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A 3-mcpd detection method and device based on surface molecular imprinting technology

A surface molecular imprinting and detection method technology, which is applied in the direction of measuring devices, analyzing materials, and material analysis through electromagnetic means, can solve the problems of complex oil pretreatment process, high detection limit, and susceptibility to interference, so as to improve detection Sensitivity, interference avoidance, and the effect of improving detection efficiency

Active Publication Date: 2019-12-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention provides a 3-MCPD detection method and device based on surface molecular imprinting technology to overcome the above-mentioned problems or at least partially solve the above-mentioned problems, so as to solve the problem of complex pre-treatment process of grease and detection of 3-MCPD in grease. Technical problems of limited height, low sensitivity, and susceptibility to interference

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] CNTs / SiO prepared by sol-gel method 2 Hybrid material: Add 230 mg of carbon nanotubes into 50 mL of ethanol, and disperse them by ultrasonication for 30 min. Under stirring conditions, 10 mL of benzyl alcohol and 25% ammonia water were sequentially added. 5.0 mL of tetraethoxysilane and 5.0 mL of ethanol were added dropwise to the obtained carbon nanotube suspension. After stirring for 12 hours, the suction filtrate was washed with ethanol to obtain CNTs / SiO 2 Material.

[0031] 1) CNTs / SiO2 2 -Synthesis of MIPs: take 80mg of the above CNTs / SiO 2 Add 0.5mLAPTES and 48.0mL toluene, reflux at 80°C for 20h. Afterwards, 50 mL of absolute ethanol containing 1 mmol / L 3-MCPD was used for centrifugation to obtain 80.0 mg of aminosilane-modified CNTs / SiO 2 . Then use this as the molecular imprinting carrier, 3-MCPD as the template molecule, methacrylic acid (MAA) as the monomer, add 950 μL ethylene glycol dimethacrylate (EGDMA) cross-linking agent, 20.0 mg azobisisobutyro...

Embodiment 2

[0035] The same method as in Example 1 was used, except that the organic phase medium system used 72% ethanol, 22% acetonitrile and 6% 1,2-dichloroethane.

[0036] Add 1g of oil to be tested into the organic phase medium system, and mix well. The prepared CNTs / SiO 2 -The MIPs modified electrode is inserted into the electrochemical medium system, and the detection limit of 3-MCPD in oil can reach 8.2×10 - 18 mol / L (S / N=3), the average recovery rate of 3-MCPD was 103.5%.

Embodiment 3

[0038] The same method as in Example 1 was adopted, except that the organic phase medium system used 66% ethanol, 24% acetonitrile and 10% 1,2-dichloroethane.

[0039] Add 1g of oil to be tested into the organic phase medium system, and mix well. The prepared CNTs / SiO 2 -The MIPs modified electrode is inserted into the electrochemical medium system, and the detection limit of 3-MCPD in oil can reach 6.3×10 - 18 mol / L (S / N=3), the average recovery rate of 3-MCPD was 96.9%.

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PUM

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Abstract

The invention provides a 3-MCPD detection method and device based on a surface molecular imprinting technique; to-be-tested grease and an organic phase medium system are mixed to form an electrochemical medium system, and a CNTs / SiO2-MIPs modified electrode directly detects the content of 3-MCPD in the electrochemical medium system, wherein the organic phase medium system includes a polar or weakpolar organic solvent. Compared with the prior art having too complex pretreatment process of the to-be-tested grease and low processing efficiency during detection of the 3-MCPD, the 3-MCPD detectionmethod and device based on the surface molecular imprinting technique have high detection efficiency and low detection limit.

Description

technical field [0001] The present invention relates to biological analysis detection technology, more specifically, to a 3-MCPD detection method and device based on surface molecular imprinting technology. Background technique [0002] 1,3-dichloro-2-propanol (abbreviation: 3-MCPD) is a general term for a series of homologues and isomers formed by a class of compounds produced by replacing the hydroxyl group on glycerol with chloride ions , Widely present in various foods such as grains, meat products, dairy products, baked foods, cooked foods, pickled products, edible oils, soups and drinking water. Because 3-MCPD is a non-genotoxic carcinogen, it can cause increased kidney body ratio, renal tubular hyperplasia and degeneration, and can reduce sperm count, sperm activity and survival rate, so it has been approved by the Joint Expert Committee on Food Additives (JECFA) Listed as a priority evaluation item, it has attracted the attention of domestic health scholars. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 陶莎李肖林高万林仲贞张国锋于丽敏
Owner CHINA AGRI UNIV