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Tissue culture rapid propagation method of cymbidium floribundum

A technique for tissue culture, rapid propagation and multiflora, which is applied in the field of plant tissue culture, can solve the problems of multiflora shoot tips being susceptible to bacteria, being vulnerable to damage, and being sensitive to chemical disinfectants, so as to improve the induction differentiation rate and shorten the culture. cycle, the effect of improving quality

Inactive Publication Date: 2018-02-23
秦素梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some scholars have also studied the method of using the stem tips of Orchid multiflora as explants for tissue culture to obtain Orchid multiflora seedlings, but the stem tips of Orchid multiflora are easy to be infected with bacteria, and the stem tips are tender and sensitive to chemical disinfectants. , vulnerable to damage, resulting in low proliferation and differentiation rates during the culture process; in addition, due to the lack of related research, no medium that is more suitable for the growth of shoot tip differentiated tissues has been found, resulting in long culture periods and poor culture effects during tissue culture. unsatisfactory

Method used

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  • Tissue culture rapid propagation method of cymbidium floribundum
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Examples

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Embodiment 1

[0029] A method for tissue culture and rapid propagation of Cymbidium includes sterilization, induction culture, subculture and strong seedling culture of explants. The specific steps are:

[0030] (1) Disinfection of explants: Collect the stem tip of the multiflora orchid as explants. The explants are 2cm-4cm in length. First rinse with running water, then soak in 75% alcohol for 1 min, then peel off the surface part. The leaves are soaked in a plant disinfectant for 8 minutes and then part of the leaves are peeled off, and then soaked in the plant disinfectant for 5 minutes, rinsed with sterile water twice, and finally transferred to the ultra-clean workbench, peeled off under the dissecting mirror Remove the remaining leaves to expose the growing points, and use a scalpel to cut the stem tip meristem with a size of 2-5mm; the plant disinfectant is made by mixing the following raw materials: 0.05 parts of acetyl shikonin, 5 parts of aloe juice, 0.1 part of oleander leaf extract...

Embodiment 2

[0035] A method for tissue culture and rapid propagation of Cymbidium includes sterilization, induction culture, subculture and strong seedling culture of explants. The specific steps are:

[0036] (1) Disinfection of explants: Collect the stem tip of the multiflora orchid as explants. The explants are 2cm-4cm in length. First rinse with running water, then soak in 75% alcohol for 1 min, then peel off the surface part. The leaves are soaked in plant disinfectant for 12 minutes and then part of the leaves are peeled off, and then soaked in the plant disinfectant for 8 minutes, rinsed with sterile water 3 times, and finally moved into the ultra-clean workbench, peeled off under the dissecting mirror Remove the remaining leaves to expose the growing points, and use a scalpel to cut the stem tip meristem with a size of 2-5mm; the plant disinfectant is made by mixing the following raw materials: 0.06 parts of acetyl shikonin, 6 parts of aloe juice, 0.15 parts of oleander leaf extract,...

Embodiment 3

[0041] A method for tissue culture and rapid propagation of Cymbidium includes sterilization, induction culture, subculture and strong seedling culture of explants. The specific steps are:

[0042] (1) Disinfection of explants: Collect the stem tip of the multiflora orchid as explants. The explants are 2cm-4cm in length. First rinse with running water, then soak in 75% alcohol for 1 min, then peel off the surface part. The leaves are then soaked in plant disinfectant for 15 minutes and then part of the leaves are peeled off, and then soaked in the plant disinfectant for 10 minutes, rinsed with sterile water 4 times, and finally moved into the ultra-clean workbench, peeled under the dissecting mirror Remove the remaining leaves to expose the growing points, and cut 2-5mm apex meristems with a scalpel; the plant disinfectant is made by mixing the following raw materials: 0.08 parts of acetyl shikonin, 8 parts of aloe juice, 0.2 parts of oleander leaf extract, 4 parts of Tween-60, 1...

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Abstract

The invention provides a tissue culture rapid propagation method of cymbidium floribundum, belonging to the technical field of plant tissue culture. The tissue culture rapid propagation method comprises the steps of disinfection of explants, induced culture, subculture and strong seedling culture and the like, wherein the disinfection of the explants adopts a matched disinfection mode of alcohol disinfection and plant disinfecting liquid with the effective components of acetylshikonin, aloe juice and oleander leaf extract; and moringa oleifera leaf juice and lycium barbarum leaf juice are added in culture mediums used in all culture stages to be taken as nutritional agents. According to the tissue culture rapid propagation method provided by the invention, the damage to the explants is slight; and by adoption of the formula of the culture medium provided by the invention, the induced differentiation rate, the multiplication coefficient of the explants and the rooting rate of the cymbidium floribundum can be obviously increased, the culture period can be shortened and the quality of the tissue culture seedlings of the cymbidium floribundum can be improved.

Description

【Technical Field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of multiflora orchids. 【Background technique】 [0002] Cymbidium floribundum (Cymbidium floribundum) is an epiphytic plant belonging to the genus Orchidaceae, which belongs to Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. It is a secondary protected plant in my country. The multiflora orchid is mainly distributed in Yunnan, Guangxi, Guangdong, Fujian and other provinces south of the Yangtze River in my country; it is born in forests at an altitude of 100-3300m, on forest edge trees, or on rock walls along valleys. The leaves are leathery, shiny, and strong in drought and cold resistance; the flowers have a fragrance, a longer flowering period, and a variety of colorful flowers. They have high ornamental value and are one of the more ideal potted flowers. [0003] The b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N65/42A01N37/02A01P1/00
CPCA01H4/001A01H4/008A01N37/02A01N65/00A01N65/42
Inventor 秦素梅
Owner 秦素梅
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