Application of baicalein to preparation of drug for preventing and/or treating nasopharyngeal carcinoma
A technology of baicalein and nasopharyngeal cancer, applied in the field of baicalein, can solve problems such as difficult to effectively prevent and treat, and achieve the effect of reducing incidence and mortality and blocking transformation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0076] [Example 1] The effect of baicalein on tumor cell proliferation.
[0077] Experimental protocol: Cell Counting Kit-8 (CCK-8) experiment was used, nasopharyngeal carcinoma cells HONE1 / Akata and HK1 / Akata were treated with baicalein for about 24, 48, and 72 hours, and 10 μl of CCK-8 reagent was added to each well, protected from light Place in a 37°C incubator and incubate for 1 h, and measure the absorbance at 450 nm with a microplate reader. The cell survival rate was calculated according to the following formula: cell survival rate=(OD experimental well-OD blank well) / (OD control well-OD blank well)×100%.
[0078] Experimental results: For specific results of the CCK-8 experiment, see figure 1 , indicating that baicalein has a significant inhibitory effect on the proliferation of nasopharyngeal carcinoma cells HONE1 / Akata and HK1 / Akata. Data are presented in Table 1A and Table 1B.
[0079] Table 1A: Survival rate of nasopharyngeal carcinoma cell line HONE1 / Akata tre...
Embodiment 2
[0083] [Example 2] The effect of baicalein on the transcription and translation levels of Epstein-Barr virus nuclear antigen 1.
[0084] Experimental program:
[0085] The effect of baicalein on the transcription level of EBNA1 detected by SYBR green fluorescent real-time quantitative PCR method: different concentrations of baicalein were used to treat nasopharyngeal carcinoma positive cell lines HONE1 / Akata and HK1 / Akata, and cervical cancer cells HeLa48 that temporarily overexpress EBNA1 After 24 hours, total cellular RNA was extracted after treatment for 24 hours, and its concentration and purity were determined by NanoDrop 2000 (A260 / A280ratio>1.8). 1 μg of total RNA was used to obtain cDNA using TAKARA reverse transcription kit. It is carried out in two steps, the first step is to remove the genomic DNA mixed in the extracted total RNA, and the second step is to reverse transcribe the extracted RNA to make it into cDNA. The cDNA obtained by the above reverse transcriptio...
Embodiment 3
[0097] [Example 3] Temporarily increasing the expression of EBNA1 in vitro can alleviate the reduction of the activity of baicalein on tumor cells.
[0098] Experimental scheme: Transfect plasmids that can temporarily express EBNA1 protein in vitro in nasopharyngeal carcinoma cells HONE1 / Akata and HK1 / Akata, and use Western blot to detect whether the expression of EBNA1 is confirmed to increase, and then use the CCK-8 kit Whether the increase of EBNA1 expression has an effect on the inhibitory effect of baicalein on tumor cell activity was examined.
[0099]Experimental results: WB results show that the temporary expression of EBNA1 in vitro can alleviate the inhibition of baicalein on the expression level of EBNA1 (results see image 3 , see Table 3A for data), and attenuated the reduction of baicalein's activity on tumor cells (see 3B and 3C for data).
[0100] Table 3A: Quantitative analysis of the effect of baicalein on EBNA1 protein expression in nasopharyngeal carcinoma...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com