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Efficient microbial composite flocculant and preparation method thereof

A technology of composite flocculant and high-efficiency microorganisms, which is applied in the treatment of granular microbial carriers, chemical instruments and methods, biological water/sewage treatment, etc., and can solve problems such as unstable flocculation effects

Active Publication Date: 2021-12-31
重庆沐兰环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention intends to provide a high-efficiency microbial composite flocculant to solve the problem of unstable flocculation effect of existing microbial composite flocculants

Method used

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  • Efficient microbial composite flocculant and preparation method thereof
  • Efficient microbial composite flocculant and preparation method thereof
  • Efficient microbial composite flocculant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]A high-efficiency microbial composite flocculant, including 1 part of modified nano-ferric oxide and 100 parts of mixed bacterial liquid of Caulobacter crescentus and Klebsiella; the preparation process includes the preparation of mixed bacterial liquid, modified nano-si The preparation of ferric oxide and the preparation of high-efficiency microbial composite flocculant comprise the following steps:

[0034] 1. Preparation of activated bacterial liquid:

[0035] S1: Prepare medium: weigh 20g of glucose, 2g of peptone, 2g of potassium dihydrogen phosphate, 5g of dimethyl hydrogen phosphate, 0.2g of ammonium sulfate, 0.2g of magnesium sulfate heptahydrate, 0.5g of urea, and 0.5g of yeast extract, dissolve in In bacterial water, adjust the pH to 7.0, quantify the sterile water to 1000mL; sterilize at 121°C for 20min.

[0036] S2: Inoculation culture: inoculate the strains of C. crescentus and Klebsiella in S1 medium respectively, culture them in a shaker at 30°C and 150r / ...

experiment example 1

[0051] Experimental example 1: Flocculation effect of modified nano ferric oxide

[0052] Prepare 5g / L kaolin suspension, after stirring evenly, get 100ml respectively and put it in seven 100ml beakers, add 0.5ml10% calcium chloride solution, add flocculant according to the concentration of 10g / L respectively (comparative examples 1~6), Add sterile water as a blank control, stir rapidly for 1 min, and then stir slowly for 1 min after an interval of 2 min, and let it stand for 30 min. Take the supernatant and measure the absorbance at the wavelength λ=550 nm of the spectrophotometer. The calculation formula of flocculation rate:

[0053]

[0054] In the formula: FR is the flocculation rate; A is the absorbance of the water sample added with flocculant after standing; B is the absorbance of the water sample added with sterile water after standing. The experimental results are shown in Table 2.

[0055] Table 2 Comparison of flocculation rates of different modified nano-ferr...

experiment example 2

[0059] Experimental Example 2: Flocculation Effect of Klebsiella and Crescent Bacteria

[0060] According to 0.5%, 1%, 2%, and 4% of the mass volume of the test kaolin suspension, the Klebsiella bacterium liquid and the Crescent bacillus bacterium liquid were mixed according to a 1:1 ratio to obtain a mixed bacterium liquid. The flocculation rate of the mixed bacterial solution was determined according to the method shown in Experimental Example 1, and the experimental results are shown in Table 3.

[0061] Table 3 Comparison of flocculation rates of different bacterial solutions

[0062]

[0063] The experimental results show that when the addition amount of the two bacterial solutions in the microbial flocculant is too low (addition ratio ≤ 0.5%), the flocculation effect of the individual bacterial solution and the mixed bacterial solution is not significant, and when the addition ratio of the two bacterial solutions is When the concentration is 1% to 4%, the flocculatio...

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Abstract

The invention relates to the field of sewage treatment, and discloses an efficient microbial composite flocculant and a preparation method thereof. The efficient microbial composite flocculant is prepared from 1-10 parts of modified nano ferroferric oxide and 100 parts of mixed bacterium liquid of closterium and klebsiella; the modified nano ferroferric oxide is prepared by sequentially carrying out oleic acid modification and cation modification on nano ferroferric oxide. The nano ferroferric oxide subjected to two-step modification and a mixed bacterial solution of the closterium and the klebsiella are subjected to co-culturing, so that microorganisms and secretions are in full contact with ferroferric oxide particles, and the efficient microbial composite flocculant is formed. The efficient microbial composite flocculant has the advantages of high magnetism, high hydrophilicity, high stability, remarkable and stable flocculation effect, simple preparation process, mild reaction conditions, energy conservation, environmental protection, easiness in industrialization and the like, and can be used for various sewage treatments.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a high-efficiency microbial composite flocculant and a preparation method thereof. Background technique [0002] Traditional flocculants have a limited removal rate of suspended particles and other pollutants in the actual use process, and the follow-up mud-water separation process is longer, so that a larger sedimentation tank volume is required, resulting in increased initial investment costs and land occupation. increase in area. At present, composite flocculants are widely used, such as inorganic and organic composite flocculants, organic and organic composite flocculants, inorganic and microbial composite flocculants, organic and microbial composite flocculants, etc., but most flocculants have different effects after use. Problems, for example, the increase in sludge will lead to increased treatment costs, or will have different degrees of impact on the environment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34B01J20/06B01J20/28C02F101/20C02F101/30C02F1/52
CPCC02F3/34C02F3/348B01J20/06B01J20/28009B01J20/28011C02F2101/30C02F2101/20C02F2003/001C02F2001/007C02F1/5281Y02W10/10
Inventor 窦建军李雯蒋克梅程士兵
Owner 重庆沐兰环保科技有限公司
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