Method for industrial large-scale production of flavones-enriched Tianshan Tianshan saussurea involucrate culture

A kind of snow lotus culture and large-scale technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of harsh growth conditions, low reproduction rate, and insufficient resources of Tianshan snow lotus, and achieve adaptation to normal temperature Effects of growth conditions, short production cycle, and stable traits

Inactive Publication Date: 2018-02-02
JIANGSU FENGGU BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the harsh growth conditions and low reproductive rate of Tianshan snow lotus, coupled with long-term over-harvesting by people, the resources of Tianshan snow lotus are seriously insufficient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Use Tianshan snow lotus seeds to induce aseptic seedlings after sterilization; the sterilization steps include soaking in 75% ethanol for 30 seconds, washing with sterile water twice, soaking in 0.1% mercury chloride solution for 15 minutes, and washing with sterile water for 3 Inoculate on MS solid medium, light 2000Lux, 12h / d.

[0021] (2) Transfer the sterile seedlings (leaves, roots, stems) to MS-added phytohormone medium to induce callus; the induction medium is 0.5mg / L 6-BA+0.5mg / LNAA+MS+ 200g / sucrose+5.6g / L agar, the culture condition is light 2000Lux, 12h / d.

[0022] (3) Select the callus with the darkest color, close to purple red, and grow well, and subculture it on the screening medium to obtain purple, fast-proliferating, and stable Saussurea culture A; the screening medium is 0.5mg / L 6-BA+0.8mg / LNAA+MS+200g / sucrose+5.6g / agar, the culture condition is light 2000Lux, 12h / d.

[0023] (4) Put Saussurea culture A at 10°C for 20 days, subculture on the scre...

Embodiment 2

[0026] (1) Use Tianshan snow lotus seeds to induce sterile seedlings after sterilization; the sterilization steps include soaking in 75% ethanol for 30 seconds, washing with sterile water three times, soaking in 0.1% mercuric chloride solution for 15 minutes, and washing with sterile water for 5 minutes. Inoculate on MS solid medium, light 3000Lux, 10h / d.

[0027] (2) Transfer the sterile seedlings (leaves, roots, stems) to MS-added phytohormone medium to induce callus; the induction medium is 2.5mg / L6-BA+2.5mg / LNAA+MS+250g / L sucrose+6g / L agar, the culture condition is light 3000Lux, 10h / d.

[0028] (3) Select the callus with the darkest color, close to purple red, and grow well, and subculture it on the screening medium to obtain the purple, fast-proliferating, and stable snow lotus culture A; the screening medium is 2mg / L 6 -BA+2.5mg / LNAA+MS+250g / L sucrose+6g / L agar, the culture condition is light 3000Lux, 10h / d.

[0029] (4) Put Saussurea culture A at 15°C for 15 days, s...

Embodiment 3

[0032] (1) Use Tianshan snow lotus seeds to induce sterile seedlings after sterilization; the sterilization steps include soaking in 75% ethanol for 30 seconds, washing with sterile water twice, soaking in 0.1% mercury chloride solution for 15 minutes, and washing with sterile water for 4 minutes. Inoculate on MS solid medium, light 3000Lux, 11h / d.

[0033] (2) Transfer the sterile seedlings (leaves, roots, stems) to MS medium added with phytohormones to induce callus; the induction medium is 1mg / L 6-BA+2mg / LNAA+MS+250g / L sucrose + 5.8g / L agar, the culture condition is light 2800Lux, 11h / d.

[0034] (3) Select the callus with the darkest color, close to purple red, and grow well, and subculture it on the screening medium to obtain the purple, fast-proliferating, and stable snow lotus culture A; the screening medium is 2mg / L 6 -BA+2mg / L NAA+MS+230g / L sucrose+5.8g / L agar, the culture condition is light 2500Lux, 11h / d.

[0035] (4) Cultivate Saussurea culture A at 13°C for 18 ...

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Abstract

The invention relates to a method for industrial large-scale production of a flavones-enriched Tianshan saussurea involucrate culture and belongs to the technical field of Tianshan saussurea involucrate culture. The method comprises the following steps: inducing Tianshan saussurea involucrate callus, screening Tianshan saussurea involucrate cultures, and performing large-scale production of the Tianshan saussurea involucrate culture. By adopting the method, sterilized Tianshan saussurea involucrate seeds are adopted to culture sterile seedlings, the Tianshan saussurea involucrate callus is induced from the sterile seedlings, culture mediums and culture temperature are changed, the Tianshan saussurea involucrate culture which is stable in proliferation, stable in property and rich in flavones is screened, and the Tianshan saussurea involucrate culture is produced in a large scale in culture modes of solid culture/liquid suspension culture and air-lifting culture. By adopting the method,on the basis of that the content of flavones is ensured, the Tianshan saussurea involucrate culture is stably produced with high yield, and the method has the advantages of being short in productioncycle, unified in product quality and applicable to normal-temperature growth condition.

Description

technical field [0001] The invention relates to a method for industrialized large-scale production of flavonoid-rich Tianshan snow lotus culture, belonging to the technical field of snow lotus cultivation. Background technique [0002] Tianshan Snow Lotus contains flavonoids, alkaloids, polysaccharides and other active ingredients, which have the functions of promoting blood circulation and stimulating menstruation, dispelling cold and dehumidification, stopping bleeding and reducing swelling, and removing toxins from the body. In recent years, the role of Tianshan Snow Lotus as a national medicine and folk medicine in anti-inflammatory and analgesic, anti-early pregnancy, anti-aging and inhibiting the proliferation of cancer cells has attracted much attention. [0003] Tianshan snow lotus grows in rock crevices, stone walls and ice-sulphur gravel beaches near the alpine snow line at an altitude of 2400-4000 meters. Under natural conditions, due to the special growth environ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 高蕴珺
Owner JIANGSU FENGGU BIOLOGICAL CO LTD
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