A method for assisting the detection of growth traits of cattle with mylk4 gene and its special kit

Abstract

The invention discloses a method for detecting growth character of cow through MYLK4 gene, and a special kit thereof. The method comprises the following steps: by taking cattle genome DNA as a template and a primer pair P as a primer, performing PCR amplification to obtain cattle MYLK4 gene; performing restriction endonucleate HhaI digestion to PCR product, and conducting agarose gel electrophoresis; and according to the electrophoresis result, identifying single nucleotide polymorphism of 61595th cattle MYLK4 gene. The single nucleotide polymorphism of the cattle MYLK4 gene can be detected by using the method and can be used for aided selection and molecular breeding of cattle and quicken the breeding speed of certified seed cattle as a molecular genetic marker closely related to cattle growth character.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to the screening and detection of yellow cattle gene single nucleotide polymorphism (SNP) as a molecular genetic marker, in particular to a method and application for detecting the yellow cattle MYLK4 gene single nucleotide polymorphism. Background technique [0002] The so-called molecular genetic marker is the marker of genetic polymorphism at the DNA level, which can directly reflect the genetic variation on the DNA sequence. Because its source is the difference in DNA sequence, it is also called molecular marker, which has the characteristics of high genetic diversity, strong stability and little environmental influence in biological populations. Molecular marker-assisted selection breeding is a main research content of modern animal molecular breeding. It directly selects the genotype of traits at the DNA level, so the accuracy of its selection is greatly improved, and it overco...

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Problems solved by technology

At present, the research on MYLK4 gene polymorphism at home and abroad is mainly focused on its association with cardiovascular diseases, tumors, etc., but the research on the single nucleotide polymorphism of domestic animal MYLK4 gene has not been reported yet.

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