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Amino acid-based antibacterial hydrogel containing nano-silver, and preparation method and use thereof

A hydrogel and amino acid technology, applied in the field of medical materials, can solve the problems of poor biocompatibility, difficult degradation, high toxicity, etc., and achieve biocompatibility, biodegradability, stable and uniform gel, and good biocompatibility Effect

Active Publication Date: 2018-01-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, amino acid-based antibacterial hydrogels can solve the disadvantages of existing antibacterial hydrogels such as poor biocompatibility, difficult degradation, high toxicity, and poor activity, and are expected to play an important role in biomedical dressings, tissue repair, and burn wound care.

Method used

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  • Amino acid-based antibacterial hydrogel containing nano-silver, and preparation method and use thereof
  • Amino acid-based antibacterial hydrogel containing nano-silver, and preparation method and use thereof
  • Amino acid-based antibacterial hydrogel containing nano-silver, and preparation method and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 4 mg of N-fluorenylmethoxycarbonyl-protected histidine (Fmoc-His-OH) was pre-dissolved in Tris buffer solution, ultrasonically assisted to dissolve completely, and the pH value of the solution was adjusted to neutral. Prepare 10mg mL -1 Take 500uL of silver nitrate aqueous solution, add 500uL to the above solution and mix evenly, place it under 20kHz ultrasonic to react for 10min, then let it stand at room temperature for aging reaction for 3d, and finally obtain the hydrogel containing nano-silver in amino acid.

[0055] The transmission electron microscope and atomic force microscope pictures of the obtained hydrogel sample show a good three-dimensional network structure and uniformly distributed nano-silver particles, as shown in the attached figure 1 shown.

Embodiment 2

[0057] Pre-dissolve 5mg of N-fluorenylmethoxycarbonyl-protected proline (Fmoc-L-Pro) in 20uL of ethanol solution to prepare a tris(hydroxymethyl)aminomethane (Tris) solution with a concentration of 5mg mL -1 , take 300uL and add it to the above ethanol solution, mix well. Prepare aspirin ethanol solution, the concentration is 100mg mL -1 , Take 100uL and add to the above solution, mix well. Adjust the pH value to 6.8 and place it at room temperature for later use. Prepare 10mg mL -1 Take 500uL of silver trifluoroacetate (CF3COOAg) solution, add 500uL to the above solution and mix evenly, put it under 40kHz ultrasonic for 5min, then let it stand at room temperature for aging reaction for 7d, and finally get aspirin-loaded proline-containing nano Silver hydrogel.

[0058] The physical pictures of the obtained hydrogel samples are attached figure 2 , placed at a slant, the sample does not flow and is in a gel state.

Embodiment 3

[0060] Pre-dissolve 5 mg of phenyl (Azo-)-protected alanine (phenylalanine, Azo-Ala) in 10 uL ethanol solution to prepare tris(hydroxymethyl)aminomethane (Tris) solution with a concentration of 10 mg mL -1 , take 500uL and add it to the above ethanol solution, mix well. Prepare ethanol solution of ibuprofen, the concentration is 200 mg mL -1 , Take 100uL and add to the above solution, mix well. Adjust the pH value to 6.5 and place it at room temperature for later use. Prepare 5mg mL -1 sulfadiazine silver ammonia solution, take 500uL and add it to the above solution and mix evenly, put it under 20kHz ultrasound for 10min, and then let it stand at room temperature for aging reaction for 7d, and finally get the alanine-containing nano-silver containing ibuprofen. of hydrogel.

[0061] The obtained hydrogel sample has obvious inhibitory effect on Escherichia coli and Staphylococcus aureus, and the results of the inhibition zone test are shown in the attached image 3 .

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Abstract

The invention discloses an amino acid-based antibacterial hydrogel containing nano-silver, and a preparation method and a use thereof. The antibacterial hydrogel containing nano-silver is formed through co-assembling amino acid and silver nitrate. The amino acid hydrogel has the advantages of flexible and adjustable mechanical performances, uniform nano-silver distribution, broad-spectrum continuous antibacterial performance, stable and uniform gel, good biocompatibility, biodegradability, and simplicity in preparation, and is expected to play a great role in the fields of biological nano-materials, aseptic treatment, wound dressings and burn wound care.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to an amino acid-based broad-spectrum antibacterial hydrogel containing nano-silver and its preparation method and application. Background technique [0002] Hydrogel antibacterial materials have important applications in the field of biomedicine, because of their good fluidity and spreadability, good adsorption, and continuous antibacterial properties, and the three-dimensional network structure of the gel has a variety of effective anti-inflammatory and analgesic properties. The ability of the composition is widely used as bio-nanomaterials, wound dressings, etc. [0003] Among them, due to the excellent broad-spectrum antibacterial activity of nano-silver, nano-silver-based hydrogel materials play an important role in the field of biomedicine. However, the existing nano-silver antibacterial hydrogel materials have cumbersome preparation process, poor biocompatibility, difficult ...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K45/06A61K33/38A61K47/18A61P31/04A61P17/02A61L26/00A61K31/192A61K31/365
Inventor 闫学海邢蕊蕊宋静文袁成前李淑坤
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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