Extraction method of ground beeltle polysaccharide

An extraction method and a technology for the extraction of A. vulgaris are applied in the field of extraction of polysaccharides from B. vulgaris, which can solve problems such as insufficient polysaccharide purity, and achieve the effects of high decolorization capacity, fast extraction rate, and complete degreasing.

Inactive Publication Date: 2018-05-08
海盐县凌特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purity of the polysaccharide obtained by this extraction method is not enough, and it contains miscellaneous proteins and small molecular substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for extracting wood beetle polysaccharides, comprising degreasing, ultrasonic-microwave extraction, and decolorization, the specific steps of which are:

[0015] Degreasing: Crush the wood beetle into a coarse powder, add a mixed solvent 5-6 times the weight of the wood beetle, the volume ratio of petroleum ether and acetone in the mixed solvent is 1:0.7-0.8, at a temperature of 40-50°C and a power of 100- Ultrasonic degreasing at 150W for 35-42min, filtering, drying the filter residue at low temperature and crushing to make defatted wood beetle powder. Cytosine;

[0016] Ultrasonic-microwave extraction: Soak the defatted ground beetle powder in distilled water 20-30 times the weight, then add multifunctional active protein, and then treat it with an ultrasonic microwave extractor at 70-80°C for 40-50min, cool to room temperature, and Centrifuge at 4000-5000r / min for 12-16min, concentrate the supernatant to 1 / 4-1 / 2 of the original volume, put it into a dialysi...

Embodiment 2

[0019] A method for extracting wood beetle polysaccharides, comprising degreasing, ultrasonic-microwave extraction, and decolorization, the specific steps of which are:

[0020] Degreasing: Crush the wood beetle into a coarse powder, add a mixed solvent 6 times the weight of the wood beetle, the volume ratio of petroleum ether and acetone in the mixed solvent is 1:0.7, ultrasonic degrease at a temperature of 50°C and a power of 100W for 42 minutes, filter, The filter residue is dried at low temperature and pulverized to make defatted wood beetle powder. The mixed solvent contains 0.025% amphotericin by solvent weight and 0.011% 5-fluorocytosine by solvent weight;

[0021]Ultrasonic-microwave extraction: Soak the defatted ground beetle powder in distilled water 30 times its weight, then add multifunctional active protein, and then treat it with an ultrasonic microwave extractor at 70°C for 50min, cool to room temperature, and centrifuge at 5000r / min for 12min , Concentrate the ...

Embodiment 3

[0024] A method for extracting wood beetle polysaccharides, comprising degreasing, ultrasonic-microwave extraction, and decolorization, the specific steps of which are:

[0025] Degreasing: crush the wood beetle into a coarse powder, add a mixed solvent 5.5 times the weight of the wood beetle, the volume ratio of petroleum ether and acetone in the mixed solvent is 1:0.75, ultrasonic degrease at a temperature of 45°C and a power of 120W for 40 minutes, filter, The filter residue is dried at low temperature and pulverized to make defatted wood beetle powder. The mixed solvent contains 0.024% amphotericin by solvent weight and 0.012% 5-fluorocytosine by solvent weight;

[0026] Ultrasonic-microwave extraction: Soak defatted ground beetle powder in 25 times the weight of distilled water, then add multifunctional active protein, and then treat it with ultrasonic microwave extractor at 75°C for 45min, cool to room temperature, and centrifuge at 4500r / min for 14min , Concentrate the ...

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Abstract

The invention discloses an extraction method of a ground beeltle polysaccharide, wherein the extraction method comprises the specific steps: crushing ground beeltle into a coarse powder, carrying outultrasonic defatting, filtering, drying the filter residue, and crushing to prepare a defatted ground beeltle powder; soaking the defatted ground beeltle powder in water, treating with an ultrasonic and microwave extraction instrument, centrifuging, concentrating the supernatant, dialysing, carrying out ethanol precipitation, centrifuging, removing proteins by a repeated freeze thawing method anda Sevage method, dialysing the supernatant, concentrating, and freeze-drying, to obtain ground beeltle polysaccharide; loading a chromatographic column with NKA-9 macroporous adsorption resin, treating a ground beeltle polysaccharide solution, collecting an eluate, concentrating, drying, and thus obtaining the ground beeltle polysaccharide. The extraction method is simple and feasible, the extraction rate is fast, the extraction rate and purity of the polysaccharide are high, the amount of water and a solvent can be reduced, the original structure and activity of the polysaccharide can be preserved, the energy consumption is reduced, and the method is more suitable for development direction of industrialized production.

Description

technical field [0001] The invention relates to the technical field of deep processing of natural products, in particular to a method for extracting wood beetle polysaccharides. Background technique [0002] Ground soft-shelled beetle has another name called ground soft-shelled turtle, ground soft-worm, is the insect ground soft-shelled turtle ( Eupolyphaga sinesis Walker) or Jidi softshell turtle (Eupolyphaga sinesis ( Boleny )) The dried body of the female worm is mainly produced in Fujian, Hebei, Guangxi, Guangdong, Shandong and other places. It is cold in nature and salty in taste, and it is a traditional Chinese medicine for promoting blood circulation and removing blood stasis. There are records about its medicinal use in "Compendium of Materia Medica" written by Li Shizhen in the Ming Dynasty. It is a traditional insect medicine for promoting blood circulation and removing blood stasis. It can regulate lipid metabolism, resist lipid peroxidation, and protect endot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 董珊珊
Owner 海盐县凌特生物科技有限公司
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