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Microfluidic device, system and method for introducing substances into cells

A microfluidic device and cell technology, applied in the biological field, can solve the problems of unstable cell shear force, inability to accurately control the distance between cells and bubbles, etc.

Active Publication Date: 2020-06-12
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of this, the purpose of the embodiments of the present invention is to provide a microfluidic device, a system and a method for introducing substances into cells, so as to solve the problem that the equipment used for sonoporation in the prior art cannot precisely control the gap between cells and air bubbles. distance, thus destabilizing the shear stress on the cells

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  • Microfluidic device, system and method for introducing substances into cells
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Embodiment approach 1

[0064] The invention relates to a microfluidic device, comprising:

[0065] a cavity having microstructures disposed on its inner wall, the microstructures being configured such that when the solution is injected into the cavity, the solution forms bubbles at the microstructures; and,

[0066] The body wave generating device is used to generate body waves to resonate the above-mentioned bubbles to generate a flow field.

[0067] In one embodiment, the microfluidic device further includes a surface acoustic wave generating device for generating surface acoustic waves and controlling the position of particles in the solution.

[0068] An embodiment of the present invention provides a microfluidic device, such as figure 1 As shown, the device includes a cavity 110, a bulk wave generating device 120 and a surface acoustic wave generating device 130;

[0069] The interior of the cavity 110 is provided with microstructures, and when the solution is injected into the cavity 110, th...

Embodiment approach 2

[0110] Embodiments of the present invention provide a system for introducing substances into cells, such as Figure 5 As shown, the system includes an injection device 510 and the microfluidic device 520 provided in Embodiment 1 above;

[0111] The injection device 510 is used to inject a solution containing cells into the cavity of the microfluidic device 520, and the injected solution generates bubbles at the microstructure of the cavity;

[0112] The above-mentioned microfluidic device 520 is used to generate body waves, make the above-mentioned bubbles resonate, cause the vibration of the solution in the cavity, generate a flow field in the cavity, and each position in the flow field corresponds to a different shear stress, and control the cells in the cavity. The position of the flow field relative to the microstructure makes the cells flow through the preset position when flowing in the flow field, and the cells generate reversible perforation under the action of the she...

Embodiment approach 3

[0127] An embodiment of the present invention provides a method for introducing substances into cells. This method applies the system for introducing substances into cells in the above-mentioned embodiment 2. In this method, the body wave generation device in the microfluidic device can precisely control the flow of cells. The position in the field relative to the microstructure makes each cell pass a preset position when flowing in the flow field, thereby precisely controlling the shear stress on the cells.

[0128] Among them, the substances introduced into the cells in the embodiment of the present invention can be gene fragments, drug molecules, etc., and the user can put the substances to be introduced into the cells into the solution according to actual needs, and then use the method provided in the embodiments of the present invention to introduce the substances into the cells.

[0129] Using the method provided by the embodiments of the present invention to introduce su...

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Abstract

The invention provides a microfluidic device, a system and a method for introducing substances into cells, wherein the microfluidic device includes a cavity, a body wave generating device and a surface acoustic wave generating device; the inner wall of the cavity is provided with The microstructure is configured such that when the solution is injected into the cavity, the solution forms bubbles at the microstructure; the body wave generating device is used to generate body waves to resonate the bubbles to generate a flow field; the A surface acoustic wave generating device for generating surface acoustic waves and controlling the position of particles in the solution. In the present invention, the position of the particles in the solution can be precisely controlled by means of the surface acoustic wave generating device, thereby realizing precise control of the shear stress on the particles in the solution.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microfluidic device, a system method and a method for introducing substances into cells. Background technique [0002] With the introduction of gene therapy theory and continuous in-depth research, human beings have entered a new stage of development in the study of thoroughly overcoming malignant tumors, hereditary diseases, and infectious diseases. The method is to introduce normal genes or genes with therapeutic effects into human target cells to correct gene defects or functional disorders, so as to achieve the purpose of treating diseases. How to efficiently and safely deliver exogenous substances from extracellular to intracellular is the key to gene therapy. [0003] At present, the methods for introducing substances into cells include viral gene introduction, injection, gene gun method, electroporation and sonoporation. The cells are shaken to make the bubbles evenly scat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/87B01L3/00
CPCC12N15/87B01L3/5027B01L2200/0647B01L2400/0436B01L3/502761B01L2400/0496B01L3/50273B01L3/502715B01L2400/0439B01L3/508B01L2300/123B01L2300/168C12N15/895
Inventor 郑海荣周伟孟龙牛丽丽林争荣王凯悦黄小伟
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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