Compound medicine composition with lung cancer resistant effect and application of compound medicine composition
A composition and anti-lung cancer technology are applied in the field of natural medicines to achieve the effects of reducing toxic and side effects, developing good clinical drug development prospects, and strong anti-proliferative activity
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Embodiment 1
[0018] Example 1: Screening and optimizing the compound pharmaceutical composition of perylene amide (PLM) and emodin (ED) in lung cancer cells.
[0019] Lung cancer cells in the logarithmic growth phase were inoculated with 3×10 4 Cells / well were placed on a 96-well plate, and after 6 hours of growth, the supernatant was discarded by centrifugation, and then administered according to the following groups: Tumor cells were divided into no drug group and drug-added group, and the drug-added group consisted of ED and PLM single drugs Group, ED and PLM combined drug groups with different molar ratios, set 4-6 duplicate wells in each group, culture for 24 hours, discard the supernatant, add 100 μl of MTT (tetrazolium salt) serum-free culture medium containing 0.5 mg / ml for culture After 4 hours, add 100 μl of DMSO (dimethyl sulfoxide), place on a micro-oscillator to vibrate for 10 minutes, and then place on a microplate reader to detect the OD value at 570 nm. Results The inhibit...
Embodiment 2
[0025] Example 2: Analysis of the combined administration effect of PLM and ED
[0026] Based on the Median-effect Principle (moderate effect principle or Chou-Talalay combined index method), the dose-response curve and the combined index curve (fa-C curve) under different effects were drawn using CombiDrug statistical software. The relationship between the effect and the combination index quantitatively evaluates whether there is a synergistic, antagonistic or additive relationship between the two drugs. Specific steps are as follows:
[0027] The drug effect is the inhibition rate (fa)=1-(the average OD570 value of the test group / the average OD570 of the tumor cell blank control group) according to the intermediate effect equation fa / fu=(D / Dm) m , take the logarithm logfa / fu=mlogD–mlogDm on both sides, set a=-mlogDm, b=m, x=logD, y=logfa / fu, and substitute into the intermediate effect equation to get y=bx–a; where fa is the drug effect , fu=1–fa, D is the drug concentratio...
Embodiment 3
[0031] Embodiment 3: Pilongamide (PLM) and emodin (ED) compound pharmaceutical composition are made tablet according to following prescription:
[0032] The prescription is as follows:
[0033]
[0034] The preparation process is as follows:
[0035] Pass the medicine and auxiliary materials through a 80-mesh sieve respectively, fully mix the perylene amide with 50 grams of microcrystalline cellulose and 12 grams of carboxymethyl starch sodium, make soft materials with 10% starch slurry, granulate with a 18-mesh sieve, and dry at 60°C , get particle 1. Fully mix emodin with 12 grams of microcrystalline cellulose, 15 grams of starch and 8 grams of sodium carboxymethyl starch, make soft material with 10% starch slurry, granulate with a 18-mesh sieve, and dry at 60°C to obtain Granule 2. According to the principle of equal increase, fully mix granule 1 and granule 2, sieve the granules with 16 mesh, add magnesium stearate, mix well, and compress into tablets, the tablet weight...
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