Gynura formosana Kitam. total flavonoids extract, preparation method thereof, and applications of gynura formosana Kitam. total flavonoids extract in treating acute pharyngitis

A technology of total flavonoids and extracts, which is applied in the field of total flavonoids extract and its preparation in the treatment of acute pharyngitis, can solve the problems of acute pharyngitis without Baifengcai and without Baifengcai extract, and achieve the relief of acute pharyngitis Symptoms, improvement of extraction rate, improvement of pathological changes

Inactive Publication Date: 2018-01-16
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in the prior art, there is no related report that Baifengcai can be used to treat acute pharyngitis, and there is no related report that Baifengcai extract can be used to treat acute pharyngitis

Method used

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  • Gynura formosana Kitam. total flavonoids extract, preparation method thereof, and applications of gynura formosana Kitam. total flavonoids extract in treating acute pharyngitis
  • Gynura formosana Kitam. total flavonoids extract, preparation method thereof, and applications of gynura formosana Kitam. total flavonoids extract in treating acute pharyngitis
  • Gynura formosana Kitam. total flavonoids extract, preparation method thereof, and applications of gynura formosana Kitam. total flavonoids extract in treating acute pharyngitis

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Experimental program
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Embodiment 1

[0042] In this embodiment, the total flavonoids extract of Baifengcai is prepared according to the following method:

[0043] (1) Extraction: take 100 g of basilisk, add 30 times the amount of water by weight to extract, adjust the pH of the extract with dilute hydrochloric acid to be 5, and obtain the reaction solution;

[0044] (2) Enzymolysis: Then add 25g of compound enzyme (the compound enzyme is composed of papain, cellulase and pectinase in a weight ratio of 1:3:2) to the reaction solution for enzymolysis, and the reaction is forced to circulate at 40°C 3 hours, suction filtration, collect filtrate;

[0045](3) Extraction and concentration: add the filtrate to the extraction tank filled with AB-8 macroporous resin, stir at 30°C and 100rpm for 12 hours, filter, and use the weight of AB-8 macroporous resin to take The AB-8 macroporous resin was added 20 times by weight of an ethanol solution with a volume concentration of 75%, then stirred at 30°C and 120rpm for 12 hours...

Embodiment 2

[0063] In this embodiment, the total flavonoids extract of Baifengcai is prepared according to the following method:

[0064] (1) Extraction: get 100 g of Baifengcai, add 20 times the amount of water by weight to extract, adjust the pH value of the extract to 8 with dilute sodium hydroxide solution, and obtain the reaction solution;

[0065] (2) Enzymolysis: Then add 20g of compound enzyme (the compound enzyme is composed of papain, cellulase and pectinase with a weight ratio of 0.5:5:1) to the reaction solution for enzymolysis, and the reaction is forced to circulate at 30°C 4 hours, suction filtration, collect filtrate;

[0066] (3) Extraction and concentration: add the filtrate to the extraction tank filled with DM-130 macroporous resin, stir at 30°C and 80rpm for 24 hours, filter, and use the weight of DM-130 macroporous resin to take after adsorption The DM-130 macroporous resin was added 10 times by weight of ethanol solution with a volume concentration of 95%, then sti...

Embodiment 3

[0071] In this embodiment, the total flavonoids extract of Baifengcai is prepared according to the following method:

[0072] (1) Extraction: take 100 g of basilisk, add 60 times the amount of water by weight to extract, adjust the pH value of the extract to 4 with dilute hydrochloric acid, and obtain the reaction solution;

[0073] (2) Enzymolysis: Then add 32g of compound enzyme (the compound enzyme is composed of papain, cellulase and pectinase with a weight ratio of 1.5:2:3) to the reaction solution for enzymolysis, and the reaction is forced to circulate at 50°C 1 hour, suction filtration, and collect the filtrate;

[0074] (3) Extraction and concentration: Add the filtrate to the extraction tank filled with ZH-01 macroporous resin, stir at 30°C and 150 rpm for 6 hours, filter, and take the weight of ZH-01 macroporous resin to take after adsorption The ZH-01 macroporous resin was added 30 times by weight of ethanol solution with a volume concentration of 70%, then stirre...

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Abstract

The invention belongs to the field of drug or health care product, and more specifically relates to a gynura formosana Kitam. total flavonoids extract, a preparation method thereof, and applications of the gynura formosana Kitam. total flavonoids extract in treating acute pharyngitis. The gynura formosana Kitam. total flavonoids extract comprises, by weight, 80 to 85% of rutin. It is shown by drugeffect experiment results that the gynura formosana Kitam. total flavonoids extract possesses certain curative effect on mice acute pharyngitis models, is capable of relieving symptoms of mouse acutepharyngitis, and improving pathological changes of mouse pharyngeal mucosa, and can be taken as a potential drug used for treating acute pharyngitis. According to the preparation method, after extraction, a composite enzyme is composed of enzymes of specific composition and specific ratio, and is used for enzymatic hydrolysis; and in addition, macroporous resin extraction condensation and macroporous resin separation purifying are adopted. The HPLC purity of rutin in the gynura formosana Kitam. total flavonoids extract is capable of reaching 80 to 85%.

Description

technical field [0001] The invention belongs to the field of medicines or health care products, and in particular relates to an extract of total flavonoids from baifengcai, a preparation method thereof and an application for treating acute pharyngitis. Background technique [0002] Acute pharyngitis belongs to the category of "acute throat arthralgia" in traditional Chinese medicine. Lymphatic follicles and pharyngeal lateral cord are red and swollen, submandibular lymph nodes are enlarged and tender, and the main manifestations are wind-heat symptoms or lung-stomach excess heat syndrome. Acute pharyngitis is a common and frequently-occurring disease in the Department of Otorhinolaryngology. Severe cases of acute pharyngitis can cause many complications and cause great harm. Western medicine mainly uses antibiotics, but there are disadvantages such as large toxic side effects, double infection, and easy drug resistance. . The traditional Chinese medicine treatment of acute...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P11/04A23L33/105
Inventor 陈志亮林锦霞于娟夏裕发殷婷婷杨小平
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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