A kind of method that utilizes rhodobacter albicans strain to prepare flocculant

A flocculant and bacillus technology is used in the field of preparing flocculants by using Rhodobacter albicans strains, which can solve the problems of low flocculation activity, high cost, low yield and the like, and achieve the effects of no toxic side effects, high safety and high flocculation rate.

Active Publication Date: 2021-05-14
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of microbial flocculant-producing bacteria have been screened from soil, activated sludge, domestic sewage, and various industrial wastewater environments. Among them, Aspergillus sojae AJ7002 and Takagi which produce microbial flocculant AJ7002 discovered by Nakamura et al. Paecilomyces producing PF101 and Rhodococcus erythrophylla producing NOC-1 found by Kurane et al. These microbial flocculants have the advantages of environmental friendliness and no secondary pollution compared with traditional polymer chemical flocculants, but due to Some substrates required for the production of bioflocculants, such as sucrose, glucose, and fructose, are too expensive, low in yield, and low in flocculation activity, making it difficult to scale up their application and industrialization. The promotion application is still quite different

Method used

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  • A kind of method that utilizes rhodobacter albicans strain to prepare flocculant

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Experimental program
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Effect test

Embodiment 1

[0032] A kind of method utilizing rhodobacter albicans bacterial strain to prepare flocculant, comprises the following steps:

[0033] a) Inoculate the Rhodobacter albicans strain on a solid medium for cultivation, add sterile water to make the Rhodobacter albicans seed liquid; the culture temperature of the Rhodobacter albicans strain is 23°C, and the cultivation time is 35 hours, and the sterile water added The volume is 1.2 times that of the solid medium;

[0034] b) Inoculate the Rhodobacter albicans seed liquid into the liquid medium for fermentation and culture to make the fermentation bacterial liquid; the inoculation concentration of the Rhodobacter albicans seed liquid is to inoculate 1.5mL of the Rhodobacter albicans seed liquid for every 100mL liquid medium, and the culture temperature is 20°C, culture time is 3 days, shaker speed is 100rpm;

[0035] c) Add modified bentonite to the fermented bacteria liquid and continue to ferment and cultivate to obtain raw mater...

Embodiment 2

[0040] A kind of method utilizing rhodobacter albicans bacterial strain to prepare flocculant, comprises the following steps:

[0041] a) Inoculate the Rhodobacter albicans strain on a solid medium for cultivation, add sterile water to make the Rhodobacter albicans seed liquid; the culture temperature of the Rhodobacter albicans strain is 25°C, and the cultivation time is 42.5 hours, and the added sterile water The volume of the solid medium is 1.35 times;

[0042] b) Inoculate the Rhodobacter albicans seed liquid into the liquid medium for fermentation and culture to make the fermented bacterial liquid; the inoculation concentration of the Rhodobacter albicans seed liquid is to inoculate 2.0mL of the Rhodobacter albicans seed liquid for every 100mL liquid medium, and the culture temperature is 24°C, culture time is 4 days, shaker speed is 140rpm;

[0043] c) Add modified bentonite to the fermented bacteria liquid and continue to ferment and cultivate to obtain raw material l...

Embodiment 3

[0048] A kind of method utilizing rhodobacter albicans bacterial strain to prepare flocculant, comprises the following steps:

[0049] a) Inoculate the Rhodobacter albicans strain on a solid medium for cultivation, add sterile water to make the Rhodobacter albicans seed liquid; the culture temperature of the Rhodobacter albicans strain is 37° C., and the cultivation time is 50 hours, and the added sterile water The volume is 1.5 times that of solid medium;

[0050] b) Inoculate the Rhodobacter albicans seed liquid into the liquid medium for fermentation and culture to make the fermented bacterial liquid; the inoculation concentration of the Rhodobacter albicans seed liquid is to inoculate 2.5mL of the Rhodobacter albicans seed liquid for every 100mL liquid medium, and the culture temperature is 28°C, culture time is 5 days, shaker speed is 180rpm;

[0051] c) Add modified bentonite to the fermented bacteria liquid and continue to ferment and cultivate to obtain raw material l...

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Abstract

The invention belongs to the technical field of flocculant preparation. The invention discloses a method for preparing a flocculant by using Rhodobacter albicans strain, which comprises the steps of bacterial culture, fermentation culture, sedimentation of modified bentonite, drying and the like, wherein the Rhodobacter albicans strain is sludge spit out from Philippine clams Albirhodobacter sp. GHF1032, which was isolated and purified from R. albicans. The flocculant prepared by the method of the present invention has the characteristics of high flocculation rate, stable performance, no toxic and side effects, high safety, simple production and low cost, and can be used as a waste water flocculant.

Description

technical field [0001] The invention relates to the technical field of flocculant preparation, in particular to a method for preparing flocculants by using Rhodobacter albicans strains. Background technique [0002] Flocculants are one of the commonly used agents in water treatment technology. In recent years, the most widely used traditional high-molecular polymer flocculants have been found to have potential problems that threaten human health and destroy the ecological environment. Research on new environmentally friendly and pollution-free flocculants has become a hot issue in water treatment research. Most of them are microbial flocculants. Bioflocculants are a class of polymers with biodegradable functions, including polysaccharides, lipids and proteins secreted by microorganisms with such special functions. Because of its safety and non-pollution to the environment, it is valued by many scientific researchers at home and abroad. At present, a variety of microbial f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12N1/20C12R1/01
CPCC12N1/20C12P19/04
Inventor 穆军杨桥陈庆国冯丽娟阳广凤
Owner ZHEJIANG OCEAN UNIV
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