Extraction process for Bletilla striata polysaccharide gum

Abstract

The invention belongs to the technical field of traditional Chinese medicinal material extraction, and particularly relates to an extraction process for Bletilla striata polysaccharide gum. The extraction process comprises the following concrete steps: (1) weighing Bletilla striata, carrying out crushing and sieving, and adding water to prepare Bletilla striata slurry; (2) fermenting the Bletillastriata slurry with a complex microbial inoculant, and carrying out primary heating reflux extraction to obtain an extract; (3) filtering the extract, taking filter residues, adding water accounting for 4-5 times the weight of the filter residues into the filter residues, performing secondary heating reflux extraction, and carrying out filtering while the filter residues are still hot so as to obtain a first filtrate and second filter residues; (4) adding water accounting for 2.5-3.5 times the weight of the second filter residues into the second filter residues, performing ultrasonic heating reflux extraction, and carrying out filtering while the second filter residues are still hot to obtain a second filtrate; (5) combining the first filtrate and the second filtrate, carrying out concentrating, adding ethanol, performing standing, and then collecting a precipitate; and (6) washing the precipitate with absolute ethyl alcohol several times, and then drying the precipitate to obtain theBletilla striata polysaccharide gum. Compared with a traditional Bletilla striata extraction method, the method of the invention has the effect of increasing the extraction rate of polysaccharides.

Description

technical field [0001] The invention belongs to the technical field of extraction of traditional Chinese medicinal materials, and in particular relates to an extraction process of bletilla striata polysaccharide gum. Background technique [0002] Bletilla striata is a plant of the genus Bletilla striata in the family Orchidaceae, also known as Xiaobaiji, Lianjicao, Xuerumo, etc. It has been used as traditional Chinese medicine for thousands of years. Bletilla striata bulbs contain a large amount of aqueous polysaccharides, and its chemical composition is mainly glucomannan, which is the main functional component of Bletilla striata gum with extremely small molecular weight. Bletilla striata polysaccharide gum is an excellent natural thickener, a medical raw material with high safety, a pharmaceutical excipient with excellent performance and a biomedical material with considerable development prospects. In addition, Bletilla striata polysaccharide gum can also be used in dai...

AI Technical Summary

Problems solved by technology

However, at present, there are relatively few published technologies about the extraction of Bletilla striata polysaccharide gum. For example, patent application CN201910217453.2 discloses a Bletilla striata polysaccharide extract and its preparation method. The steps of the disclosed Bletilla striata polysaccharide extract preparation method include: (1) Preparation of Bletilla striata powder; (2) Preparation of Bletilla striata water extract; (3) Ethanol precipitation, washing and drying to obtain Bletilla striata crude polysaccharide; (4) Bletilla striata crude polysaccharide was dissolved by adding distilled water, centrifuged, microfiltration, ultrafiltration, concentration, Alcohol precipitation and drying to obtain Bletilla striata polysaccharide extract

Although the extracted polysaccharide gum obtained by the extraction has high purity, the extraction rate is relatively low, and the highest extraction rate is only 35.88%; another patent application CN201710204450.6 discloses a method for extracting bletilla striata polysaccharides, including the following steps: ( 1) Bletilla striata gel preparation; (2) decolorization; (3) protein removal; (4) purification, although the polysaccharide gum extracted by this method can reduce the damage to the polysaccharide, but in the whole process, the fibers that decompose the cell wall are used Sulfase and pectinase are the general term for a class of enzymes that decompose cellulose and pectin respectively. Each contains several types according to their functions and mechanisms of action. The reasonable combination of different types can produce different effects. This article Although the cellulase and pectinase in the application have the effect of decomposing the cell wall, compared with the extraction method without adding the cell wall decomposing enzyme, the extraction rate of the polysaccharide gum can be improved to a certain extent, but the extraction rate is only 35.12%. It shows that the decomposition effect still needs to be improved