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A kind of method for inducing callus originating from the buds of A. multiflora

A multi-flowered ash tree and callus technology, which can be used in horticultural methods, botanical equipment and methods, plant regeneration, etc., and can solve the problems of low reproduction coefficient and the like

Active Publication Date: 2019-08-16
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the newly introduced species of A. multiflora, a few scholars in China have conducted research on other plants of the genus A. genus, but the reproduction coefficient is relatively low.

Method used

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  • A kind of method for inducing callus originating from the buds of A. multiflora
  • A kind of method for inducing callus originating from the buds of A. multiflora
  • A kind of method for inducing callus originating from the buds of A. multiflora

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Experimental program
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Effect test

Embodiment 1

[0020] 1. Arrangement of explants

[0021] Collect the 1-3cm young shoots of the multi-flower tree that were born in the same year, put them into a white porcelain jar, rinse them with washing powder for 2 minutes, and then rinse them under running water for 2 hours. The explants removed from the surface dust and soil were placed on an ultra-clean workbench for disinfection.

[0022] 2. Disinfection treatment

[0023] First treat with 75% alcohol for 20-40s, then rinse once with sterile water, then treat with 0.1% mercury chloride for 3-6 minutes, then rinse with sterile water for 3 times, absorb the residual water with sterile paper .

[0024] 3. Vaccination

[0025] Place the sterilized buds on sterile paper, inoculate them on the medium with sterilized tweezers and scissors, screen different medium WPM, MS, 1 / 2MS, B5, N6, and choose different 6-BA and NAA Concentrations: 6-BA concentrations were 0, 0.5, 1, 2mg / L, and NAA concentrations were 0, 0.02, 0.2, 2mg / L, to study...

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Abstract

The invention discloses an inducing method for a callus tissue derived from flowering dogwood buds. The method comprises the following steps: by taking the flowering dogwood buds as explants, disinfecting and then inoculating onto a culture medium for light culture, wherein the formula of the culture medium is as follows: basal culture medium + 0.2-0.5mg / L 6-BA + 1-2mg / L NAA + 30-40g / L saccharose + 6-9g / L agar. The invention researches a positive and effective new method for the tissue culture of flowering dogwood stems; the culture medium, the culture condition and the disinfecting method are optimized; and a large quantity of callus tissues can be induced and acquired within a short period of time.

Description

technical field [0001] The invention relates to tissue culture, in particular to a method for inducing callus originating from the buds of A. multiflora. Background technique [0002] Multiflora, also known as Dahua Sizhaohua, is native to eastern North America. Multiflower has different scenery in four seasons. In spring, petal-shaped bracts are in full bloom. In summer and autumn, there are colorful leaves and orange-red berries after flowers. , is a high-grade landscape plant and oil plant, the market prospect is broad, and our country is still in the stage of introduction. The multi-flowered tree is generally propagated by seed sowing and cuttings. The formation of callus is promoted by culturing the organ tissues of P. multiflora, thereby cultivating more seedlings of P. multiflora. This is an important way for the propagation of P. multiflora. [0003] Due to the newly introduced species of A. multiflora, a few scholars in China have conducted tissue culture research...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 胡修武周余华史卓林顾敏敏刘卫侯波
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY