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A kind of prealbumin detection kit and detection method

A technology for detecting kits and prealbumin, which is applied in the field of medical immunology in vitro diagnosis, can solve the problems of inaccurate detection results of the reagents, cannot meet the requirements of use, and affect the detection results, and achieves good stability of the reagents, wide versatility, detection Accurate results

Active Publication Date: 2018-10-12
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Antibodies are prone to flocculent or flaky precipitation during storage, resulting in poor antibody performance, poor repeatability, and large coefficient of variation (CV), directly resulting in inaccurate reagent test results, and additional filtration steps, complicated operation, And filtration may remove antibodies, affect the detection effect, cause certain impact on patients, and cannot meet the requirements for use

Method used

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  • A kind of prealbumin detection kit and detection method
  • A kind of prealbumin detection kit and detection method
  • A kind of prealbumin detection kit and detection method

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0040] In order to enable those skilled in the art to better understand the technical solutions in the application, the present invention will be further described below in conjunction with the embodiments. Obviously, the described embodiments are only some of the embodiments of the application, not all of them. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application. Example 1 Prealbumin Detection Kit

[0041] Reagent R1:

[0042] Phosphate buffer

20mmol / L

Sodium chloride

1g / L

Sodium azide

0.5g / L

polyethylene glycol 6000

1g / L

Ethylphenyl polyethylene glycol

1g / L

[0043] Reagent R2:

[0044] TRIS buffer

20mmol / L

Sodium chloride

1g / L

Sodium azide

0.5g / L

Goat anti-human prealbumin antibody

10mg / L

Ethylphenyl polyethyle...

Embodiment 2

[0047] Example 2 Prealbumin Detection Kit

[0048] Reagent R1:

[0049]

[0050]

[0051] Reagent R2:

[0052] Phosphate buffer

50mmol / L

potassium chloride

15g / L

phenol

0.6g / L

Goat anti-human prealbumin antibody

30mg / L

Ethylphenyl polyethylene glycol

50g / L

magnesium sulfate

16.5g / L

calcium acetate

21g / L

[0053] Calibrator:

[0054] Standard diluent solution for prealbumin antigen (50mmol / L glycine buffer, 10g / L sodium chloride, 0.5g / L sodium azide, 20g / L dextran, 25g / L trehalose, 30g / L sucrose, 20g / L bovine serum albumin) was dissolved, tested with a commercially available control reagent and adjusted to 100mg / L, subpackaged and stored at -20°C. Take it out before use, and dilute it with standard diluent into different concentrations of prealbumin standard (prealbumin antigen concentration: 0mg / L, 10mg / L, 30mg / L, 50mg / L, 70mg / L). Then use a 0.65 μm filter membrane to filter and steri...

Embodiment 3

[0055] Example 3 Prealbumin Detection Kit

[0056] Reagent R1:

[0057] acetate buffer

100mmol / L

potassium chloride

20g / L

Sodium azide

0.8g / L

polyethylene glycol 6000

40g / L

Ethylphenyl polyethylene glycol

5g / L

[0058] Reagent R2:

[0059] acetate buffer

60mmol / L

potassium chloride

20g / L

Sodium azide

0.8g / L

Goat anti-human prealbumin antibody

300mg / L

Ethylphenyl polyethylene glycol

4g / L

magnesium sulfate

0.05g / L

calcium acetate

0.05g / L

[0060] Calibrator:

[0061] Standard diluent for prealbumin antigen (70mmol / L TRIS buffer, 25g / L sodium chloride, 1g / L sodium azide, 55g / L dextran, 50g / L trehalose, 60g / L sucrose, 75g / L bovine serum albumin) was dissolved, tested with a commercially available control reagent and adjusted to 200 mg / L, and stored in -20°C. Take it out before use, and dilute it with standard diluent into different ...

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Abstract

The invention provides an immunonephelometry kit, which contains a reagent R1 and a reagent R2, wherein the reagent R1 contains octylphenoxy polyethoxyethanol, the reagent R2 contains octylphenoxy polyethoxyethanol, magnesium salt, calcium acetate and an antibody. The detection kit has the advantages of good performance, good repeatability, and accurate reagent detection result, and can satisfy the usage requirement.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis of medical immunity, in particular to a prealbumin detection kit and a detection method. Background technique [0002] Turbidimetry has been widely used in clinical testing. Now the most common application is immunoturbidimetry. [0003] Most of the early immunoassay techniques analyzed the presence and content of specific proteins in the sample to be tested by observing the formation of precipitates, the occurrence of agglutination and hemolysis, and measuring the light scattering caused by aggregates, such as immunodiffusion, Immunoelectrophoresis, direct and brief hemagglutination, passive hemagglutination, complement fixation test, etc. These detection methods are low in cost, easy to judge the results, easy to master technically, and can be widely used to detect various types of clinical samples. However, the above method tends to be eliminated due to its cumbersome operation, time-consumi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 耿英利罗湘宇甘萍萍黎明龙腾镶
Owner SICHUAN MACCURA BIOTECH CO LTD
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