Fern extract for preventing and controlling saprolegniasis of Atlantic salar and application thereof

A kind of extract and fern technology, which is applied in the field of fern extract to prevent and control Atlantic salmon saprolegniasis, can solve the problems of injured tissue, parasitic necrotic tissue of hyphae, fry damage, etc., so as to inhibit the invasion, prevent infection, Mortality reduction effect

Active Publication Date: 2018-06-29
铜仁市万山区水产站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the process of raising seedlings, when a series of operations such as weighing, pouring tanks, dividing fish, and vaccinating are carried out, some fry may be damaged, and Saprolegnia will take the opportunity to enter, and the hyphae will parasitize necrotic tissue, causing the disease to occur. Saprolegniasis is A very common disease in the seedling raising period, which can occur throughout the seedling raising stage, often infects the injured tissue on the body surface, and forms a gray-white cotton-like covering

Method used

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  • Fern extract for preventing and controlling saprolegniasis of Atlantic salar and application thereof
  • Fern extract for preventing and controlling saprolegniasis of Atlantic salar and application thereof
  • Fern extract for preventing and controlling saprolegniasis of Atlantic salar and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 30kg Pteris fern, 27kg Osmanthus fragrans, 17kg crescent fern, 26kg black fern, 12kg Chestnut stalk golden powder fern are pulverized with an ultrafine pulverizer, and the crushed material is reflux extracted with methanol solution. The mass concentration of the methanol solution is 80%, the mass concentration of the methanol solution that refluxes for the second time is 90%, the mass concentration of the methanol solution that refluxes for the third time is 100%, each 50min, and then the reflux extract is decompressed with a rotary evaporator Concentrate, and alternately reduce the vacuum degree from -0.05MPa to -0.09MPa by reducing the pressure by 0.01MPa at a uniform speed within 10min and then maintaining it for 10min.

Embodiment 2

[0021] 35kg Pteris fern, 28kg Osmanthus fragrans, 18kg crescent fern, 27kg black fern, and 15kg Chestnut stalk golden powder fern are pulverized with an ultrafine pulverizer, and the pulverized matter is reflux extracted with methanol solution, and the reflux extraction is performed 3 times. The mass concentration of the methanol solution is 80%, the mass concentration of the methanol solution that refluxes for the second time is 90%, and the mass concentration of the methanol solution that refluxes for the third time is 100%, each 55min, and then the reflux extract is decompressed with a rotary evaporator Concentrate, and alternately reduce the vacuum degree from -0.05MPa to -0.09MPa by reducing the pressure by 0.01MPa at a uniform speed within 10min and then maintaining it for 10min.

Embodiment 3

[0023] 40kg Pteris fern, 29kg Osmanthus fragrans, 19kg crescent fern, 28kg black fern, and 18kg Chestnut stalk golden powder fern are pulverized with an ultrafine pulverizer, and the crushed material is reflux extracted with methanol solution, and the reflux extraction is performed 3 times. The mass concentration of the methanol solution is 80%, the mass concentration of the methanol solution that refluxes for the second time is 90%, and the mass concentration of the methanol solution that refluxes for the third time is 100%, each 60min, and then the reflux extract is decompressed with a rotary evaporator Concentrate, and alternately reduce the vacuum degree from -0.05MPa to -0.09MPa by reducing the pressure by 0.01MPa at a uniform speed within 10min and then maintaining it for 10min.

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Abstract

The invention relates to the technical field of culture of Atlantic salar, in particular to a fern extract for preventing and controlling saprolegniasis of Atlantic salar and application thereof. Thefern extract is prepared by the following steps of after multiple types of ferns and the usage amount are tested, selecting pteris multifida poir, dicranopteris pedata, pronephrium aspera, stenoloma chusana and onychium lucidum as extracting raw materials; crushing, refluxing, concentrating, and the like, so as to obtain the fern extract with obvious effect on saprolegniasis fungi and the component content. The fern extract has the advantages that when the fern extract is used for treating or preventing, the fern extract is matched with sodium chloride to form a salt compound, the injury to the fish body by the saprolegniasis fungi is inhibited, the death rate of Atlantic salar is greatly reduced, and the treatment effect reaches 93% or above; the infection of the healthy salar is prevented, and the probability of injury to the Atlantic salar by the saprolegniasis fungi is reduced.

Description

technical field [0001] The invention relates to the technical field of Atlantic salmon cultivation, in particular to a fern extract for preventing and treating saprolegniasis of Atlantic salmon and its application. Background technique [0002] Atlantic salmon (Salmon salar L.), belonging to the order Salmoniformes, the family Salmonoidea, and the genus Salmon, is commonly known as "salmon", and is widely distributed in the temperate and frigid waters of the North Atlantic Ocean. Atlantic salmon has the characteristics of large body, good meat quality, high content of unsaturated fatty acids, fast growth in a lower water temperature environment, and high-density farming, making it the cold-water fish with the highest yield in artificial farming. kind. [0003] However, in the process of raising seedlings, when a series of operations such as weighing, pouring tanks, dividing fish, and vaccinating are carried out, some fry may be damaged, and Saprolegnia will take the opportu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/12A61P31/10
CPCA61K36/12A61K2236/33A61K2236/333A61K2236/39A61K2236/51A61K2300/00
Inventor 冯碧凤贺兵雷波
Owner 铜仁市万山区水产站
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