Preparation method and application of herba ardisiae japonicae alcohol extract

A technology of tea alcohol and tea alcohol, which is applied in the field of preparation of tea alcohol extract, achieves significant inhibitory effect and increased mRNA expression level

Inactive Publication Date: 2017-09-22
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Preparation method and application of herba ardisiae japonicae alcohol extract

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[0022] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0023] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0024] Such as figure 1As shown, the preparation method of the ethanol extract provided by the embodiments of the present invention comprises the following steps:

[0025] S101: Preparation of Alcoholic Extract of C. chinensis: after drying and pulverizing the medicinal material of C. chinensis at low temperature, accurately weigh 0.1 g, add 0.1 mL of methanol, vortex and oscillate for 60 minutes, and then ultrasonicate for 60 minutes at a power of 200 W and a frequency of 40 kHz. Al...

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and discloses a preparation method and application of a herba ardisiae japonicae alcohol extract. The preparation method comprises the following steps: after drying and crushing a herba ardisiae japonicae medicinal material at low temperature, weighing 0.1g of the crushed herba ardisiae japonicae medicinal material, and adding 0.1mL of methanol; after carrying out vortex oscillation, carrying out ultrasonic treatment and cooling; then weighing the weight, and supplementing the reduced weight with the methanol; uniformly shaking and filtering; taking subsequent filtrate and preserving for later use; weighing a bergenin reference substance, and adding the methanol to prepare a solution; determining the content of bergenin in the herba ardisiae japonicae alcohol extract by adopting an ultra-high performance liquid chromatography. According to the preparation method and the application of the herba ardisiae japonicae alcohol extract, the content of the bergenin in the herba ardisiae japonicae alcohol extract is 9.88mg/g and accords with quality standards; herba ardisiae japonicae can be used for reducing the quantity of lipid droplets in ST-2 and C3H10 cells which are obtained by inducing adipogenesis differentiation, and for inhibiting mRNA expression of adipogenesis differentiation characterization genes PPARgamma, C/EBPalpha and aP2; interventional treatment of the herba ardisiae japonicae can be used for effectively reducing the weight of C57BL/6 mice fed by high fat diet.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a preparation method and application of an alcoholic extract of tealeaves. Background technique [0002] Obesity (OB) is a chronic metabolic disease characterized by an increase in the number and volume of adipocytes (AD) in the body, resulting in an abnormally high percentage of body fat, accompanied by excessive deposition of local adipose tissue. In recent years, with the improvement of socioeconomic level, obesity is spreading at an alarming rate among adults and adolescents all over the world, especially the trend of obesity occurring at a younger age is particularly prominent. Obesity not only reduces the quality of life of patients, but also is a common risk factor for many diseases such as diabetes, coronary heart disease, atherosclerosis, hypertension, hyperlipidemia, and tumors. The impact of obesity on human health has become a global ...

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Application Information

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IPC IPC(8): A61K36/185A61P3/04C12N5/077C12Q1/02
CPCA61K36/185A61K2236/15A61K2236/33C12N5/0653C12N2500/76C12N2506/1353G01N33/5008G01N33/5073
Inventor 郑纺崔壮常艳旭王宝利王海晓陈莹
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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