Tissue culture method for avoiding crown inclination of cunninghamia lanceolata test-tube plantlets

A technique for test-tube seedlings and fir, which is applied in the field of tissue culture to avoid partial crowns of Chinese fir test-tube seedlings, and can solve problems such as changing the growth habit of Chinese fir, loss of balance of contents, and partial crowns of test-tube seedlings, so as to avoid the occurrence of partial crowns in tissue culture seedlings , control the occurrence of diseases, and the effect of low cost

Inactive Publication Date: 2018-04-10
福建三明林业学校
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Problems solved by technology

However, in the tissue culture process, due to long-term human interference with the addition of hormones to change the growth habits of Chinese fir, the contents of Chinese fir are out of balance, resulting in partial crowns of test tube seedlings

Method used

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Embodiment Construction

[0034] The implementation of the present invention will be described in detail below in conjunction with specific examples, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention.

[0035] Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the reagents and products used are also commercially available. The sources, trade names and, where necessary, listing of constituents of reagents used are indicated the first time they appear.

[0036] Embodiments of the invention are:

[0037] A method for cultivating seedlings of 2.5 generation fir excellent trees in Yangkou Forest Farm, Fujian, comprising the following steps:

[0038] (1) Acquisition of explants: At 4:00 pm on three consecutive sunny days in early May, select the 2.5-generation Chinese fir Youshu clone saplings, and take the s...

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Abstract

The invention relates to the field of plant tissue culture, provides a tissue culture method for avoiding crown inclination of cunninghamia lanceolata test-tube plantlets, and solves the defect of crown inclination of test-tube plantlets cultured in the prior art. The method comprises the following steps: (1) obtaining explants; (2) performing proliferation culture; (3) performing seedling strengthening culture; (4) performing rooting culture; (5) hardening seedlings: hardening the seedlings under the condition of a 60% shading degree, when root systems change colors, keeping the humidity of agreenhouse to be 70% or more, opening a cover, and hardening the seedlings for 3 days; and (6) transplanting the test-tube plantlets: when transplantation is performed, rinsing the rooting seedlingswith fresh water, draining off water, soaking the treated seedlings into a 0.2% potassium permanganate aqueous solution for 5min, lifting the seedlings, rinsing the seedlings with fresh water for 3 times, putting the rinsed seedlings into seedling raising mixed matrix nutrient bags, performing culture, and when the heights of the seedlings cultured in containers reach 30cm, enabling the seedlingsto leave a nursery for afforestation.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a tissue culture method for avoiding partial crowns of Chinese fir test-tube seedlings. Background technique [0002] Chinese fir (Cunninghamia lanceolata (Lamb) Hook.) belongs to Taxodiaceae, also known as sand tree, sand tree, etc. Arbor, straight grain, fine wood structure, light reddish-brown heartwood, light material, corrosion-resistant and moth-resistant, easy to process, not easy to warp. Widely used in construction, bridges, poles, shipbuilding, furniture, etc. It has a wide range of distribution in my country, and is the most widely cultivated in the Yangtze River Basin and south of the Qinling Mountains. It grows fast, has a short operating cycle, and has high economic value. It is the main fast-growing timber tree species in southern my country. Medicinal function: expelling wind and relieving pain, dissipating blood stasis and stopping bleeding. For chronic bro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40Y02A40/22
Inventor 黄云鹏陈绍煌范繁荣王邦富
Owner 福建三明林业学校
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