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Application of scd1, a marker molecule for detection of drug resistance of glioma temozolomide

A brain glioma, temozolomide technology, applied in the field of biomedicine, can solve the problems of unsatisfactory treatment effect, easy recurrence of patients, poor prognosis, etc., and achieve the effect of high scientific research and production application value

Active Publication Date: 2020-03-17
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional treatment methods for patients with glioma mainly include surgical resection, radiotherapy and chemotherapy. Although these treatment methods can prolong the median survival time of patients to 14.6 months, the treatment effect is not ideal, and patients are prone to relapse, resulting in a poorer prognosis

Method used

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  • Application of scd1, a marker molecule for detection of drug resistance of glioma temozolomide
  • Application of scd1, a marker molecule for detection of drug resistance of glioma temozolomide
  • Application of scd1, a marker molecule for detection of drug resistance of glioma temozolomide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 The IC50 of the glioma temozolomide-resistant cell line is significantly increased

[0028] IC50 (half maximal inhibitory concentration) refers to the concentration at which the measured drug induces 50% tumor cell death. The IC50 value can be used to measure the ability of the drug to induce apoptosis. That is, the stronger the ability to promote apoptosis, the lower the value, indicating that the drug The better the sensitivity, of course, it can also reversely explain the tolerance of a certain cell to the drug. The parental cell line and glioma temozolomide-resistant cell line were treated with different concentrations of temozolomide, and the IC50 value of temozolomide was calculated by using the cell growth curve to confirm the successful construction of the temozolomide-resistant cell line of glioma; Time to further verify the construction of glioma temozolomide-resistant cell lines.

[0029] The experimental method is as follows: brain glioma cells we...

Embodiment 2

[0031] Example 2 High expression of SCD1 in temozolomide-resistant cell lines

[0032] Studies have shown that SCD1 participates in the occurrence and development of tumors by regulating fatty acid metabolism. The expression levels of SCD1 in glioma temozolomide-resistant cell lines were evaluated by qPCR and western blot respectively.

[0033] The experimental method is as follows:

[0034] 2.1 Find the full-length sequence of SCD1 gene mRNA

[0035] Search for SCD1 in the PUBMED Nucleotide database, select the full-length SCD1 mRNA of human origin (Homo sapiens), the sequence number is NCBI Reference Sequence: NM_005063.4, the full-length contains 5473 nucleotides, and the sequence is as follows:

[0036]

[0037]

[0038]

[0039]

[0040]

[0041]

[0042]

[0043]

[0044] 2.2 Design of SCD1 primers

[0045] In the PrimerBank database, select NCBI Gene Symbol, select Homo sapiens for species, and enter SCD1 for For text; or select GenBank Accessio...

Embodiment 3

[0049] Example 3 Overexpression of SCD1 can block the inhibitory effect of temozolomide on parental cell lines

[0050] In order to clarify whether the overexpression of SCD1 affects the reactivity of the parental cell line to temozolomide, the antitumor effect of temozolomide after the overexpression of SCD1 in the parental cell line was detected by drawing a survival curve, and the effect of overexpression of SCD1 in the parental cell line on the efficacy of temozolomide was evaluated. .

[0051] The experimental method is as follows: Take the parental glioma cell line in good condition and in the logarithmic growth phase, overexpress SCD1, and then culture it in a 96-well plate. When it reaches 70-80% confluence, treat it with different high concentrations of temozolomide Then add 20 μL of MTS reagent to the cell line, incubate for 4 hours, measure the absorbance value at a wavelength of 490 nm, draw the cell growth curve, and calculate the IC50 value of temozolomide.

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Abstract

The invention provides application of a brain glioma temozolomide drug resistance detection marker molecule SCD1, and particularly relates to a reagent for detecting the expression quantity of SCD1 inbrain glioma cells and preparing a preparation for predicting drug resistance of brain glioma to temozolomide and application of an inhibitor of SCD1 in preparation of a preparation for promoting drug resistance sensitivity of the brain glioma to the temozolomide. It is first discovered that the expression quantity of SCD1 in the brain glioma cells is related to the drug resistance of the brain glioma to the temozolomide, and it discovers that the inhibitor of SCD1 can promote the drug resistance sensitivity of the brain glioma to the temozolomide. The application has important significance on research of treatment of the brain glioma.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the application of SCD1, a marker molecule for detecting drug resistance of glioma temozolomide. It specifically relates to the reagents for detecting the expression of SCD1 in glioma cells for the preparation of preparations for predicting drug resistance of gliomas to temozolomide, and the use of SCD1 inhibitors in the preparation of sensitive preparations for promoting the resistance of gliomas to temozolomide on the application. Background technique [0002] Gliomas, malignant progressive astrocytomas, are one of the leading causes of cancer-related death in adults and young children worldwide. After initial diagnosis, the median survival time of patients with glioma is about 12-15 months. At present, the traditional treatment methods for patients with glioma mainly include surgical resection, radiotherapy and chemotherapy. Although these treatment methods can prolong the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61P35/00A61P25/00
Inventor 龚志成孙仑泉李学军霍雷徐志杰颜元良熊小明戴爽钱龙曾双双
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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