Method for preparing flocculant by utilizing fast-growing bacillus strain

A flocculant and bacillus technology, applied in the field of adsorbent preparation, can solve the problems of low flocculation activity, high cost and low yield, and achieve the effects of no toxic side effects, stable performance and high flocculation rate

Active Publication Date: 2018-05-29
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of microbial flocculant-producing bacteria have been screened from soil, activated sludge, domestic sewage, and various industrial wastewater environments. Among them, Aspergillus sojae AJ7002 and Takagi which produce microbial flocculant AJ7002 discovered by Nakamura et al. Paecilomyces producing PF101 and Rhodococcus erythrophylla producing NOC-1 found by Kurane et al. These microbial flocculants have the advantages of environmental friendliness and no secondary pollution compared with traditional polymer chemical flocculants, but due to Some substrates required for the production of bioflocculants, such as sucrose, glucose, and fructose, are too expensive, low in yield, and low in flocculation activity, making it difficult to scale up their application and industrialization. The promotion application is still quite different

Method used

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  • Method for preparing flocculant by utilizing fast-growing bacillus strain

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Effect test

Embodiment 1

[0032] A kind of method utilizing quick-growing bacillus bacterial strain to prepare flocculant, comprises the following steps:

[0033] a) Inoculate the fast-growing bacillus strain on the solid medium for cultivation, add sterile water to make the fast-growing bacillus strain; the culture temperature of the fast-growing bacillus strain is 25° C., and the cultivation time is 36 hours, and the ratio of the sterile water and the solid medium The volume ratio is 1.0:1;

[0034] b) Inoculate the fast-growing bacteria seed liquid into the liquid medium for fermentation and culture to make the fermentation bacterial liquid; the inoculation concentration of the fast-growing bacteria seed liquid is to inoculate 1.5mL of the fast-growing bacteria seed liquid for every 100mL liquid medium, and the culture temperature is 23°C. The culture time is 3 days, and the shaker speed is 100rpm;

[0035] c) Add modified bentonite to the fermented bacteria liquid and continue to ferment and culti...

Embodiment 2

[0040] A kind of method utilizing quick-growing bacillus bacterial strain to prepare flocculant, comprises the following steps:

[0041] a) Inoculate the fast-growing bacillus strain on the solid medium for cultivation, add sterile water to make the fast-growing bacillus strain; the culture temperature of the fast-growing bacillus strain is 27 ℃, and the cultivation time is 42 hours, the ratio of sterile water and solid medium The volume ratio is 1.25:1;

[0042] b) Inoculate the fast-growing bacilli seed liquid into the liquid medium for fermentation and culture to make the fermented bacterial liquid; the inoculation concentration of the fast-growing bacillus bacterial seed liquid is to inoculate 2.0mL of the fast-growing bacillus bacterial seed liquid per 100mL liquid medium, and the culture temperature is 25°C. The culture time is 4 days, and the shaker speed is 130rpm;

[0043] c) Add modified bentonite to the fermented bacteria liquid and continue to ferment and cultivat...

Embodiment 3

[0048] A kind of method utilizing quick-growing bacillus bacterial strain to prepare flocculant, comprises the following steps:

[0049] a) Inoculate the fast-growing bacillus strain on the solid medium for cultivation, add sterile water to make the fast-growing bacillus strain; the culture temperature of the fast-growing bacillus strain is 30° C., and the cultivation time is 48 hours, the ratio of sterile water and solid medium The volume ratio is 1.5:1;

[0050] b) Inoculate the fast-growing bacilli seed liquid into the liquid medium for fermentation and culture to make the fermented bacterial liquid; the inoculation concentration of the fast-growing bacillus bacterial seed liquid is to inoculate 2.5mL of the fast-growing bacillus bacterial seed liquid per 100mL liquid medium, and the culture temperature is 27°C. The culture time is 5 days, and the shaker speed is 160rpm;

[0051] c) Add modified bentonite to the fermented bacteria liquid and continue to ferment and cultiva...

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Abstract

The invention belongs to the technical field of flocculant preparation. The invention discloses a method for preparing a flocculant by utilizing a fast-growing bacillus strain. The method includes thesteps of strain culture, fermentation culture, modified bentonite sedimentation, drying and the like, wherein the fast-growing bacillus strain is the fast-growing bacillus strain Celeribacter sp.GHF1031 obtained by separating and purifying sludge spit by ruditapes philippinarum. The flocculant prepared through the method is high in flocculation rate, stable in performance, free of toxic and sideeffects, high in safety, easy to produce and low in cost, and can be used as a wastewater flocculant.

Description

technical field [0001] The invention relates to the technical field of adsorbent preparation, in particular to a method for preparing a flocculant by using fast-growing bacteria strains. Background technique [0002] Flocculants are one of the commonly used agents in water treatment technology. In recent years, the most widely used traditional high-molecular polymer flocculants have been found to have potential problems that threaten human health and destroy the ecological environment. Research on new environmentally friendly and pollution-free flocculants has become a hot issue in water treatment research. Most of them are microbial flocculants. Bioflocculants are a class of polymers with biodegradable functions, including polysaccharides, lipids and proteins secreted by microorganisms with such special functions. Because of its safety and non-pollution to the environment, it is valued by many scientific researchers at home and abroad. At present, a variety of microbial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/20C12R1/01
CPCC12N1/20C12P19/04
Inventor 穆军崔霞杨桥阳广凤陈庆国
Owner ZHEJIANG OCEAN UNIV
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