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Normal Temperature Preservation Method for Human Genome DNA Samples

A technology of room temperature preservation and genomics, applied in the field of room temperature preservation of genetic samples, can solve the problems of difficult personal preservation, harsh implementation conditions, fixed-point storage, etc., and achieve the effect of convenient sample sealing, ensuring stability and integrity, and easy production

Active Publication Date: 2018-11-30
浙江植根生物材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems of harsh implementation conditions, high cost, need for professional equipment, fixed-point storage, and difficulty in personal preservation in the conventional human genome DNA sample preservation process, and provides the human genome DNA sample room temperature preservation method of the present invention for this purpose , this method is relatively simple to operate, low in cost, easy to store samples, and can be stored at room temperature for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1. Preparation and storage of genomic DNA samples from human oral mucosal epithelial cells

[0013] 1. Collection of human oral mucosal epithelial cells

[0014] Rinse your mouth with clean water several times before sampling, and then use an oral swab to repeatedly scrape the inner wall of the oral cavity several times, so that the oral mucosal epithelial cells are carried on the oral swab.

[0015] 2. Lyse cells

[0016] Cut the material containing oral mucosal epithelial cells and place it in an Eppendorf (EP) tube, add 400 μL of cell lysate to the EP tube, and mix well until there are no obvious cell clusters. The formula of cell lysate is: 10 mmol / L Tris-HCl (pH8.0), 30 mmol / L EDTA (pH8.0), 0.5% SDS, RNase A (20 μg / mL).

[0017] 3. Treatment with chaotropic agent to release genomic DNA

[0018] Add the chaotropic agent sodium bromate with a final concentration of 1 mol / L to the EP tube, and mix well.

[0019] 4. Remove protein

[0020] Add an equal vol...

Embodiment 2

[0031] Example 2. Genomic DNA sample preparation and storage thereof with human blood

[0032] 1. Human blood sample collection

[0033] Collect 0.5-2ml of anticoagulated blood or prepare blood spots. This blood sample can be kept frozen at -80 ℃ for at least 2 years without deterioration.

[0034] 2. Lysing red blood cells

[0035] Add 400 μL of erythrocyte lysate to 200 μL of anticoagulated blood, mix well, centrifuge at 2000 g for 10 min, and discard the supernatant. If there are still more red blood cells remaining, the lysis can also be repeated once.

[0036] Subsequent operations are consistent with steps (2)-(9) of Example 1.

Embodiment 3

[0037] Example 3. Preparation of Genomic DNA Sample and Its Sealing with Human Hair Follicles

[0038] Pull out 3 hairs with clean tweezers, wash the hair and the attachments of the hair follicles at the root with distilled water, then cut 1 cm from the root of the hair, immerse the hair follicles at the root into a centrifuge tube containing 400 μl of cell lysate, remove the hair after the cells are fully lysed and other impurities, the follow-up operations are consistent with steps (2)-(9) of Example 1.

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Abstract

The invention discloses a normal temperature preservation method of human genome DNA samples. A prepared and extracted human genome DNA solution is evenly mixed with a melted low melting-point agarosesolution. After drying, a human genome DNA powder sample capable of being stored at room temperature is prepared. The powder sample is placed in a container and sealed, and then is placed in a light-tight container or environment. The normal temperature preservation method is suitable for normal temperature preservation of the human genome DNA samples.

Description

technical field [0001] The invention relates to a method for storing gene samples at room temperature, in particular to a method for storing human genome DNA samples at room temperature. Background technique [0002] Human genomic DNA refers to all the DNA that makes up the human genome, and refers to all the genetic information that can be passed from one generation to the next. Human genome DNA contains all the genetic information of the human body and is the material basis for heredity and species continuation. It makes the offspring appear similar to the parents. Preserving human genome DNA samples means preserving all life genetic information of the human body. With the continuous advancement of molecular biology technologies such as genome sequencing, scientists have been able to analyze and interpret the genetic information and life codes of genome DNA. Human genome DNA samples have been and will be more widely used in various gene cloning, diseases Diagnosis, medic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
CPCC12N15/10
Inventor 陈礼平王海英
Owner 浙江植根生物材料科技有限公司
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