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A strain of Rhodopseudomonas palustris hew-gj106 and its application

A HEW-GJ106, swamp rhodopseudomonas technology, applied in the application, bacteria, microorganisms and other directions, to achieve the effect of promoting the absorption and utilization of nutrients, strong fermentation, and promoting animal growth

Active Publication Date: 2020-11-10
BEIJING HESWOF BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the object of the present invention provides a faster propagation speed, which can improve animal production performance and can improve water quality. Rhodopseudomonas palustris (Rhodopseudomonas palustris) HEW-GJ106 with stronger probiotics, to overcome existing swamps Defects in Rhodopseudomonas

Method used

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  • A strain of Rhodopseudomonas palustris hew-gj106 and its application
  • A strain of Rhodopseudomonas palustris hew-gj106 and its application
  • A strain of Rhodopseudomonas palustris hew-gj106 and its application

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Embodiment 1

[0032] Example 1 Isolation, screening, identification and preservation of Rhodopseudomonas palustris HEW-GJ106

[0033] 1. Isolation and purification of Rhodopseudomonas palustris:

[0034] Under aseptic conditions, 1g of aquaculture bottom mud was collected from an aquaculture farm in Hebei, placed in a centrifuge tube filled with 4.5mL of sterilized 0.85wt% physiological saline, oscillated at 200r / min for 30min and mixed, and placed in a photosynthetic bacteria medium Stretch on the flat plate, then pour a layer of photosynthetic bacteria culture medium on it, and adopt anaerobic culture method (5% CO2) after cooling to room temperature. 2 (v / v)), after cultivating the coated plate for 96 hours under light at 30°C (illumination intensity 3500LX), use an inoculation loop to pick brown-red colonies on the photosynthetic bacteria medium plate for streaking, separation and purification, and pick after culture The colony with better separation effect (single colony with vigorous...

Embodiment 2

[0053] Embodiment 2 Preparation of Rhodopseudomonas palustris HEW-GJ106 fermentation broth

[0054] After thawing Rhodopseudomonas palustris HEW-GJ106 frozen at -20°C, streaked on the photosynthetic bacteria medium under sterile conditions, cultured for 4 days at 30°C, with a light intensity of 3500LX, to obtain Rhodopseudomonas palustris Pick a single colony of bacteria HEW-GJ106, pick a single colony in the seed medium, and cultivate it for 4 days at 30°C with a light intensity of 3500LX to obtain a seed solution of Rhodopseudomonas palustris HEW-GJ106, with an activity of 10 9 CFU / mL.

[0055] The photosynthetic bacteria medium is composed of the following components by weight percentage: 0.5% sodium bicarbonate; 0.05% magnesium sulfate heptahydrate; 0.1% ammonium sulfate; 0.05% dipotassium hydrogen phosphate; 0.02% sodium chloride; 0.5%; yeast powder 0.15%; agar 1.5%; the balance is water, pH7.0±0.2.

[0056] The seed medium is composed of the following components by wei...

Embodiment 3

[0063] Example 3 Probiotic Verification of Rhodopseudomonas palustris HEW-GJ106

[0064] On the aseptic operating table, the concentration is 10 9 CFU / mLd of pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Salmonella, Aeromonas hydrophila) bacterial suspension 50mL mixed with equal volume of NA medium (after sterilization) cooled to 45 ℃, prepared into 4mm For the left and right pathogenic bacteria NA plates, place the sterilized Oxford cup on the medium, press gently to make it contact with the medium without gaps, and after 10 minutes, add 200 μL of the preserved example to each small tube dropwise. 2. Keep the prepared fermentation broth from overflowing, incubate at 30°C for 72-96 hours, and then measure the diameter of the inhibition zone. Each experiment was repeated three times, and the average value was taken, and the results are shown in Table 2.

[0065] Wherein, the NA plate of pathogenic bacteria is obtained by mixing the NA medium sterilized at 121...

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Abstract

The invention provides a novel separated rhodopseudomonas palustris strain HEW-GJ106. The strain has a remarkable probiotic property, can remarkably inhibit growth and reproduction of Escherichia coli, Staphylococcus aureus, Salmonella sp., Aeromonashydrophila and other pathogenic bacteria, and has a good antibacterial property.

Description

technical field [0001] The invention relates to photosynthetic bacteria, in particular to a strain of Rhodopseudomonas palustris (Rhodopseudomonas palustris) HEW-GJ106 and application thereof. Background technique [0002] Adding antibiotics to feed can resist disease, promote animal growth, and accelerate the development of intensive animal husbandry. However, with the continuous strengthening of people's concept of green, safety and environmental protection and the continuous emphasis on health, antibiotic additives have brought huge economic benefits to people, and their negative effects have gradually become prominent. The most typical feature is resistance caused by antibiotics. Drug resistance, endogenous infection, superinfection and drug residues pose serious threats to the breeding industry, feed industry and animals, and then endanger human health through the food chain. Therefore, the voice of restricting or even prohibiting the use of antibiotics in the feed ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/01
Inventor 李雪平李成印遇龙
Owner BEIJING HESWOF BIOTECH CO LTD
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