A kind of dendrobium extract and its application in the preparation of whitening sunscreen
A Dendrobium orchid extract technology, applied in the field of natural plant extraction and cosmetics, can solve the problems of not fully exploiting the output value of the orchid industry, restricting the healthy and sustainable development of the orchid industry, and achieving a strong tyrosinase inhibitory activity and its effect on UV absorption
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Embodiment 1
[0025] (1) Dendrobium orchid is dried, pulverized to 20-40 order, obtains material 1.0kg;
[0026] (2) Add an ethanol solution (1.0g / mL, 500mL) of 1-butyl-3-methylimidazolium bromide to the material obtained in step (1), and after airtight infiltration for 1 hour, add a volume fraction of 60% Ethanol solution, heated to reflux temperature, reflux extraction for 2 hours, filtered, collected filtrate, concentrated to obtain crude extract;
[0027] (3) After diluting the crude extract obtained in step (2) with an appropriate amount of water, adding hydrochloric acid to adjust the pH to 2.0-5.0, extracting twice with ether, combining the organic phases, concentrating under reduced pressure, and drying to obtain the dendrobium extract Material 32g (hereinafter referred to as product A).
Embodiment 2
[0029] (1) Dendrobium orchid is dried, pulverized, obtains material 1.0kg;
[0030] (2) Add an ethanol solution (0.5g / mL, 600mL) of 1-butyl-3-methylimidazolium chloride salt to the material obtained in step (1), and after airtight infiltration for 2 hours, add a volume fraction of 70% Ethanol solution, heated to reflux temperature, reflux extraction for 3 hours, filtered, collected filtrate, concentrated to obtain crude extract;
[0031] (3) After the crude extract obtained in step (2) is diluted with an appropriate amount of water, after adding hydrochloric acid to adjust the pH to 2.0-5.0, extract 3 times with ether, combine the organic phases, concentrate under reduced pressure, and dry to obtain the Dendrobium orchid extract Material 33g (hereinafter referred to as product B).
Embodiment 3
[0033] (1) Dendrobium orchid is dried, pulverized to 20-40 order, obtains material 1.0kg;
[0034] (2) adding an ethanol solution with a volume fraction of 60% to the material obtained in step (1), heating to reflux temperature, reflux extraction for 2 hours, filtering, collecting the filtrate, and concentrating to obtain a crude extract;
[0035] (3) After diluting the crude extract obtained in step (2) with an appropriate amount of water, adding hydrochloric acid to adjust the pH to 2.0-5.0, extracting twice with ether, combining the organic phases, concentrating under reduced pressure, and drying to obtain the dendrobium extract Thing 22g (hereinafter referred to as product C).
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