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Metal fullerol and application of same for preparing drugs for treating leukemia

A technology for fullerol and leukemia, which is applied to metallofullerol and its application field as a drug for the preparation of leukemia, and achieves the effects of reduced toxicity, good selectivity and strong inhibitory effect

Inactive Publication Date: 2018-02-16
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The new nano-medicine of the present invention has obvious growth inhibitory effect on tumor cells in vitro, and works through the mechanism of necrosis or induction of apoptosis respectively. Up to now, it has not been found that these two kinds of fullererols can inhibit the growth of chronic myelogenous leukemia cell line K562 at home and abroad. Study on Anticancer Activity of Cells in Vitro

Method used

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  • Metal fullerol and application of same for preparing drugs for treating leukemia
  • Metal fullerol and application of same for preparing drugs for treating leukemia
  • Metal fullerol and application of same for preparing drugs for treating leukemia

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0031] Gd@C 2m (OH) n preparation of

[0032] First step Gd@C 2m Synthesis

[0033] Mix high-purity (>99.999%) Gd2O3 and high-purity graphite powder (>99.999%) at an atomic ratio of Gd:C=0.5 to 1:50, press into a mold to make a graphite-metal hybrid electrode; or make a graphite-metal hybrid electrode with a diameter of 6 A ~10mm graphite rod is drilled and filled with gadolinium oxide to obtain a graphite-metal hybrid electrode. After sintering at a high temperature of 1000-2000 °C, the metallofullerene Gd@C2m was synthesized using an inert gas arc discharge method. The inert gas is He or Ar, the pressure is 50-600 Torr, and the current is 80-500A.

[0034] The second step Gd@C 82 and Gd@C 60 separation and purification of

[0035] a. Using high temperature reflux plus high temperature and high pressure two-step high-efficiency extraction method to extract and separate carbon nano-like substances. Firstly, the arc-shaped discharge soot is refluxed in toluene at 100-2...

Embodiment 1

[0046] Changes in total cell number

[0047] The K562 cells in the logarithmic growth phase were inoculated in the fresh RPMI1640 medium containing or not containing fullerenol for the test. The test was divided into: negative control group without any drug, C 60 (OH) n Group, 1-100 nm particles [Gd@C 82 (OH) 22 ] x , 1o C , 5%CO 2 The incubator was incubated for 0-6 days, and the total number of cells in each culture well was counted every day. The experiment was repeated 3 times, and the cell growth curve was drawn with the culture time as the abscissa and the mean total number of cells in each group as the ordinate. see results figure 1 , 2 .

[0048] The results found that compared with the control group cells without drug addition, the growth rate of the cells in the drug group was significantly reduced, and the cell doubling time was prolonged. 60 (OH) n It can promote cell proliferation at low concentration and inhibit its growth or induce death at high conce...

Embodiment 2

[0050] Effect of two kinds of fullererols on the proliferation of K562 cells detected by CCK8 method

[0051] The K562 cells in the logarithmic growth phase were inoculated in 96-well culture plates, and different concentrations of fullerol were added to the treatment groups; doxorubicin was used as the positive control group, and blank wells were set. Three replicate wells were set up in each well, and the enzyme-linked immunosorbent assay was used to measure the A 450 value, the cell proliferation inhibition rate was calculated according to the following formula: Proliferation inhibition rate=(A 给药 -A 空白 ) / (A 对照 -A 空白 ) × 100%. see results image 3 , 4 , 5.

[0052] The results showed that: (1) Compared with the negative control group, after 48 hours, fullererol C 60 (OH) n The effect began to appear, and relatively stable, C 60 (OH) n When the concentration is lower than 60 μmol / L, it can promote the proliferation of K562 cells, and when the concentration is high...

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Abstract

The invention relates to a novel application of two kinds of fullerols in biomedicine, in particular to metal fullerol with the general formula of Gd@C82(OH)n and an application of nanoparticles of the metal fullerol in inhibiting growth of human myeloid leukemia cells (K562) or inducing apoptosis of tumor cells, wherein 18 is smaller than or equal to n which is smaller than or equal to 24. The nanoparticles are discovered to have certain inhibition of leukemic cell proliferation activity and are expected to be anti-tumor nano drugs.

Description

technical field [0001] The present invention relates to the new application of two kinds of fullerenols in biomedicine, especially relates to the general formula is C 60 (OH) n and metal fullerenol Gd@C 82 (OH) n The application of nanoparticles in inhibiting the growth of human myeloid leukemia cancer cells (K562) and or inducing tumor cell apoptosis, wherein 18≤n≤24. The nanoparticle was found to have a certain activity of inhibiting the proliferation of leukemia cells, and it can be expected to be used as an antitumor nanomedicine. Background technique [0002] Leukemia, also known as blood cancer, is a kind of clonal malignant disease with abnormal hematopoietic stem cells. Clonal leukemia cells proliferate and accumulate in bone marrow and other hematopoietic tissues due to mechanisms such as uncontrolled proliferation, differentiation disorder, and apoptosis inhibition, and infiltrate other tissues and organs, while normal hematopoiesis is inhibited. Clinically, d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61K33/24A61K41/00A61K51/00A61K9/14A61P35/02
CPCA61K33/44A61K9/14A61K33/24A61K41/0052A61K41/0057A61K51/00A61K2300/00
Inventor 俞梅兰刘星
Owner ZHEJIANG SCI-TECH UNIV
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