Bacillus velezensis strain JN5 and application thereof

A technology of Bacillus Velez and Bacillus, applied in the field of microorganisms, can solve problems such as economic losses, drug residues, and control difficulties, and achieve the effects of reducing usage and residues, promoting flowering and fruiting, and reducing production costs

Inactive Publication Date: 2017-10-17
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prevalence of rice streak disease has caused significant economic losses to agricultural production, forcing people to continuously increase investment in research on its control
But disease prevention and control still faces many problems: lack of vertical disease-resistant varieties in production
Only a small number of rice varieties in my country show quantitative resistance to streak disease, and less than 10% of the rice varieties in Yunnan show disease resistance to streak disease. The emergence of drug-resistant strains makes control extremely difficult
[0004] The application of chemical agents to preve

Method used

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  • Bacillus velezensis strain JN5 and application thereof
  • Bacillus velezensis strain JN5 and application thereof
  • Bacillus velezensis strain JN5 and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0029] The Bacillus velez strain JN5 is prepared as a liquid, and the preparation method includes the following steps:

[0030] (1) Bacteria culture

[0031] a. Test tube seed culture: Sterilize NA medium at 121°C for 30 minutes and pour it into a 9cm plate to make a NA medium bottom plate, inoculate Bacillus Velez JN5, and cultivate at 28°C for 24-48h;

[0032] The formula of the NA medium is: 10 g glucose, 5 g peptone, 3 g beef extract, 1 g yeast extract, 17 g agar powder, and make up to 1000 mL with distilled water;

[0033] b. Bacterial shaker expansion culture: inoculate the plate seeds on the NA medium on the slant surface of the eggplant bottle and cultivate at 28°C for 24-48h to obtain the eggplant bottle seeds;

[0034] (2) Fermentation

[0035] a. Preparation of seed suspension: Wash the colonies on the slope of the eggplant bottle with sterilized water to obtain a seed suspension;

[0036] b. Inoculation: Pass the seed suspension through the inoculation port and use the pressur...

Embodiment 1

[0046] Preparation of Bacillus velezensis JN5 liquid

[0047] (1) Bacteria culture

[0048] a. Test tube seed culture (the following are weight percentages)

[0049] Put NA medium (peptone 5g, sucrose 10g, yeast powder 1g, beef extract 3g, agar 17-20g) into a test tube, make up to 1000 mL with distilled water, sterilize, make a plate, and inoculate Velez Bacillus JN5 Strains were cultured at 28°C for 48h.

[0050] b. Expansion of culture in a shaker

[0051] The plate seeds are inoculated on the eggplant bottle slope NA medium (the medium formula is the same as in the above step a), and cultivated at 26°C for 48 hours to obtain eggplant bottle seeds.

[0052] (2) Fermentation

[0053] The specific fermentation production process is as follows:

[0054] a. Preparation of seed suspension: Wash the colonies on the slope of the eggplant bottle with sterilized water to obtain a seed suspension;

[0055] b. Inoculation: Pass the seed suspension through the inoculation port and use the pressure d...

Embodiment 2

[0060] Preparation of Bacillus velezensis JN5 coating agent

[0061] (1) Preparation of liquid biocontrol bacteria: streak culture of biocontrol bacteria on NA medium described in Example 1, inverted culture at 28°C for 48h, pick a single colony to inoculate in NA liquid medium, culture at 28°C at 160rpm with shaking for 48h , Adjust the OD of bacterial suspension under UV spectrophotometer 600 nm=0.5, that is, the concentration of the bacterial suspension is equivalent to 1X10 8 CFU / mL is reserved.

[0062] (2) Coating agent formula: 4g polyvinyl alcohol dissolved in 96mL ddH 2 Prepare 4% polyvinyl alcohol solution in O; 4g chitosan is dissolved in 96mL ddH2O to prepare 4% chitosan solution; 2g xanthan gum is dissolved in 98mL ddH2O to prepare 2% xanthan gum solution. According to the volume of 4% polyvinyl alcohol: 4% chitosan: 2% xanthan gum=4:1:1, prepare a mixed solution, and then prepare a coating agent according to the mixed solution: biocontrol bacteria suspension=100:1 . ...

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Abstract

The invention discloses a bacillus velezensis strain JN5 and an application thereof. The bacillus velezensis strain JN5 is registered in CGMCC on 13th, December, 2016, the preservation number being CGMCC No.13455. The bacillus velezensis strain JN5 provided by the invention can be prepared into an aqueous agent or a coating agent to be acted on crops such as corn and rice for preventing bacterial stripe of rice and bacterial blight of rice and promoting growth of corn and rice. The strain is safe to use and free of residues, the dosage and residual amount of a chemical agent in an agricultural product are favorably reduced, the production cost is lowered, and the strain is high in practicality and suitable for industrial production.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and particularly relates to a Velez Bacillus strain JN5 and its application. Background technique [0002] Corn is the most widely distributed food crop in Yunnan Province with the largest planting area. It includes fodder corn, sweet corn, waxy corn, etc. It is very widely distributed and can be divided into four planting areas in Yunnan: cold corn area in northwestern Yunnan, warm in central Yunnan Corn area, warm and hot corn area in southern Yunnan, and warm and cool corn area in northeast Yunnan. In recent years, the planting area of ​​sweet corn in our province has gradually expanded, and now it has become a key development pillar industry with plateau characteristics. The scope of corn cultivation in our country is wide. In different corn ecological areas, the occurrence of corn diseases is not exactly the same due to the influence of various factors such as the varieties planted in var...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00A01P21/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 姬广海魏兰芳朱书生付丽娜杨俊汪娅婷王星
Owner YUNNAN AGRICULTURAL UNIVERSITY
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