A method for increasing oil content and fatty acid content in Xanthophyllum algae
A technology of oil content and yellow silk algae, applied in the field of biotechnology or food, can solve the problems affecting the growth and accumulation of algae biomass, and achieve the effects of reducing composition content, simplifying steps, and simple operation
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Embodiment 1
[0021] Pour the prepared fresh BG11 medium into the light column reactor, and insert the logarithmic phase of Xanthophyllum, the inoculum amount is 10%. Then Rhizobium rosette ( Rhizobium rosetiformans ) cultured for 1d-10d, washed with sterile water and inserted into Rhizobium rosette ( Rhizobium rosetiformans , R group), the inoculum volume was 1%; the blank group (CK group) was not inoculated with bacteria. Place in a climate incubator, set the temperature at 28°C, light for 24 hours, light intensity 40%, and directly pass into the environment mixed with 5% CO 2 air, the ventilation volume is about 0.5vvm. Such as figure 1 As shown in Table 1, after culturing for one week, in the experimental group in which the bacteria were inserted, the oil content was 53% of the dry weight of the yellow silk algae, and the oil content was higher than that of the CK group (43%). The oil accumulation of algae has a promoting effect, and the amount of oil accumulation has increased b...
Embodiment 2
[0023] Pour the prepared fresh BG11 culture medium into the light column reactor, and insert the Zytophthora algae, the inoculum amount is 10%. Hydrogenophage xanthus ( Hydrogenophaga intermedia ) cultured for 1d-10d, washed with sterile water and inserted into the yellow hydrogen phagocytosis ( Hydrogenophaga intermedia ) (Group H), the inoculation volume was 1%. Blank group (CK group), no bacteria were inserted. Place in a climate incubator, set the temperature at 28°C, light for 24 hours, light intensity 40%, and directly pass into the environment mixed with 5% CO 2 air, the ventilation volume is about 0.5vvm. Such as figure 1 As shown in Table 1, after culturing for one week, in the experimental group inoculated with bacteria, the oil content was 45.67% of the dry weight of Xanthophyllum, and the oil content was higher than that of the CK group (43%). Oil accumulation has a promoting effect. The accumulation of saturated fatty acids (Saturated fatty acids, SFA) in...
Embodiment 3
[0025] Pour the prepared fresh BG11 medium into the light column reactor, and insert the logarithmic phase of Xanthophyllum, the inoculum amount is 10%. Then the sphingosine bacteria ( Sphingopyxis ummariensis ) cultured for 1d-10d, washed with sterile water and inserted into the sphingosine box bacteria ( Sphingopyxis ummariensis , S group), the inoculation volume was 1%. The blank group (CK group) was not inoculated with bacteria. Place in a climate incubator, set the temperature at 28°C, light for 24 hours, light intensity 40%, and directly pass into the environment mixed with 5% CO 2 air, the ventilation volume is about 0.5vvm. Such as figure 1 As shown in Table 1, after one week of culture, the oil content was 48.5% of the dry weight of Xanthophylla, and the oil content was higher than that of the CK group (43%). The effect of oil accumulation increased by 12.79% was more significant. The accumulation of saturated fatty acids (Saturated fatty acids, SFA) in Xanth...
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