Test strip for immunochromatography-based rationed joint detection of C-reactive protein and serum amyloid A and manufacture method of test strip

Abstract

The invention discloses a test strip for immunochromatography-based rationed joint detection of C-reactive protein and serum amyloid A and a manufacture method of the test strip. The test strip comprises a clamp shell, a bottom plate, a sample pad, a fluorescence indicator combination pad, a nitrocellulose membrane and a water-absorbing pad, and is formed by sequentially adhering the sample pad, the fluorescence indicator combination pad, the nitrocellulose membrane and the water-absorbing pad to the bottom plate in a lap joint mode. The fluorescence indicator combination pad comprises streptavidin-labeled fluorescent protein, biotin-labeled C-reactive protein monoclonal antibodies, and biotin-labeled serum amyloid A monoclonal antibodies. Detection lines and quality control lines are arranged on the nitrocellulose membrane. The test strip and the manufacture method thereof have the advantages that the rapid rationed joint detection of the C-reactive protein/the serum amyloid A is realized through combination between a fluorescent protein indicator and a 'streptavidin-biotin amplification system'; detection time is shortened greatly, detection sensitivity is improved, and convenience and reliability are improved greatly through joint detection.

Description

technical field [0001] The invention belongs to the field of immune detection, and in particular relates to a high-sensitivity C-reactive protein and serum amyloid A immunochromatographic quantitative joint detection test strip and a preparation method thereof. Background technique [0002] C-reactive protein (CRP) is a very sensitive marker of inflammation and tissue damage. In various acute or chronic infections, tissue damage, myocardial infarction, etc., CRP tends to increase exponentially within a few hours after the disease occurs. It can even be as high as 2000 times, and it grows faster and significantly than other phase proteins, and its increase is positively correlated with the degree of infection. It can be used as an early diagnosis indicator of infection and can also be used for clinical monitoring of disease course. CRP usually increases after bacterial infection, but does not increase or slightly increases after viral infection. Therefore, CRP is clinically u...

AI Technical Summary

Problems solved by technology

There is no report on the development of rapid diagnostic reagents

Images