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A kind of method for inducing callus formation of Ashwagandha

A multi-flowered tree and callus technology, applied in gardening methods, botanical equipment and methods, plant regeneration, etc., can solve the problem of low reproduction coefficient

Active Publication Date: 2019-09-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] A. multiflora is a newly introduced species, so there are few domestic studies on tissue culture of A. multiflora. However, a few scholars have conducted some tissue culture research on other plants of the genus A. multiflora, but the reproduction coefficient is relatively low.

Method used

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  • A kind of method for inducing callus formation of Ashwagandha
  • A kind of method for inducing callus formation of Ashwagandha
  • A kind of method for inducing callus formation of Ashwagandha

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Experimental program
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Embodiment 1

[0022] 1. Collection and cleaning of explants

[0023] Collect the tender stems of the branches and stems of the multi-flowered wood, cut the stems about 1-3cm long, put them in a white porcelain tank, and wash them with water with a small amount of washing powder for 2 minutes, wash the foam and rinse under tap water 2h. The explants removed from the surface dust and soil were placed on an ultra-clean workbench for disinfection.

[0024] 2. Disinfection treatment

[0025] Firstly treat with 75% alcohol for 30-50s, then wash once with sterile water, then disinfect with 0.1% mercuric chloride for 3-6 minutes, then rinse with sterile water for more than 3 times, and use sterile water to clean the remaining water. Bacteria paper absorption.

[0026] 3. Carry out inoculation work

[0027] Place the sterilized stems on sterile paper and inoculate them on the culture medium with tweezers. The culture medium is respectively WPM, MS, 1 / 2MS, B5, N6, and different concentrations of ...

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Abstract

The invention discloses a method for inducing calluses of flowering dogwood. The method comprises the following steps: by taking a stem of flowering dogwood as an explant, sterilizing, inoculating with a culture medium, and performing lighting culture, wherein the formula of the culture medium comprises a basic culture medium, 1-2mg / L 6-BA, 0.1-0.3mg / L NAA, 30-40g / L of cane sugar and 6-9g / L agar. The invention explores an active and effective novel floribunda flowering dogwood stem tissue culture method, culture mediums, culture conditions and sterilization methods are optimized, and a great amount of calluses can be obtained through induction within a short time.

Description

technical field [0001] The present invention relates to tissue culture, in particular to a method for inducing the formation of callus of A. multiflora. Background technique [0002] Multiflora, Cornaceae, and the genus, also known as the big-flowered Sizhaohua, is native to eastern North America. The multi-flowered tree has different scenery in four seasons, with petal-shaped bracts in full bloom in spring and colorful leaves in summer and autumn. , with orange-red berries after flowering, it is a high-grade landscape plant and oil plant with a broad market prospect, and our country is still in the stage of introduction. The multi-flowered tree is generally propagated by seed sowing and cuttings. This patent carries out tissue culture and propagation on the multiflora tree, and promotes the formation of callus by cultivating the organ tissue of the multiflower tree, thereby cultivating more seedlings of the multiflower tree, which is the multiflora tissue important way to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘卫周余华史卓林顾敏敏胡修武侯波
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY