Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of quickly analyzing illegal addition of sildenafil analogue in yang-reinforcing health care products

A rapid analysis technology for sildenafil, which is applied in the fields of material analysis, material excitation analysis, material analysis by optical means, etc.

Inactive Publication Date: 2017-12-22
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF6 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the substances separated by TLC can only be judged whether there is doping according to the spots and ratio shift values ​​of the substances.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of quickly analyzing illegal addition of sildenafil analogue in yang-reinforcing health care products
  • Method of quickly analyzing illegal addition of sildenafil analogue in yang-reinforcing health care products
  • Method of quickly analyzing illegal addition of sildenafil analogue in yang-reinforcing health care products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] In this embodiment, the software used includes: drug design platform Discovery Studio 3.0; GAUSSIAN09 program package.

[0085] In this embodiment, the equipment used includes: a portable Raman spectrometer (BWS415, B&W Tek Inc., the United States), with an excitation wavelength of 785nm and a resolution of 5cm -1 , spectral coverage 175-3200cm -1 ; WFH-203B three-purpose UV analyzer (Shanghai Jingke Industrial Co., Ltd.); ultrasonic instrument (KUDOS-SK5200HP, China); centrifuge (HERAEUS, FRESCO 17Centrifuge; Thermo Scientific, USA); analytical balance (METTLER AE240, China) ; Standard spectrum processing software OPUS 5.0.

[0086] In this embodiment, the materials used include: thin-layer chromatography separation plate F 254 (10×20cm) (Merck, Darmstadt, Germany); silver nitrate and trisodium citrate (AR, Sinopharm Chemical Reagent Co., Ltd.); other reagents used were analytically pure, purchased from Sinopharm Chemical Reagent Co., Ltd.; chromatographically pure ...

Embodiment 2

[0157] Step A, pretreatment of the sample to be tested: Weigh 1 mg of the Chinese medicine to be tested, dissolve it in 10 ml of methanol, settle to volume for 3 minutes, vortex for 5 minutes, place it in an ultrasonic instrument, extract for 30 minutes, and centrifuge for 10 minutes (5000rpm, 4 ℃), stored in 4 ℃ refrigerator, pending inspection;

[0158] Step B, pipette 1 μL, spot on thin-layer chromatography separation plate F 254 (10×20cm) (Merck, Darmstadt, Germany), the standard sildenafil citrate, hydroxyhomoxidenafil citrate, vardenafil, pseudovardenafil, homeocid Nafil, reddenafil, and tadalafil each take 1 μl, adopt the optimized TLC development conditions, spot the sample at a distance of 1 to 2 cm from the bottom of the thin-layer plate, and use dichloromethane:acetone:ammonia=20:1.5: 0.2 (v / v) unfolded for 25min. Take it out at a distance of 7cm from the top of the thin-layer board, and let it dry, and inspect the thin-layer spots under a 254nm ultraviolet lamp. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of medicine analysis, and particularly provides a method of quickly analyzing illegal addition of a sildenafil analogue in yang-reinforcing health care products and a method of screening quick detection conditions for the sildenafil analogue. The method, through molecular docking, generally considers the intensity of interactions between a ligand (micro-molecules) and an acceptor (bio-macro-molecules), thereby finding compounds which have potential pharmacological activity and may be added as an illegal additive; furthermore, with combination of density functional theory, a theoretical Raman peak position is calculated, and theoretical shared peaks, which are obtained after data handling, are deeply developed; the results are used as an evidence for quickly determining illegally added sildenafil compounds in the health care products in on-site detection. The method is free of an artificially synthesized reference substance, has wide application range and low experiment cost, has simple operation, is quick, is suitable for on-site quick detection, and supplies stronger evidence to identification for the illegally added derivatives in the health care foods.

Description

technical field [0001] The invention relates to the technical field of medical analysis, specifically, a method for quickly analyzing sildenafil analogues illegally added to impotence health care products, and a method for screening rapid detection conditions for sildenafil derivatives, involving the use of The molecular docking method considers the quality of the interaction between the ligand (small molecule) and the receptor (biological macromolecule) as a whole, and finds the best binding mode between them, so as to discover compounds with potential pharmacological activity; further Combining density functional theory to calculate its theoretical Raman peak position, and quickly judge the method of illegally adding nafil compounds in aphrodisiac health care products through the calculated theoretical common peaks. Background technique [0002] Phosphodiesterase type 5 (PDE-5) inhibitors are clinically used for male erectile dysfunction (ED) caused by different etiologies...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N30/90
CPCG01N21/658G01N30/90
Inventor 陆峰吕狄亚曹岩柴逸峰朱臻宇李丹柳燕陈秀娟
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com