Application of baicalein to preparation of drug for preventing and/or treating nasopharyngeal carcinoma

A technology of baicalein and nasopharyngeal cancer, applied in the field of baicalein, can solve problems such as difficult to effectively prevent and treat, and achieve the effect of reducing incidence and mortality and blocking transformation

Inactive Publication Date: 2017-11-10
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Due to the multi-level complexity of human organs, tissues, cells, and molecules, especially for cancers caused by viruses, the relationship between the viral tumor protein produced by the virus and the proliferation of human cancer cells is often difficult to effectively prevent and treat by conventional means , so it is very meaningful to seek drugs that can effectively inhibit tumors by acting on the viral tumor protein produced by the virus

Method used

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  • Application of baicalein to preparation of drug for preventing and/or treating nasopharyngeal carcinoma
  • Application of baicalein to preparation of drug for preventing and/or treating nasopharyngeal carcinoma
  • Application of baicalein to preparation of drug for preventing and/or treating nasopharyngeal carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] [Example 1] The effect of baicalein on tumor cell proliferation.

[0077] Experimental protocol: Cell Counting Kit-8 (CCK-8) experiment was used, nasopharyngeal carcinoma cells HONE1 / Akata and HK1 / Akata were treated with baicalein for about 24, 48, and 72 hours, and 10 μl of CCK-8 reagent was added to each well, protected from light Place in a 37°C incubator and incubate for 1 h, and measure the absorbance at 450 nm with a microplate reader. The cell survival rate was calculated according to the following formula: cell survival rate=(OD experimental well-OD blank well) / (OD control well-OD blank well)×100%.

[0078] Experimental results: For specific results of the CCK-8 experiment, see figure 1 , indicating that baicalein has a significant inhibitory effect on the proliferation of nasopharyngeal carcinoma cells HONE1 / Akata and HK1 / Akata. Data are presented in Table 1A and Table 1B.

[0079] Table 1A: Survival rate of nasopharyngeal carcinoma cell line HONE1 / Akata tre...

Embodiment 2

[0083] [Example 2] The effect of baicalein on the transcription and translation levels of Epstein-Barr virus nuclear antigen 1.

[0084] Experimental program:

[0085] The effect of baicalein on the transcription level of EBNA1 detected by SYBR green fluorescent real-time quantitative PCR method: different concentrations of baicalein were used to treat nasopharyngeal carcinoma positive cell lines HONE1 / Akata and HK1 / Akata, and cervical cancer cells HeLa48 that temporarily overexpress EBNA1 After 24 hours, total cellular RNA was extracted after treatment for 24 hours, and its concentration and purity were determined by NanoDrop 2000 (A260 / A280ratio>1.8). 1 μg of total RNA was used to obtain cDNA using TAKARA reverse transcription kit. It is carried out in two steps, the first step is to remove the genomic DNA mixed in the extracted total RNA, and the second step is to reverse transcribe the extracted RNA to make it into cDNA. The cDNA obtained by the above reverse transcriptio...

Embodiment 3

[0097] [Example 3] Temporarily increasing the expression of EBNA1 in vitro can alleviate the reduction of the activity of baicalein on tumor cells.

[0098] Experimental scheme: Transfect plasmids that can temporarily express EBNA1 protein in vitro in nasopharyngeal carcinoma cells HONE1 / Akata and HK1 / Akata, and use Western blot to detect whether the expression of EBNA1 is confirmed to increase, and then use the CCK-8 kit Whether the increase of EBNA1 expression has an effect on the inhibitory effect of baicalein on tumor cell activity was examined.

[0099]Experimental results: WB results show that the temporary expression of EBNA1 in vitro can alleviate the inhibition of baicalein on the expression level of EBNA1 (results see image 3 , see Table 3A for data), and attenuated the reduction of baicalein's activity on tumor cells (see 3B and 3C for data).

[0100] Table 3A: Quantitative analysis of the effect of baicalein on EBNA1 protein expression in nasopharyngeal carcinoma...

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Abstract

The invention discloses an application of baicalein to the preparation of a drug for preventing and / or treating nasopharyngeal carcinoma. The application firstly proves that baicalein can remarkably inhibit a viral protein EBNA1 on which an Epstein-Barr virus exists, and a cell can become a normal cell due to tumor virus loss caused by overlow existing quantity of the protein in the cell. Baicalein can be favorably used for treating precancerous lesion, the conversion of severe rhinitis to nasopharyngeal carcinoma is effectively stopped, and the application has important significance for treating the precancerous lesion of nasopharyngeal carcinoma and reducing the occurrence rate and fatality rate of nasopharyngeal carcinoma.

Description

technical field [0001] The present invention relates to the application of baicalein, in particular to the application of baicalein in the preparation of drugs for preventing and / or treating nasopharyngeal carcinoma. Background technique [0002] Tumor is the human body under the action of various carcinogenic factors, the molecules are destroyed, so the cells lose the regulation of their normal growth at the gene level, resulting in the disordered abnormal proliferation of the cells and the formation of abnormal tissues. The abnormal tissues continue to grow and will break through the blood vessel wall Thereby gaining nutrients and growing rapidly, manifested as lumps, thereby destroying the functions of human organs. According to valid statistics, tumor is one of the two major factors that threaten human life and health all over the world. Among them, nasopharyngeal carcinoma (nasopharyngeal carcinoma, NPC) is a malignant tumor of the head and neck originating from the ep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/00A61P31/22
CPCA61K31/352
Inventor 孙晓平张雅倩王超
Owner WUHAN UNIV
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