Building method of angelica dahurica tissue culture system

A method of establishment, the technology of Angelica dahurica, applied in the field of plant tissue culture, can solve the problems of high cost, low germination rate, and difficult germination, and achieve the effect of large market sales, large reproduction coefficient, and high seedling rate

Inactive Publication Date: 2018-01-19
陈金水
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When using seeds for propagation, the seeds have the problems of difficult germination and low germination rate, which greatly reduces the survival rate and seedling rate, and the cost is high and the income is l

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Disinfection of explants: Rinse the collected stems of Angelica dahurica with clear water for 10 minutes and gently brush off the impurities on them with a brush. Disinfect them with 75% ethanol solution for 9 seconds in an ultra-clean workbench, and then wash them with sterile water 3 times, then rinse with 5% Antifoam solution for 4 minutes, rinse 3 times with sterile water, and then use sterile filter paper to absorb the water and set aside.

[0017] (2) Induction culture: cut the stems of Angelica dahurica sterilized in step (1) into 1.4cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate them into the induction culture Bud induction culture was carried out in the medium. After inoculation, they were first cultured in total darkness at 24°C for 2 days, and then placed in light for 9 hours a day with a light intensity of 1900 lx, and cultured at a temperature of 24°C until ad...

Embodiment 2

[0022] (1) Disinfection of explants: the collected stems of Angelica dahurica were rinsed with clean water for 11 minutes, and the impurities on them were gently brushed off with a brush, disinfected with 76% ethanol solution in an ultra-clean workbench for 19 seconds, and then washed with sterile water 4 times, then rinse with 5% Antifoam solution for 7 minutes, rinse with sterile water for 5 times, and then use sterile filter paper to absorb the water before use.

[0023] (2) Induction culture: cut the stems of Angelica dahurica sterilized in step (1) into 1.4cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate them into the induction culture Bud induction culture was carried out in the medium. After inoculation, they were first cultured in total darkness at 27°C for 3 days, and then placed in light for 11 hours a day with a light intensity of 2400 lx, and cultured at a temperature of 27...

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PUM

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Abstract

The invention discloses a building method of an angelica dahurica tissue culture system. Angelica dahurica belongs to perennial tall herbs, and the height of the angelica dahurica is 1 to 2m; roots ofthe angelica dahurica are in a cylindrical or circular cone shapes; the roots have branches and the color of surfaces of the roots is yellowish-brown; stems are hollow and are provided with lengthwise grooves; foundation parts of the stems are thick and big without hairs, and the foundation parts are generally purple; leaves of the angelica dahurica alternate and are pinnately divided, the tipsof the leaves are pointed sharply, the edges of the leaves are provided with irregular sawteeth; the angelica dahurica blossoms in July to August, the angelica dahurica bears fruits in August to September, and the fruits are in oblong or oval shapes. The roots can be applied to medicines, are excavated in summer and autumn, and are dried in the sun for subsequent use. According to the building method of the angelica dahurica tissue culture system disclosed by the invention, steam sections with buds are explants and are subjected to induction, proliferation, rooting, exercising seedling, transplanting and the like so as to successfully obtain angelica dahurica tissue culture regenerated plants, and a systematical angelica dahurica tissue culture plant regeneration system is built. The angelica dahurica is in great demand in the domestic market, and an angelica dahurica asexual propagation system is built, so that reproduction of the angelica dahurica is promoted; by reasonably utilizingresources of the angelica dahurica, the good ecological benefit, the economic benefit and the market benefit can be achieved.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for establishing a tissue culture system of Angelica dahurica. Background technique [0002] Baizhi is also known as: Xing'an Baizhi, Zouma Zhi, Fenxiang, Zefen, Xiang Baizhi. It is a perennial tall herb, 1-2 meters high. Root cylindrical or conical, branched, surface yellowish brown. The stem is hollow, with longitudinal grooves, thick base, glabrous, usually purple. The leaves are alternate, pinnately divided, with sharp tips and irregular serrations on the edges. It blooms from July to August, and the flowers are white, arranged in compound umbels and born on the tops or sides of branches. Results from August to September, the fruit is oblong or oval, nearly spongy, with side ribs and wings. The roots can be used as medicine, excavated in summer and autumn, and dried in the sun for later use. It grows in Shanxi, Henan, Hebei, Hun...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈金水
Owner 陈金水
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