Leech extract and preparation method and application thereof

Abstract

The invention discloses a leech extract preparation method. The method is characterized in that water is added in leech and the materials are homogenized, and steps of controllable enzymatic hydrolysis, deposition with an organic solvent, ultrafiltration, and anion exchange chromatography are carried out to prepare the leech extract. The leech extract can be used for preparing the medicines havingthe anticoagulant effect, thrombolysis effect, platelet aggregation inhibition effect and nerve protection effect. From technical requirements of modernization of traditional Chinese medicines, a controllable enzymatic hydrolysis technology is employed for extracting a peptide component in leech, combining with a biopharmaceutical technology, an ultrafiltration technology and a gel chromatographyseparating technology can be employed for further purifying, and the extract having higher purity and stronger activity than that of the traditional leech extract can be obtained. The method breakthroughs the bottleneck of the traditional animal drugs on extraction and separating aspects, and solves the problems of large amount and fishy smell of used raw powder.

Description

technical field [0001] The present invention relates to a kind of leech extract and its preparation method and application technical background [0002] The effect of leech is to break blood, expel blood stasis, and eliminate symptoms. It can be used for lumps in the abdomen, blood stasis, amenorrhea, bruises, thrombocytosis, and cerebral hemorrhage. Leeches were first recorded in "Shen Nong's Materia Medica": "The main purpose is to drive away bad blood, blood stasis, moon closure, accumulation of broken blood, no children, and water passages." Salty and bitter, in order to remove blood accumulation, it is the medicine of the liver meridian blood, so it can dredge the liver meridian and gather blood." [0003] As a traditional Chinese medicine, leeches are widely used clinically, but the active ingredients are not clear, and the literature reports that proteins and polypeptides are the main active ingredients. The ambiguity of active ingredients is an inherent problem ...

AI Technical Summary

Problems solved by technology

[0003] As a traditional Chinese medicine, leeches are widely used clinically, but the active ingredients are not clear, and the literature reports that proteins and polypeptides are the main active ingredients.

The ambiguity of active ingredients is an inherent problem of animal medicinal materials. Traditional extraction and concentration methods may cause some ingredients to decompose, destroy, or fail to extract, which will easily cause the loss of active ingredients, so it is not suitable for animal medicinal materials.