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A kind of complement c3 detection kit and detection method

A detection kit and kit technology, which is applied in the field of medical immunology in vitro diagnosis, can solve the problems of inaccurate detection results of reagents, failure to meet the requirements of use, and large coefficient of variation, etc., and achieve good stability of reagents, wide versatility, detection The results are accurate

Active Publication Date: 2019-02-12
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Antibodies are prone to flocculent or flaky precipitation during storage, resulting in poor antibody performance, poor repeatability, and large coefficient of variation (CV), directly resulting in inaccurate reagent test results, and additional filtration steps, complicated operation, And filtration may remove antibodies, affect the detection effect, cause certain impact on patients, and cannot meet the requirements for use

Method used

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  • A kind of complement c3 detection kit and detection method
  • A kind of complement c3 detection kit and detection method
  • A kind of complement c3 detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Complement C3 detection kit

[0042] Reagent R1:

[0043] Phosphate buffer

20mmol / L

Sodium chloride

1g / L

Sodium azide

0.5g / L

polyethylene glycol 6000

1g / L

Ethylphenyl polyethylene glycol

1g / L

[0044] Reagent R2:

[0045] TRIS buffer

20mmol / L

Sodium chloride

1g / L

Sodium azide

0.5g / L

Goat anti-human complement C3 antibody

10mg / L

Ethylphenyl polyethylene glycol

1g / L

magnesium sulfate

0.01g / L

calcium acetate

0.01g / L

[0046] Calibrator:

[0047]Complement C3 antigen standard diluent (20mmol / L phosphate buffer, 2g / L sodium chloride, 0.2g / L sodium azide, 0.3g / L dextran, 1g / L trehalose, 2g / L sucrose , 2g / L bovine serum albumin) was dissolved, tested with a commercially available control reagent and adjusted to 120mg / L, and stored in -20°C in aliquots. Take it out before use, and dilute it with standard diluent to obtain...

Embodiment 2

[0048] Example 2 Complement C3 detection kit

[0049] Reagent R1:

[0050] TRIS buffer

60mmol / L

potassium chloride

15g / L

phenol

0.6g / L

polyethylene glycol 6000

30g / L

Ethylphenyl polyethylene glycol

50g / L

[0051] Reagent R2:

[0052] Phosphate buffer

40mmol / L

potassium chloride

15g / L

phenol

0.6g / L

Goat anti-human complement C3 antibody

50mg / L

Ethylphenyl polyethylene glycol

50g / L

magnesium sulfate

16.5g / L

calcium acetate

22g / L

[0053] Calibrator:

[0054] Complement C3 antigen standard diluent (50mmol / L glycine buffer, 10g / L sodium chloride, 0.5g / L sodium azide, 20g / L dextran, 25g / L trehalose, 30g / L sucrose, 20g / L bovine serum albumin) was dissolved, tested with a commercially available control reagent and adjusted to 100mg / L, and stored in -20°C in aliquots. Take it out before use, and dilute it into different concentrations o...

Embodiment 3

[0055] Example 3 Complement C3 detection kit

[0056] Reagent R1:

[0057] acetate buffer

100mmol / L

potassium chloride

20g / L

Sodium azide

0.8g / L

polyethylene glycol 6000

40g / L

Ethylphenyl polyethylene glycol

5g / L

[0058] Reagent R2:

[0059]

[0060]

[0061] Calibrator:

[0062] Complement C3 antigen standard diluent (70mmol / L TRIS buffer, 25g / L sodium chloride, 1g / L sodium azide, 55g / L dextran, 50g / L trehalose, 60g / L sucrose, 75g / L L bovine serum albumin) was dissolved, detected and adjusted to 200 mg / L with a commercially available control reagent, and stored at -20°C in aliquots. Take it out before use, and dilute it into different concentrations of complement C3 standard with standard diluent (complement C3 antigen concentration: 2mg / L, 10mg / L, 40mg / L, 60mg / L, 80mg / L). Then use a 0.65 μm filter membrane to filter and sterilize, and store at 2-8°C.

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Abstract

The invention provides an immunonephelometry detection kit. The immunonephelometry detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains octylphenoxy polyethoxyethanol, the reagent R2 contains octylphenoxy polyethoxyethanol, magnesium salt, calcium acetate and an antibody. The detection kit has the advantages of good performance, good repeatability, and accurate reagent detection result, and can satisfy the usage requirement.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis of medical immunity, in particular to a complement C3 detection kit and detection method. Background technique [0002] Turbidimetry has been widely used in clinical testing. Now the most common application is immunoturbidimetry. [0003] Most of the early immunoassay techniques analyzed the presence and content of specific proteins in the sample to be tested by observing the formation of precipitates, the occurrence of agglutination and hemolysis, and measuring the light scattering caused by aggregates, such as immunodiffusion, Immunoelectrophoresis, direct and brief hemagglutination, passive hemagglutination, complement fixation test, etc. These detection methods are low in cost, easy to judge the results, easy to master technically, and can be widely used to detect various types of clinical samples. However, the above method tends to be eliminated due to its cumbersome operation, time-consum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53
CPCG01N33/53
Inventor 耿英利罗湘宇甘萍萍黎明龙腾镶
Owner SICHUAN MACCURA BIOTECH CO LTD
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