extracted, the plant tissues are no longer consistent, and microscopic identification is powerless. Therefore, the quality control methods are based on various active ingredients. TLC identification and content determination. However, in various national quality standards, TLC identification mostly deals with a sample solution, a TLC board, unfolded once, and a chromogen to identify a single medicinal material. In order to eliminate interference, the sample pretreatment procedures are complex and cumbersome, requiring repeated purification with a large amount of organic reagents, which is laboriou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 周永妍姜国志王文鹏李龙龙屈云萍李菲孙胜斌陈钟李振江
Owner SHINEWAY PHARMA GRP LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com