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Method for promoting germination of seeds of cremastra appendiculata

A rhododendron orchid and seed technology, applied in the field of plant tissue culture, can solve the problems of incomplete embryonic development, difficulty in sexual reproduction, difficulty in large-scale production, etc., and achieve the goal of reducing the damage rate, increasing the effect of disinfection, and reducing the pollution rate Effect

Inactive Publication Date: 2018-04-06
秦素梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although artificial pollination can make it bear fruit, it is very difficult to reproduce sexually because of obstacles such as incomplete development of the embryo and inhibition of the seed coat.
Although pseudobulbs can be used for artificial planting, the reproduction coefficient is extremely low and it is difficult to achieve large-scale production
At present, the research on Rhododendron orchid mainly focuses on chemical composition analysis, pharmacology and toxicology research, tissue culture and rapid propagation, etc. There are few reports on how to break through the problem of seed germination in sexual reproduction.

Method used

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  • Method for promoting germination of seeds of cremastra appendiculata
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Examples

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Embodiment 1

[0029] A method for promoting germination of rhododendron orchid seeds, the method comprising the following steps:

[0030] (1) Seed selection: choose rhododendron orchid capsules of different plants and cross-pollination and a growth period of 180 days;

[0031] (2) Seed surface treatment: After picking Rhododendron orchid capsules, wash them with running water to clean the surface, then place them in an ultra-clean workbench, soak them in 75% alcohol for 2 minutes, wash them with sterile water for 3 times, and then use plant disinfectant Soak for 5 minutes, and finally wash with sterile water for 3 times, and dry the surface moisture with anhydrous filter paper; the plant disinfectant used is made by mixing the following raw materials in parts by weight: 0.05 parts of acetylshikonin, 5 parts of aloe juice, oleander 0.1 parts of leaf extract, 2 parts of Tween-60, 75 parts of water;

[0032] (3) Seed disinfection: cut open the capsule longitudinally with a scalpel, shake out ...

Embodiment 2

[0035] A method for promoting germination of rhododendron orchid seeds, the method comprising the following steps:

[0036] (1) Seed selection: select rhododendron orchid capsules of different plants and cross-pollination and a growth period of 190 days;

[0037] (2) Seed surface treatment: After picking Rhododendron orchid capsules, rinse the surface with running water, then place it in a super-clean workbench, soak it with 75% alcohol for 2.5 minutes, wash it with sterile water for 4 times, and then sterilize it with plants solution for 8 minutes, and finally washed 4 times with sterile water, and dried the surface moisture with anhydrous filter paper; the plant disinfectant used was mixed with the following raw materials in parts by weight: 0.06 parts of acetylshikonin, 6 parts of aloe juice, bamboo 0.15 parts of peach leaf extract, 3 parts of Tween-60, 85 parts of water;

[0038] (3) Seed disinfection: cut open the capsule longitudinally with a scalpel, shake out the Rhod...

Embodiment 3

[0041] A method for promoting germination of rhododendron orchid seeds, the method comprising the following steps:

[0042] (1) Seed selection: choose rhododendron orchid capsules with different plants and cross-pollination and a growth period of 200 days;

[0043] (2) Seed surface treatment: After picking Rhododendron orchid capsules, rinse the surface with running water, then place it in an ultra-clean workbench, soak it with 75% alcohol for 3 minutes, wash it with sterile water for 5 times, and then use plant disinfectant Soak for 10 minutes, and finally wash 5 times with sterile water, and dry the surface moisture with anhydrous filter paper; the plant disinfectant used is made by mixing the following raw materials in parts by weight: 0.08 parts of acetylshikonin, 8 parts of aloe juice, oleander 0.2 parts of leaf extract, 4 parts of Tween-60, 100 parts of water;

[0044] (3) Seed disinfection: cut open the capsule longitudinally with a scalpel, shake out the Rhododendron ...

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Abstract

The invention provides a method for promoting the germination of Rhododendron orchid seeds, and belongs to the technical field of plant tissue culture. The method includes the steps of seed selection, seed surface treatment, seed disinfection and seed culture. The seed surface treatment and seed disinfection mainly use plant disinfectant for soaking. The active ingredients of the plant disinfectant are acetylshikonin, aloe juice, and oleander leaf extract; okra juice and moringa are added to the germination medium. Leaf juice serves as nutrients and NAA and GGR6 as auxins. The method of the invention can cause the seeds of Rhododendron to germinate early and improve the seed germination rate.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for promoting germination of rhododendron orchid seeds. 【Background technique】 [0002] Rhododendron orchid belongs to the Orchidaceae Rhododendron orchid, a perennial medicinal herb. Its dried pseudobulb is used as medicine. It can be used externally to treat snake bites and skin burns, and it can be used internally to fight liver cancer and breast cancer. Due to people's unrestrained excavation of rhododendron orchid medicinal materials, its wild resources are on the verge of exhaustion. Under natural conditions, rhododendron orchids can hardly pollinate and set fruit. Although artificial pollination can make it fruit, it is very difficult to reproduce sexually because of obstacles such as incomplete embryo development and seed coat inhibition. Although pseudobulbs can be used for artificial planting, the reproduction coefficient is extremely low, maki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/08A01C1/00A01H4/00A01N65/42A01N65/08A01N35/06A01P3/00A01P1/00
CPCA01C1/08A01C1/00A01H4/001A01H4/008A01N35/06A01N65/00A01N65/08A01N65/42
Inventor 秦素梅
Owner 秦素梅
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