The present invention discloses a sterilization insecticide palm meal fish feed and a preparation method thereof, and the feed comprises the following component in parts by weight: 180-210 parts of palm meal, 35-38 parts of tea seed cake powder, 15-17 parts of chicken liver powder, 23-25 parts of pepper meal, 3-4 parts of edible salt, 5-6 parts of Chinese pear vinegar, 21-25 parts of euphorbia thymifolia, 16-18 parts of Parthenocissus tricuspidata, 12-15 parts of Herba Pteridis multifidae, 24-26 parts of sweet potato powder, 4-6 parts of linseed powder, 1-2 parts of bunge pricklyash leaves, 32-36 parts of pine needles, 17-19 parts of Pennisetum hydridum, 14-16 parts of Artemisia selengensis, 1-2 parts of fish oil, 0.1-0.2 part of EM fermentative bacteria, 5-7 parts of pumpkin protein powder, 2-3 parts of hairyvein agrimony, 1-2 parts of poria, 1-2 parts of radix angelicae pubescentis, 3-4 parts of chitosan and 7-9 parts of whey powder. The sterilization insecticide palm meal fish feed can enhance the immune function of fishes, enhances the resistance of the fishes to bacteria, fungi and protozoa, reduces fish saprolegniasis, white skin disease, red skin disease, gill rot, lepidorthosis and other diseases, and improves fish breeding survival rate and benefits.