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Urine occult blood test paper and production method thereof

A technology for detecting test strips and occult blood, applied in biological testing, material inspection products, etc., can solve the problems of low accuracy and poor stability of detection methods, and achieve the effects of easy visual identification and machine reading, stable performance, and easy operation

Active Publication Date: 2018-03-16
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a urine occult blood detection test paper and its preparation method in order to solve the problems of low accuracy and poor stability of the existing detection methods

Method used

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  • Urine occult blood test paper and production method thereof
  • Urine occult blood test paper and production method thereof
  • Urine occult blood test paper and production method thereof

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preparation example Construction

[0044] The present invention also provides a preparation method of urine occult blood detection test paper, the method comprising:

[0045] Step 1: After immersing the filter paper in liquid A, dry it for the first time at 80-100°C for 20-30 minutes;

[0046] Step 2: After immersing the filter paper dried for the first time in liquid B, carry out the second drying at 40-60°C for 10-20 minutes;

[0047] Step 3: fixing the filter paper after the second drying with the substrate to obtain a urine occult blood detection test paper;

[0048] The A solution includes: buffer, purified water and surfactant A;

[0049] The B liquid includes: ethanol, surfactant B, chromogen, peroxide, and sensitizer;

[0050] The structure of described peroxide is shown in formula I:

[0051]

[0052]In formula I, R1, R2, R3 and R4 are independently selected from -H, -OH, aliphatic or aromatic alcohol substituents, phenol substituents, halogen substituents or C1-C3 alkyl groups.

[0053] Accordi...

Embodiment 1

[0057] Preparation of immersion solution A: phosphate buffer solution (disodium hydrogen phosphate and potassium dihydrogen phosphate buffer solution) (pH=6.0±0.02) 310mL, purified water 670mL, polyvinylpyrrolidone K30 45g.

[0058] Prepare impregnation solution B; ethanol 900mL, polyvinylpyrrolidone K30 25g, 3,3',5,5'-tetramethylbenzidine 2.5g, di-tert-butylperoxycumene 3.0g, 6-methoxy Quinoline 5mL, Tartrazine 260mg, Orange G50mg.

[0059] After the preparation of liquid A and liquid B is completed, first fully immerse the filter paper in liquid A of the impregnating solution, take it out, and dry it at 100°C for 30 minutes, then immerse the dried filter paper in liquid B at 60°C and dry it for 10 minutes, then dry The final filter paper is fixed to the base plate and cut into rectangular paper strips to obtain a urine occult blood test paper.

[0060] The structural formula of described di-tert-butyl peroxide cumene is as follows:

[0061]

Embodiment 2

[0063] Preparation of impregnation solution A: 310 mL of phosphate buffer solution (disodium hydrogen phosphate and potassium dihydrogen phosphate buffer solution, pH=6.0±0.02), 670 mL of purified water, and 45 g of polyvinylpyrrolidone K30.

[0064] Prepare impregnating liquid B; ethanol 900mL, polyvinylpyrrolidone K30 25g, 3,3',5,5'-tetramethylbenzidine 2.5g, di-tert-butyl peroxide cumene 4.8g, 6-methoxy Quinoline 4mL, Tartrazine 260mg, Orange Yellow G 50mg.

[0065] After the preparation of liquid A and liquid B is completed, first fully immerse the filter paper in liquid A of the impregnating solution, take it out, and dry it at 100°C for 30 minutes, then immerse the dried filter paper in liquid B at 60°C and dry it for 10 minutes, then dry The final filter paper is fixed to the base plate and cut into rectangular paper strips to obtain a urine occult blood test paper.

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Abstract

The invention provides a urine occult blood test paper and a production method thereof, and belongs to the field of urine detection. The test paper comprises a substrate and a filter paper fixed on the substrate, and the filter paper is obtained through performing immersion in a solution A and then performing immersion n a solution B; the solution A comprises a buffering solution, purified water and a surfactant A; the solution B comprises ethanol, a surfactant B, a toner, peroxide and a sensitizer; and the structure of the peroxide is represented by formula I. The invention also provides a production method of the urine occult blood test paper. The lowest detection concentration of the occult blood quick test paper produced through the method is 4 Cells / [mu]L, the detection range can reach 4-200 Cells / [mu]L, and the test paper also has the advantages of stable performances, fast test speed and accurate determination result.

Description

technical field [0001] The invention relates to the field of urine detection, in particular to a urine occult blood detection test paper and a preparation method thereof. Background technique [0002] Urinary occult blood, also known as urinary erythrocytes, is the red blood cells that appear in the urine. Urinary erythrocytosis is caused by bleeding in the urinary system (kidneys, bladder, or ureters) where blood enters the urine. This kind of urine sample is also called hematuria. More than 95% of hematuria is caused by diseases of the urinary system itself. Increased urinary red blood cells is a dangerous signal and may be the only clinical manifestation of malignant tumors of the urinary system. [0003] Common causes of urinary erythrocytosis include tuberculosis, tumor, trauma, acute pyelonephritis, urinary stones, acute glomerulitis, and chronic nephritis. Such symptoms require timely medical treatment, so as not to delay the condition and develop into uremia. Ther...

Claims

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Application Information

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IPC IPC(8): G01N33/80G01N33/72
CPCG01N33/72G01N33/80
Inventor 张颖魏学芹赵亚荣崔磊
Owner DIRUI MEDICAL TECH CO LTD
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