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Recombinant rat phospholipase Cgamma2 adenovirus as well as construction method and application thereof

A rat phospholipid and adenovirus technology, applied in applications, viruses, viruses/phages, etc., can solve the problems of long-term stable expression of foreign genes, weak specificity of target genes, and inability to be effectively regulated, and achieve prevention and treatment. Liver tumors, promoting apoptosis and regulating the proliferation of liver cancer cells

Inactive Publication Date: 2017-09-29
HENAN UNIV OF SCI & TECH
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Problems solved by technology

[0007] The purpose of the present invention is to provide a recombinant rat phospholipase Cγ2 adenovirus, its construction method and its application, which solves the problem of weak target gene specificity and expression in liver cancer cells in the clinical application of gene diagnosis and treatment of existing liver cancer. The positive rate is low, and the exogenous gene introduced into the body during gene therapy cannot be stably expressed in the cell for a long time and cannot be effectively regulated

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  • Recombinant rat phospholipase Cgamma2 adenovirus as well as construction method and application thereof
  • Recombinant rat phospholipase Cgamma2 adenovirus as well as construction method and application thereof
  • Recombinant rat phospholipase Cgamma2 adenovirus as well as construction method and application thereof

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Embodiment Construction

[0046] The present invention will be described in detail below with reference to the drawings and specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. The test methods for which specific conditions are not indicated in the following examples are usually operated under conventional conditions, and the steps are not described in detail because they do not involve the invention point.

[0047] When the examples give numerical ranges, it should be understood that, unless otherwise stated in the present invention, the two endpoints of each numerical range and any value between the two endpoints can be selected. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, equipment, and materials used in the embodiments, according to those skilled in the art's gra...

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Abstract

The invention discloses a recombinant rat phospholipase Cγ2 adenovirus, which is obtained by inserting the rat phospholipase Cγ2 gene into an adenovirus shuttle plasmid pHBAd‑MCMV‑GFP containing the adenovirus genome, the rat phospholipase Cγ2 gene The nucleotide sequence is shown in SEQ ID NO.1. The present invention also provides the construction method and application of the above-mentioned recombinant rat phospholipase Cγ2 adenovirus. Aiming at the promoting effect of PLCγ2 in cancer cell apoptosis, the present invention constructs a PLCγ2 recombinant adenovirus to replace the gene model, which can regulate the growth of cancer cells in a targeted manner, and the recombinant rat phospholipase Cγ2 adenovirus can stably express rat phospholipids Enzyme Cγ2, with high virus titer and significant pro-apoptotic effect; recombinant adenovirus and the rat phospholipase Cγ2 expressed by it can significantly inhibit cell proliferation and promote apoptosis of rat liver tumor cells, and can be used for the prevention of liver tumors And treatment provides new treatment ideas.

Description

technical field [0001] The invention belongs to the field of biological virus construction, and specifically relates to a recombinant rat phospholipase Cγ2 adenovirus, a construction method and application thereof. Background technique [0002] The liver is an important organ of the body. It has exocrine, endocrine, metabolism, and biotransformation functions. It participates in the metabolism, storage, and redistribution of nutrients such as sugar, fat, and vitamins, and plays an important role in maintaining the body's metabolic balance. The liver is also the main detoxification organ of the body, removing metabolic waste and foreign substances through biotransformation and bile excretion. Due to the many important physiological functions of the liver, various liver diseases will occur when the liver function is out of balance. There are hundreds of related diseases, the common ones are liver tumor, fatty liver, liver fibrosis, acute liver necrosis, and toxic liver diseas...

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Application Information

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IPC IPC(8): C12N15/861A61K48/00A61P35/00
CPCC12N15/86A61K48/005C12N9/16C12N2710/10043C12Y301/04003
Inventor 陈晓光刘玉梅吕琼霞张自强邓雯
Owner HENAN UNIV OF SCI & TECH
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