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Method for judging tumor-susceptible mouse model

A mouse model and tumor technology, applied in the field of tumor research and treatment, can solve the problems of high requirements on feeding conditions, imprecise data, application limitations, etc., and achieve the effect of simplifying the difficulty of experimental operation, objective and accurate indicators, and reducing costs.

Inactive Publication Date: 2018-01-09
中国医科大学
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The above-mentioned mouse model has the following disadvantages: first, the tumor cells cannot be monitored in the initial stage of growth in the mouse, and the measurement and evaluation of tumor growth has a certain lag; second, the size of the tumor mass needs to be measured in the living mouse , the measurement is subject to interference from subjective factors, and the data is not accurate enough; third, tumor cells may have tissue metastasis, and only the tumor mass on the body surface can be monitored. This model cannot evaluate tumor metastasis, and cannot truly reflect the characteristics of tumor growth and migration Fourth, immunodeficiency mice are in an artificially created abnormal immune function environment, which cannot fully and truly reflect the situation of the physiological microenvironment; fifth, immunodeficiency mice are expensive and require high feeding conditions, and their application is also difficult. therefore restricted

Method used

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  • Method for judging tumor-susceptible mouse model
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  • Method for judging tumor-susceptible mouse model

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1: Applying this method to evaluate macrophages Nrf2 specific knockout mice ( Nrf2 - Whether the (φ)KO) model is tumor susceptible.

[0036] The specific experimental methods, materials, processes, data and conclusions are as follows:

[0037] 1. Main reagents: DMEM medium (Gibco, USA); Fetal bovine serum (FBS) (Biological Industries, Israel); Penicillin (Biological Industries, Israel); Pancreatin (Gibco, USA); Blood sugar test strips.

[0038] 2. Main instruments: ultra-clean bench (Nuair, USA); carbon dioxide incubator (Thermo, USA); inverted microscope (Zeiss, Germany); independent ventilation system for mouse breeding (Suzhou Suhang Technology Equipment Co., Ltd.) ; blood glucose meter (FreeStyle blood glucose detector).

[0039] 3. Cell culture: MIN6 cell line should be normally cultured in DMEM high-glucose medium (15% FBS, 5 μL / L β-mercaptoethanol, 100 units / mL penicillin, 100 μg / mL streptomycin) in 5% CO 2 , 37 ℃ conditions.

[0040] 4. Source an...

Embodiment 2

[0049] Example 2: This method was used to evaluate the effect of lipid membrane-like nuclear transcription factor NFE2L1 on the proliferation of insulinoma cell MIN6.

[0050] The specific experimental methods, materials, processes, data and conclusions are as follows.

[0051] 1. Main reagents: DMEM medium (Gibco, USA); Fetal bovine serum (FBS) (Biological Industries, Israel); Penicillin (Biological Industries, Israel); Pancreatin (Gibco, USA); Blood sugar test strips.

[0052] 2. Main instruments: ultra-clean bench (Nuair, USA); carbon dioxide incubator (Thermo, USA); inverted microscope (Zeiss, Germany); independent ventilation system for mouse breeding (Suzhou Suhang Technology Equipment Co., Ltd.) ; blood glucose meter (FreeStyle blood glucose detector).

[0053] 3. Cell culture: MIN6 cell line should be cultured in DMEM high glucose medium (15% FBS, 5 μL / L β-mercaptoethanol, 100 units / mL penicillin, 100 μg / mL streptomycin) in 5% CO 2 , 37 ℃ conditions. Nfe2l1 Establ...

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Abstract

The invention belongs to the field of tumor research and treatment and particularly relates to a method for judging a tumor-susceptible mouse model. The proliferation situation of insulinoma cell canbe directly reacted through dynamic monitoring of blood glucose in a mouse; when the insulin in the mouse reaches a certain limit, the mouse can die of severe hypoglycemia caused by excessive insulinsecretion, and the reason is caused by the proliferation of the MIN6 cells; as a tumor microenvironment existing in a mouse body promotes the proliferation, mice living under the same conditions are proved to be prone to tumors, which is the tumor-susceptible mouse model.

Description

technical field [0001] The invention belongs to the field of tumor research and treatment, and in particular relates to a method for judging a tumor-susceptible mouse model. Background technique [0002] Tumor (tumour) refers to the neogrowth formed by the proliferation of local histiocytes under the action of various tumorigenic factors, because most of these neogrowths are space-occupying block-like protrusions, also known as neoplasms (neoplasm) . The study of tumor pathogenesis and tumor treatment is mainly divided into two aspects: one is for the tumor cells themselves, and the other is for the environment required for the growth of tumor cells. [0003] At present, in order to study the pathogenesis of tumors and propose new prevention and treatment strategies, many researchers have established various mouse research models, including inoculating human-derived tumor cells into immunodeficient mice, and measuring the size of tumors in mice. Reflecting the characterist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/13A61K49/00
Inventor 富景奇皮静波王惠惠陈承杰包思萌徐子晋
Owner 中国医科大学
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