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Method for increasing content of grease and fatty acid in tribonema

A technology for oil content and yellow silk algae, applied in the field of biotechnology or food, can solve problems such as affecting the growth and accumulation of algal biomass, and achieve the effects of reducing composition content, improving fatty acid content, and simple operation

Active Publication Date: 2017-11-17
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that algae can increase the oil content of algae under the condition of nitrogen deficiency or other conditions, but this will seriously affect the growth accumulation of algae biomass

Method used

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  • Method for increasing content of grease and fatty acid in tribonema
  • Method for increasing content of grease and fatty acid in tribonema

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Pour the prepared fresh BG11 medium into the light column reactor, and insert the logarithmic phase of Xanthophyllum, the inoculum amount is 10%. Then Rhizobium rosette ( Rhizobium rosetiformans ) cultured for 1d-10d, washed with sterile water and inserted into Rhizobium rosette ( Rhizobium rosetiformans , R group), the inoculum volume was 1%; the blank group (CK group) was not inoculated with bacteria. Place in a climate incubator, set the temperature at 28°C, light for 24 hours, light intensity 40%, and directly pass into the environment mixed with 5% CO 2 air, the ventilation volume is about 0.5vvm. Such as figure 1 As shown in Table 1, after culturing for one week, in the experimental group in which the bacteria were inserted, the oil content was 53% of the dry weight of the yellow silk algae, and the oil content was higher than that of the CK group (43%). The oil accumulation of algae has a promoting effect, and the amount of oil accumulation has increased b...

Embodiment 2

[0023] Pour the prepared fresh BG11 culture medium into the light column reactor, and insert the Zytophthora algae, the inoculum amount is 10%. Hydrogenophage xanthus ( Hydrogenophaga intermedia ) cultured for 1d-10d, washed with sterile water and inserted into the yellow hydrogen phagocytosis ( Hydrogenophaga intermedia ) (Group H), the inoculation volume was 1%. Blank group (CK group), no bacteria were inserted. Place in a climate incubator, set the temperature at 28°C, light for 24 hours, light intensity 40%, and directly pass into the environment mixed with 5% CO 2 air, the ventilation volume is about 0.5vvm. Such as figure 1 As shown in Table 1, after culturing for one week, in the experimental group inoculated with bacteria, the oil content was 45.67% of the dry weight of Xanthophyllum, and the oil content was higher than that of the CK group (43%). Oil accumulation has a promoting effect. The accumulation of saturated fatty acids (Saturated fatty acids, SFA) in...

Embodiment 3

[0025] Pour the prepared fresh BG11 medium into the light column reactor, and insert the logarithmic phase of Xanthophyllum, the inoculum amount is 10%. Then the sphingosine bacteria ( Sphingopyxis ummariensis ) cultured for 1d-10d, washed with sterile water and inserted into the sphingosine box bacteria ( Sphingopyxis ummariensis , S group), the inoculation volume was 1%. The blank group (CK group) was not inoculated with bacteria. Place in a climate incubator, set the temperature at 28°C, light for 24 hours, light intensity 40%, and directly pass into the environment mixed with 5% CO 2 air, the ventilation volume is about 0.5vvm. Such as figure 1 As shown in Table 1, after one week of culture, the oil content was 48.5% of the dry weight of Xanthophylla, and the oil content was higher than that of the CK group (43%). The effect of oil accumulation increased by 12.79% was more significant. The accumulation of saturated fatty acids (Saturated fatty acids, SFA) in Xanth...

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Abstract

The invention relates to a method for increasing the content of grease and fatty acid in tribonema and belongs to the field of biotechnology and food engineering. The method is specifically the biological technology for increasing the content of grease in tribonema and influencing the composition of fatty acid by adding certain bacteria to a culturing system. The prepared oil-enriched microalga can be used as a raw material for production of vegetable fat and development of polyunsaturated fatty acid feed additives.

Description

technical field [0001] The invention relates to a method for increasing oil content and fatty acid composition of algae, which belongs to the field of biotechnology or food technology. Background technique [0002] Although there are many kinds of microalgae, different species accumulate different metabolites. Under normal growth conditions, the oil content of most microalgae cells is generally 10-30%, which is far from meeting the needs of biofuels and high value-added food (feeding). The demand for the production of oil additives and the limited level of large-scale cultivation of microalgae make the price of microalgae biomass remain high and become a bottleneck restricting the industrial production of microalgae. Therefore, screening microalgae with high oil content from various microalgae and screening culture methods to improve the oil content and quality of microalgae are one of the hot spots in microalgae research at present. [0003] Yellow algae Tribonema sp. is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/41C12R1/01
CPCC12N1/12C12N1/20
Inventor 霍书豪刘芬陈静邹彬张存胜朱菲菲
Owner JIANGSU UNIV
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